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- 20.109(F13): DNA engineering methods
- 20.109(F13): DNA engineering summary
- 20.109(F13): Fall 2013 schedule
- 20.109(F13): Mod 1 Day 1 DNA engineering using PCR
- 20.109(F13): Mod 1 Day 2 Clean and cut DNA
- 20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis
- 20.109(F13): Mod 1 Day 4 DNA ligations and bacterial transformations
- 20.109(F13): Mod 1 Day 5 Examine candidate clones & tissue culture
- 20.109(F13): Mod 1 Day 6 Lipofection and paper discussion
- 20.109(F13): Mod 1 Day 7 FACS analysis
- 20.109(F13): Mod 2 Day 1 System Design
- 20.109(F13): Mod 2 Day 2 Mutation Analysis
- 20.109(F13): Mod 2 Day 2 Tools for Systems Biology
- 20.109(F13): Mod 2 Day 3 Prepare for LTS
- 20.109(F13): Mod 2 Day 4 Phosphotyrosine Western Blot Analysis
- 20.109(F13): Mod 2 Day 6 Analysis & Planning 2
- 20.109(F13): Mod 2 Day 6 Transfection of SH2 Domains
- 20.109(F13): Mod 2 Day 7 HTS and Analysis
- 20.109(F13): Mod 3 Day 1 Growth of phage materials
- 20.109(F13): Mod 3 Day 2 Phage nanowires
- 20.109(F13): Mod 3 Day 3 TEM
- 20.109(F13): Mod 3 Day 4 Solar cell assembly
- 20.109(F13): Mod 3 Day 5 Solar cell testing
- 20.109(F13): Mod 3 Day 6 Wrap-up and data summary
- 20.109(F13): Mod 3 Day 7 Oral presentations
- 20.109(F13): System engineering report
- 20.109(F13): TA notes for module 1
- 20.109(F13): TA notes for module 2
- 20.109(F13): Tools for Systems Biology (Day2)
- 20.109(F14)
- 20.109(F14):20.109 Class Blog summary page
- 20.109(F14):General lab policy
- 20.109(F14):Guidelines for editing OpenWetWare
- 20.109(F14):Guidelines for maintaining your lab notebook
- 20.109(F14):Guidelines for oral presentations
- 20.109(F14):Guidelines for working in the tissue culture facility
- 20.109(F14):Guidelines for writing up your research
- 20.109(F14):Journal club II (Day8)
- 20.109(F14):Lab basics
- 20.109(F14):Lab tour
- 20.109(F14):Module 1
- 20.109(F14):Module 2
- 20.109(F14):Module 3
- 20.109(F14):Module 3 oral presentations
- 20.109(F14):OWW basics
- 20.109(F14):OWW in 20.109
- 20.109(F14):OpenWetWare
- 20.109(F14):People
- 20.109(F14):Statement on collaboration and integrity
- 20.109(F14): Assignments
- 20.109(F14): Biomaterial engineering report
- 20.109(F14): DNA engineering methods
- 20.109(F14): DNA engineering summary
- 20.109(F14): Fall 2012 schedule
- 20.109(F14): Fall 2014 schedule
- 20.109(F14): Journal Club I
- 20.109(F14): Journal Club Presentations
- 20.109(F14): Mod 1 Day 1 DNA engineering using PCR
- 20.109(F14): Mod 1 Day 2 Clean and cut DNA
- 20.109(F14): Mod 1 Day 3 Agarose gel electrophoresis
- 20.109(F14): Mod 1 Day 4 DNA Ligation & Transformation
- 20.109(F14): Mod 1 Day 4 DNA ligations, bacterial transformations and CometChip
- 20.109(F14): Mod 1 Day 4 DNA ligations and bacterial transformations
- 20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture
- 20.109(F14): Mod 1 Day 6 Lipofection
- 20.109(F14): Mod 1 Day 6 Lipofection and CometChip Analysis
- 20.109(F14): Mod 1 Day 6 Lipofection and paper discussion
- 20.109(F14): Mod 1 Day 7 FACS analysis
- 20.109(F14): Mod 2 Day 1 Testing an engineered biological system
- 20.109(F14): Mod 2 Day 2 Measuring system performance
- 20.109(F14): Mod 2 Day 3 Tools for system engineering
- 20.109(F14): Mod 2 Day 5 Assessing re-tuned system
- 20.109(F14): Mod 2 Day 6 Readouts of DNA, Protein
- 20.109(F14): Mod 2 Day 7 Readout of Protein
- 20.109(F14): Mod 3 Day 1 Growth of phage materials
- 20.109(F14): Mod 3 Day 2 Phage nanowires
- 20.109(F14): Mod 3 Day 3 TEM
- 20.109(F14): Mod 3 Day 4 Solar cell assembly
- 20.109(F14): Mod 3 Day 5 Solar cell testing
- 20.109(F14): Mod 3 Day 6 Wrap-up and data summary
- 20.109(F14): Mod 3 Day 7 Oral presentations
- 20.109(F14): Practice Virtual Lab
- 20.109(F14): Slides for Journal Club Presentation
- 20.109(F14): System engineering report
- 20.109(F14): System engineering research article
- 20.109(F14): TA's notes for module 3
- 20.109(F14): TA notes for module 1
- 20.109(F14): TA notes for module 2
- 20.109(F14): TA notes for orientation
- 20.109(F15)
- 20.109(F15):20.109 Class Blog summary page
- 20.109(F15):Agarose gel electrophoresis (Day3)
- 20.109(F15):Assess protein function (Day7)
- 20.109(F15):Bacterial amplification of DNA (Day3)
- 20.109(F15):Battery assembly (Day3)
- 20.109(F15):Battery assembly and testing (Day5)
- 20.109(F15):Battery testing (Day4)
- 20.109(F15):Cathode construction (Day3)
- 20.109(F15):Characterize protein expression (Day6)
- 20.109(F15):Clean and cut DNA (Day2)
- 20.109(F15):DNA engineering using PCR (Day1)
- 20.109(F15):DNA ligation & transformation (Day4)
- 20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)
- 20.109(F15):Examine candidate clones & tissue culture (Day5)
- 20.109(F15):FACS analysis (Day7)
- 20.109(F15):General lab policy
- 20.109(F15):Growth of phage materials (Day1)
- 20.109(F15):Guidelines for maintaining your lab notebook
- 20.109(F15):Guidelines for oral presentations
- 20.109(F15):Guidelines for working in the tissue culture facility
- 20.109(F15):Guidelines for writing up your research
- 20.109(F15):Homework
- 20.109(F15):Induce protein and evaluate DNA (Day3)
- 20.109(F15):Journal Club II (Day8)
- 20.109(F15):Journal Club I (Day5)
- 20.109(F15):Lab basics
- 20.109(F15):Lab tour
- 20.109(F15):Lipofection (Day6)
- 20.109(F15):Module 1
- 20.109(F15):Module 2
- 20.109(F15):Module 3
- 20.109(F15):People
- 20.109(F15):Phage nanowires (Day2)
- 20.109(F15):Prepare expression system (Day2)
- 20.109(F15):Purify active materials (Day2)
- 20.109(F15):Purify protein (Day4)
- 20.109(F15):Research proposal presentation
- 20.109(F15):Research proposal presentations (Day6)
- 20.109(F15):Site-directed mutagenesis (Day2)
- 20.109(F15):Statement on collaboration and integrity
- 20.109(F15):Student registration and questionnaire
- 20.109(F15):TEM (Day4)
- 20.109(F15):TEM (Day5)
- 20.109(F15):Wiki basics
- 20.109(F15): Assignments
- 20.109(F15): Biomaterials engineering report
- 20.109(F15): DNA engineering methods
- 20.109(F15): DNA engineering presentation
- 20.109(F15): DNA engineering summary
- 20.109(F15): Fall 2015 schedule
- 20.109(F15): Protein engineering report
- 20.109(F15): TA's notes for module 3
- 20.109(F15): TA notes for module 1
- 20.109(F15): TA notes for module 2
- 20.109(F15): TA notes for orientation
- 20.109(F16)::Measure fermentation products (Day8)
- 20.109(F16):Agarose gel electrophoresis (Day3)
- 20.109(F16):Asses DNA repair variability with comet chip (Day4)
- 20.109(F16):Battery assembly and testing (Day5)
- 20.109(F16):Cathode construction (Day3)
- 20.109(F16):Clean and cut DNA (Day2)
- 20.109(F16):Communication
- 20.109(F16):Complete immuno-fluorescence assay (Day6)
- 20.109(F16):Complete in silico cloning (Day1)
- 20.109(F16):Confirm gRNA sequence (Day5)
- 20.109(F16):DNA engineering using PCR (Day1)
- 20.109(F16):DNA ligation & transformation (Day4)
- 20.109(F16):Data analysis (Day7)
- 20.109(F16):Design gRNA for CRISPRi (Day2)
- 20.109(F16):Examine candidate clones & tissue culture (Day5)
- 20.109(F16):FACS analysis (Day7)
- 20.109(F16):Generate gRNA plasmid (Day3)
- 20.109(F16):Growth of phage materials (Day1)
- 20.109(F16):Homework
- 20.109(F16):Induce CRISPRi system (Day7)
- 20.109(F16):Journal club II (Day 6)
- 20.109(F16):Journal club I (Day4)
- 20.109(F16):Lab tour
- 20.109(F16):Lipofection (Day6)
- 20.109(F16):Measure DNA damage with comet chip (Day3)
- 20.109(F16):Module 1
- 20.109(F16):Module 2
- 20.109(F16):Module 3
- 20.109(F16):Prepare comet chips (Day1)
- 20.109(F16):Purify active materials (Day2)
- 20.109(F16):Research proposal presentation
- 20.109(F16):Seed cells for immuno-flourescence assay (Day5)
- 20.109(F16):Statement on collaboration and integrity
- 20.109(F16):TEM (Day4)
- 20.109(F16):Test comet chip assay variables (Day3)
- 20.109(F16):Test comet chip loading variables (Day2)
- 20.109(F16): Announcements
- 20.109(F16): Assignments
- 20.109(F16): Biomaterials engineering mini-report
- 20.109(F16): DNA engineering presentation
- 20.109(F16): DNA engineering summary
- 20.109(F16): Fall 2016 schedule
- 20.109(F16): M1 Data Summary
- 20.109(F16): M1 Mini-presentation
- 20.109(F16): Research article
- 20.109(F16): TA notes for M1
- 20.109(F16): TA notes for M12
- 20.109(F16): TA notes for M2
- 20.109(F16): TA notes for M3
- 20.109(F16): TA notes for orientation
- 20.109(F17)::Measure fermentation products (Day8)
- 20.109(F17):Assemble and test batteries (Day5)
- 20.109(F17):Cathode construction (Day3)
- 20.109(F17):Communication
- 20.109(F17):Complete biochemical experiment (Day5)
- 20.109(F17):Complete in silico cloning (Day1)
- 20.109(F17):Confirm gRNA sequence (Day5)
- 20.109(F17):Design gRNA for CRISPRi (Day2)
- 20.109(F17):Develop experiment to optimize loading variables (Day2)
- 20.109(F17):Evaluate cell loading results (Day3)
- 20.109(F17):Examine sub-nuclear foci abundance to measure DNA damage (Day6)
- 20.109(F17):Generate gRNA plasmid (Day3)
- 20.109(F17):Growth of phage materials (Day1)
- 20.109(F17):Homework
- 20.109(F17):Induce CRISPRi system (Day7)
- 20.109(F17):Journal club II (Day 6)
- 20.109(F17):Journal club I (Day4)
- 20.109(F17):Lab tour
- 20.109(F17):Module 1
- 20.109(F17):Module 2
- 20.109(F17):Module 3
- 20.109(F17):Prepare comet chips (Day1)
- 20.109(F17):Purify active materials (Day2)
- 20.109(F17):Research proposal presentation
- 20.109(F17):Test role of biochemical factors in genomic stability (Day4)
- 20.109(F17):Visualize active material using TEM (Day4)
- 20.109(F17):Visualize and analyze data for sub-nuclear foci assay (Day7)
- 20.109(F17): Announcements
- 20.109(F17): Assignments
- 20.109(F17): Biomaterials engineering mini-report
- 20.109(F17): Fall 2017 Schedule
- 20.109(F17): M1 Data Summary
- 20.109(F17): M1 Mini-presentation
- 20.109(F17): Notes for M1
- 20.109(F17): Notes for orientation
- 20.109(F17): Research article
- 20.109(F18):Analyze genomic instability experiment data and treat cells for sub-nuclear foci assay (Day5)
- 20.109(F18):Assemble and test batteries (Day5)
- 20.109(F18):Class data
- 20.109(F18):Communication
- 20.109(F18):Complete genomic instability experiment and load cells for sub-nuclear foci assay (Day4)
- 20.109(F18):Complete in silico cloning (Day1)
- 20.109(F18):Complete sub-nuclear foci assay (Day6)
- 20.109(F18):Confirm gRNA sequence (Day5)
- 20.109(F18):Construct cathode with phage-based active material (Day3)
- 20.109(F18):Design cell loading optimization experiment and discuss genomic instability experiment (Day2)
- 20.109(F18):Design gRNA for CRISPRi (Day2)
- 20.109(F18):Evaluate cell loading experiment and treat cells for genomic instability experiment (Day3)
- 20.109(F18):Generate gRNA plasmid (Day3)
- 20.109(F18):Growth of phage-based active (cathode) material (Day1)
- 20.109(F18):Homework
- 20.109(F18):Image sub-nuclear foci assay
- 20.109(F18):Image sub-nuclear foci assay (Day6)
- 20.109(F18):Induce CRISPRi system (Day7)
- 20.109(F18):Journal club presentation (Day4 and 6)
