Spring 2011:Optical Trapping Lab

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20.309: Biological Instrumentation and Measurement

ImageBar 774.jpg

Team FTS

Huayu & Cory

XY position detector calibration

check to see the direction of scan (X)
check to see the direction of scan (Y)
Xcal - volt Vs. position center slope = -3.457
Ycal - volt Vs. position center slope = m = -7.816

Roll Off

Power of laser 10mW, Calculated stiffness =9.28E-6 N/m = .00926 pN/nm

Stoke

StokeTeamFTS.png

Equipartition

for 10 mW : 2.09*10^-9 N/m

Team Humble

XY position detector calibration

Xcallibration.jpg

Trap stiffness by equipartition theorem

Method1 stiffness.jpg

These values are off by about 8 orders of magnitude. This graph is up temporarily until I figure out what is going wrong with my calculations.

Trap stiffness by PSD rolloff

Method2 stiffness.jpg

These values are about 2 orders of magnitude larger than those found in the OTKB document.

Trap stiffness by Stokes' theorem

Method3 stiffness.jpg

These values are about the correct order of magnitude, but the slope of the graph should be positive, not negative! Again, this graph is up temporarily until I can figure out what data processing mistakes are going on.

Team Watson (Brian)

XY position detector calibration

Sens x.jpg Sens y.jpg

Although the Y axis plot looks OK, I'm not sure of what happened to the X axis one

Trap stiffness by equipartition theorem

Eq x.jpg Eq y.jpg

The X-axis plot follows the expected trend of higher stiffness with higher power, but the Y axis has a very off-line measurement, although the orders of magnitude seem right.

Trap stiffness by PSD rolloff

PSD x.jpg PSD y.jpg

Both of these plots are right around the expected value, although they are around twice the values found for the Equipartition method (maybe there are some conversion errors).

Trap stiffness by Stokes' theorem

Stokes x.jpg Stokes y.jpg

Finally, this method seems to follow the expected trend, but its values are around an order of magnitude off from the previous methods. Again, this may be due to unit conversion issues (currently revising).

Team Watson (Emmanuel)

XY position detector calibration

QPD eq.jpg

  • QPD_XY[V/um] = 3.38e-4 * Power[mW] + 1.6e-3
  • QPD_YX[V/um] = 4.60e-4 * Power[mW] + 8.5e-3

Trap stiffness by equipartition theorem

Equipart eq.jpg

  • Equipart_stiff for X axis: kg/s^2 = 4.57e-16 * Power[mW] + 5.14e-16
  • Equipart_stiff for Y axis: kg/s^2 = 3.37e-16 * Power[mW] + 3.85e-16

Trap stiffness by PSD rolloff

PSD.jpg

  • PSD roll-off stiff for X axis: kg/s^2 = 1.8e-15 * Power[mW] - 51.6e-15
  • PSD roll-off stiff for Y axis: kg/s^2 = 1.9e-15 * Power[mW] - 60.6e-15

Trap stiffness by Stokes' theorem