Difference between revisions of "Spring 11:Directed Evolution"
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| + | |[[File:Spartan-laser.jpg|350px]] | ||
| + | |style="padding-left: 30px; font-size:45px"|'''''Directed Evolution''''' | ||
| + | |} | ||
| − | ==Project | + | ==Project Information== |
| + | ===Objective=== | ||
| + | The objective of this project is to constructed an automated directed evolution apparatus for long term evolution and optimization studies. Conducted as final project for [[20.345:Course_Information|20.345]] Spring 2011. | ||
| − | == | + | ===Goals=== |
| + | * '''Primary''' - demonstrate targeted cellular ablation | ||
| + | * '''Secondary''' - implement ablation scanning optics / mechanics | ||
| + | * '''Tertiary''' - build software for long-term time studies | ||
| + | ===People=== | ||
| + | '''Students''' | ||
| + | * Cory Li (''coryli'') | ||
| + | * Huayu Ding (''huayud'') | ||
| + | '''Mentor''' | ||
| + | * Professor Yanik | ||
| − | == | + | ===Notes=== |
| + | * [[Spring 11:Directed Evolution/Perfusion Chambers|Perfusion Chambers]] | ||
| + | * [[Spring 11:Directed Evolution/KAF95|KAF95 Strain]] | ||
| + | ==Status Reports== | ||
| + | * [[Spring 11:Directed Evolution/Week of 3-14-2011 Status|Week of 3-14-2011]] | ||
| + | * [[Spring 11:Directed Evolution/Week of 3-21-2011 Status|Week of 3-21-2011]] | ||
| + | * [[Spring 11:Directed Evolution/Week of 3-28-2011 Status|Week of 3-28-2011]] | ||
| + | * [[Spring 11:Directed Evolution/Week of 4-04-2011 Status|Week of 4-04-2011]] | ||
| + | * [[Spring 11:Directed Evolution/Week of 4-11-2011 Status|Week of 4-11-2011]] | ||
| + | * [[Spring 11:Directed Evolution/Week of 4-18-2011 Status|Week of 4-18-2011]] | ||
| + | * [[Spring 11:Directed Evolution/Week of 4-25-2011 Status|Week of 4-25-2011]] | ||
| + | * [[Spring 11:Directed Evolution/Week of 5-02-2011 Status|Week of 5-02-2011]] | ||
| + | * [[Spring 11:Directed Evolution/Week of 5-09-2011 Status|Week of 5-09-2011]] | ||
| + | ==Useful References== | ||
| + | ====Laser Ablation==== | ||
| + | * [http://link.aip.org/link/?PSI/4260/109/1&Agg=doi Shen, Feimo. 2001. “Laser ablation cell sorting in scanning cytometry.” Proceedings of SPIE 4260: 109-119.] | ||
| + | * [http://www.springerlink.com/index/10.1007/s00340-005-2036-6. Vogel, a. et al. 2005. “Mechanisms of femtosecond laser nanosurgery of cells and tissues.” Applied Physics B 81(8): 1015-1047.] | ||
| + | |||
| + | ====Evolutionary Theory==== | ||
| + | * [http://www.amazon.com/Evolutionary-Dynamics-Exploring-Equations-Life/dp/0674023382 Nowak. 2006. Evolutionary Dynamics] | ||
==Links== | ==Links== | ||
| + | * [https://bitbucket.org/Cixelyn BitBucket Code Repo] | ||
Latest revision as of 00:54, 28 April 2011
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Directed Evolution |
Project Information
Objective
The objective of this project is to constructed an automated directed evolution apparatus for long term evolution and optimization studies. Conducted as final project for 20.345 Spring 2011.
Goals
- Primary - demonstrate targeted cellular ablation
- Secondary - implement ablation scanning optics / mechanics
- Tertiary - build software for long-term time studies
People
Students
- Cory Li (coryli)
- Huayu Ding (huayud)
Mentor
- Professor Yanik
Notes
Status Reports
- Week of 3-14-2011
- Week of 3-21-2011
- Week of 3-28-2011
- Week of 4-04-2011
- Week of 4-11-2011
- Week of 4-18-2011
- Week of 4-25-2011
- Week of 5-02-2011
- Week of 5-09-2011
Useful References
Laser Ablation
- Shen, Feimo. 2001. “Laser ablation cell sorting in scanning cytometry.” Proceedings of SPIE 4260: 109-119.
- Vogel, a. et al. 2005. “Mechanisms of femtosecond laser nanosurgery of cells and tissues.” Applied Physics B 81(8): 1015-1047.
