Spring 2011:Optical Trapping Lab

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20.309: Biological Instrumentation and Measurement

ImageBar 774.jpg

Team Humble

XY position detector calibration

Xcallibration.jpg

Trap stiffness by equipartition theorem

Method1 stiffness.jpg

These values are off by about 8 orders of magnitude. This graph is up temporarily until I figure out what is going wrong with my calculations.

Trap stiffness by PSD rolloff

Method2 stiffness.jpg

These values are about 2 orders of magnitude larger than those found in the OTKB document.

Trap stiffness by Stokes' theorem

Method3 stiffness.jpg

These values are about the correct order of magnitude, but the slope of the graph should be positive, not negative! Again, this graph is up temporarily until I can figure out what data processing mistakes are going on.