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  • Final Project: Spatial Light Modulator Galore
    #REDIRECT [[A.Williams: Objectives and Literature Review]]
    58 B (7 words) - 14:39, 11 March 2012
  • Spring 2012:LFM Documentation
    ...llows the consumer to refocus the image before capturing a light field, to review all past photos still on the camera, and to refocus after capturing the ima
    18 KB (2,796 words) - 05:15, 18 May 2012
  • Nonlinear regression
    ==Review of linear regression==
    5 KB (774 words) - 18:07, 13 January 2014
  • Electronics Mini-Lab
    This bootcamp requires an understanding of basic circuits. If you need to review circuit concepts, start with the [[Electronics Primer]] page. If you have a
    35 KB (5,891 words) - 18:46, 7 April 2017
  • Geometrical Optics
    [[Category:Needs review]] ==Concept review==
    793 B (95 words) - 21:35, 4 June 2015
  • Error analysis
    ...l. Fortunately, there always seems to be somebody with a bigger office who needs a couch. ==Review of probability and statists concepts==
    24 KB (3,757 words) - 14:57, 3 September 2018
  • Optics Bootcamp
    For a good review of geometrical optics, ray tracing, and some worked-out ray tracing example
    28 KB (4,857 words) - 20:09, 31 July 2017
  • Estimating second order system parameters from noise power spectra using nonlinear regression
    ==Regression concepts review==
    30 KB (4,815 words) - 21:47, 27 December 2015
  • 20.109(S08):Transcript-level analysis (Day5)
    ... topic). Do your best to incorporate suggestions from the peer and faculty review.
    14 KB (2,255 words) - 16:04, 15 June 2015
  • 20.109(S08):Initiate cell culture (Day2)
    ...l-biomaterial engineering. During lab next time, you and your partner will review the topics and narrow your choices, identifying one or perhaps two topics f
    12 KB (2,006 words) - 16:04, 15 June 2015
  • 20.109(S08):Preparing cells for analysis (Day4)
    ...ab partner. Begin by reading the [[Media:PeerReviewTips.pdf| tips for peer review]] from Neal Lerner. Then, as you read your lab partner's writing, be a thou ...uggestions for improvement whenever possible. Print out two copies of your review, one to hand in and one for your lab partner.
    16 KB (2,751 words) - 16:04, 15 June 2015
  • 20.109(S08):Wrap-up analysis (Day7)
    <i>As you can see from this image, taken from a review on hydrocarbon metabolism in marine bacteria, the alcanivorax species is th
    7 KB (1,208 words) - 16:04, 15 June 2015
  • 20.109(S08):DNA engineering/DNA engineering by PCR (Day 1)
    ...basic information on homologous recombination, please obtain the excellent review by Thomas Helleday from the References section of the [http://openwetware.o
    27 KB (3,752 words) - 14:24, 5 June 2015
  • 20.109(S08):DNA engineering/Clean and cut DNA (Day 2)
    ...ure of biological engineering, integrity in scientific publishing and peer review, and trends and regulation in the biotechnology industry, among others. Be
    10 KB (1,692 words) - 14:24, 5 June 2015
  • 20.109(S08):DNA engineering/Restriction map and tissue culture (Day 6)
    ... minutes) and the gel will be photographed. While you are waiting, you can review and discuss the assigned article.
    11 KB (1,738 words) - 14:24, 5 June 2015
  • 20.109(S08):Site-directed mutagenesis (Day2)
    ...ool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum this review by David Clapham in Cell], or [http://www.ncbi.nlm.nih.gov/pubmed/11830654?
    12 KB (1,954 words) - 14:24, 5 June 2015
  • 20.109(S08):Student presentations (M2D8)
    ====Review Articles (for reference only)==== ====Review Articles (for reference only)====
    9 KB (1,472 words) - 14:24, 5 June 2015
  • 20.109(S08):Lab tour
    # Review todays lab to prepare for the lab practical that you and your partner wi
    22 KB (3,631 words) - 14:24, 5 June 2015
  • 20.109(S08):Guidelines for oral presentations
    *Review each of your main messages
    5 KB (761 words) - 14:25, 5 June 2015
  • 20.109(S08):Guidelines for writing a lab report
    ...e way a journal’s editor decides whether to send your paper out for peer review or reject it as uninteresting and not generally relevant. Consequently, a w
    12 KB (1,912 words) - 14:25, 5 June 2015
  • 20.109(S08):Data analysis (Day7)
    ...nable titration curves by following the example of Nagai, the introduction/review of binding constants below may help contextualize your analysis.
    14 KB (2,336 words) - 14:25, 5 June 2015
  • 20.109(S07): Yeast transformation
    ...distinguish these possible explanations but it can tell you that something needs to be re-worked. ...s important work on the structure of the SAGA complex. You might find this review helpful but you are not required to read it. <br>
    19 KB (3,251 words) - 15:32, 15 June 2015
  • 20.109(S07):Lab tour
    # Review todays lab to prepare for the lab practical that you and your partner wi
    22 KB (3,724 words) - 15:32, 15 June 2015
  • 20.109(S07):Guidelines for oral presentations
    *Review each of your main messages
    4 KB (732 words) - 15:32, 15 June 2015
  • 20.109(S07):Guidelines for writing a lab report
    ...e way a journal’s editor decides whether to send your paper out for peer review or reject it as uninteresting and not generally relevant. Consequently, a w
    12 KB (1,849 words) - 15:32, 15 June 2015
  • Bryan Hernandez/20.109/Lab notebook/Module 2/Day 2
    ...ve&dopt=AbstractPlus&list_uids=12542690&query_hl=6&itool=pubmed_docsum the review by Vetter and LeClerc]) in FEBS 2003. <br>
    19 KB (3,069 words) - 15:48, 15 June 2015
  • 20.109(S07): Colony PCR
    ...rlVersion=0&_userid=501045&md5=b1c5e4e2f9e0094a16d7b6ac47413f7f associated review article] that was written to celebrate and highlight this important work on
    9 KB (1,466 words) - 15:32, 15 June 2015
  • 20.109(S07): Growth of phage materials
    ... bio-material engineering. During lab next time, you and your partner will review the topics and narrow your choices, identifying one or perhaps two topics f
    4 KB (600 words) - 15:32, 15 June 2015
  • 20.109(S07):Bio-material engineering/Screening library
    ... bio-material engineering. During lab next time, you and your partner will review the topics and narrow your choices, identifying one or perhaps two topics f
    9 KB (1,473 words) - 15:31, 15 June 2015
  • Bryan Hernandez/20.109/Lab notebook/Module 1/Day 2
    #Begin by looking at the primer record you wrote last time and review your planning of the myc-tag that you designed. Do the single-stranded over
    17 KB (2,960 words) - 15:31, 15 June 2015
  • 20.109(S07): Agarose gel electrophoresis
    #Begin by looking at the primer record you wrote last time and review your planning of the myc-tag that you designed. Do the single-stranded over
    15 KB (2,609 words) - 15:31, 15 June 2015
  • 20.109(S07): Start-up signal measurement
    #Review your understanding of protein structure visualization by examining another
    21 KB (3,477 words) - 15:31, 15 June 2015
  • 20.109(S07): Measuring calcium in vitro
    ...ve&dopt=AbstractPlus&list_uids=12542690&query_hl=6&itool=pubmed_docsum the review by Vetter and LeClerc]) in FEBS 2003. <br>
    20 KB (3,237 words) - 15:45, 15 June 2015
  • 20.109(S07): Lipofection
    # Review the descriptions of the lab's microscopes that we covered during [http://op
    9 KB (1,436 words) - 15:31, 15 June 2015
  • 20.109(S07): Measuring calcium in vivo
    ...]]. You will be presenting one of these articles to the class, so begin to review both the [[20.109(S07):Guidelines for oral presentations| guidelines for or
    7 KB (1,103 words) - 15:31, 15 June 2015
  • 20.109(S07): Bio-material engineering/Optimizing panning
    #To better understand how the library of plasmids was synthesized, please review the chemistry involved in joining nucleotides during DNA replication. Draw
    10 KB (1,637 words) - 15:31, 15 June 2015
  • 20.109(F07):Guidelines for writing a lab report
    ...e way a journal’s editor decides whether to send your paper out for peer review or reject it as uninteresting and not generally relevant. Consequently, a w
    12 KB (1,850 words) - 15:54, 15 June 2015
  • 20.109(F07):Lab tour
    # Review todays lab to prepare for the lab practical that you and your partner wi
    22 KB (3,670 words) - 15:54, 15 June 2015
  • 20.109(F07): M13.1
    ...: [http://parts.mit.edu/registry/index.php/Help:Ribosome_Binding_Site this review site] which includes some useful characterization information. <font color ... the point of the sentence hard to get"</i>). Print out two copies of your review, one to hand in and one for your lab partner. Keep your fingers crossed tha
    19 KB (3,059 words) - 16:00, 15 June 2015
  • 20.109(F07):Guidelines for oral presentations
    *Review each of your main messages
    4 KB (733 words) - 15:54, 15 June 2015
  • 20.109(F07): Genome engineering assessment
    ...ext time| a homework assignment], exchanged with your lab partner for peer review, then submitted to the teaching faculty as part of your portfolio. <b>This
    15 KB (2,532 words) - 15:54, 15 June 2015
  • 20.109(F07): Module 2 oral presentations
    ...s=4&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum review's abstract and pubmed link]
    11 KB (1,596 words) - 15:54, 15 June 2015
  • 20.109(F07): Growth of phage materials
    ...earch tool for finding peptides that interact with materials to meet human needs. Phage that can bind to cobalt oxide, gold, iridium and indium tin oxide ar ... bio-material engineering. During lab next time, you and your partner will review the topics and narrow your choices, identifying one or perhaps two topics f
    10 KB (1,707 words) - 15:54, 15 June 2015
  • 20.109(F08):Lab tour
    # Review todays lab to prepare for the lab practical that you and your partner wi
    26 KB (4,345 words) - 19:05, 28 July 2015
  • 20.109(F08): Mod 3 Day 7 Oral presentations
    <i>As you can see from this image, taken from a review on hydrocarbon metabolism in marine bacteria, the alcanivorax species is th <i>As you can see from this image, taken from a review on hydrocarbon metabolism in marine bacteria, the alcanivorax species is th
    12 KB (1,972 words) - 19:05, 28 July 2015
  • 20.109(F08): Yeast transformation
    ...distinguish these possible explanations but it can tell you that something needs to be re-worked. ...s important work on the structure of the SAGA complex. You might find this review helpful but you are not required to read it. <br>
    19 KB (3,254 words) - 19:05, 28 July 2015
  • 20.109(F08):DNA engineering/Clean and cut DNA (Day 2)
    ...ure of biological engineering, integrity in scientific publishing and peer review, and trends and regulation in the biotechnology industry, among others. Be
    10 KB (1,692 words) - 19:05, 28 July 2015
  • 20.109(F08): Colony PCR
    ...rlVersion=0&_userid=501045&md5=b1c5e4e2f9e0094a16d7b6ac47413f7f associated review article] that was written to celebrate and highlight this important work on
    9 KB (1,467 words) - 19:05, 28 July 2015
  • 20.109(F08): Screen for phenotypes, isolate RNA
    Many phenotypes have been described for <i> S. cerevisiae</i>. A very fine review of these was published by [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db ...dopt=AbstractPlus&list_uids=9301019&query_hl=2&itool=pubmed_docsum Hampsey review] to familiarize yourself with the range of phenotypes that are detectable a
    21 KB (3,401 words) - 19:05, 28 July 2015
  • 20.109(F08): old announcements
    ...ue to the religious holiday and would like to meet on Friday (10.10.08) to review the material we covered, please just email me and we can set up a time. [[U
    3 KB (565 words) - 19:05, 28 July 2015
  • 20.109(F08): Mod 3 Day 1 Growth of phage materials
    ...earch tool for finding peptides that interact with materials to meet human needs. That M13 could interact with inorganic materials could not have been predi ... bio-material engineering. During lab next time, you and your partner will review the topics and narrow your choices, identifying one or perhaps two topics f
    16 KB (2,756 words) - 19:05, 28 July 2015
  • 20.109(F08):DNA engineering/DNA engineering by PCR (Day 1)
    ...basic information on homologous recombination, please obtain the excellent review by Thomas Helleday from the References section of the [http://openwetware.o
    23 KB (3,892 words) - 19:30, 28 July 2015
  • 20.109(F08):DNA engineering/Restriction map and tissue culture (Day 6)
    ... minutes) and the gel will be photographed. While you are waiting, you can review and discuss the assigned article.
    11 KB (1,738 words) - 19:05, 28 July 2015
  • 20.109(F08): Growth of phage materials
    ...earch tool for finding peptides that interact with materials to meet human needs. Phage that can bind to cobalt oxide, gold, iridium and indium tin oxide ar ... bio-material engineering. During lab next time, you and your partner will review the topics and narrow your choices, identifying one or perhaps two topics f
    10 KB (1,707 words) - 19:05, 28 July 2015
  • 20.109(F08): Mod 2 Day 4 SDS-PAGE, screen for phenotypes
    Many phenotypes have been described for <i> S. cerevisiae</i>. A very fine review of these was published by [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db ...dopt=AbstractPlus&list_uids=9301019&query_hl=2&itool=pubmed_docsum Hampsey review] to familiarize yourself with the range of phenotypes that are detectable a
    25 KB (4,068 words) - 19:05, 28 July 2015
  • 20.109(F08): Mod 1 Day 1 DNA engineering using PCR
    ...basic information on homologous recombination, please obtain the excellent review by Thomas Helleday from the References section of the [http://openwetware.o
    22 KB (3,807 words) - 19:29, 28 July 2015
  • 20.109(F08): Mod 1 Day 6 Restriction map and tissue culture
    ... minutes) and the gel will be photographed. While you are waiting, you can review and discuss the assigned article.
    11 KB (1,701 words) - 19:05, 28 July 2015
  • 20.109(F08): Mod 1 Day 7 Lipofection
    ...re)familiarize yourself with these mechanisms by (re)reading the excellent review by Thomas Helleday that you can find in the References section of the [http
    10 KB (1,701 words) - 19:05, 28 July 2015
  • 20.109(F08): Mod 2 Day 2 Yeast transformation
    ...distinguish these possible explanations but it can tell you that something needs to be re-worked. ...s important work on the structure of the SAGA complex. You might find this review helpful but you are not required to read it. <br>
    18 KB (3,152 words) - 19:06, 28 July 2015
  • 20.109(F08): Mod 2 Day 3 Colony PCR and journal article discussion
    ...csum Wu et al, published in Mol Cell in 2004]. There is also an associated review article [http://openwetware.org/wiki/PMID:_15653319 ] that was written to c
    9 KB (1,612 words) - 19:06, 28 July 2015
  • 20.109(F08):Guidelines for writing up your research
    ...e way a journal’s editor decides whether to send your paper out for peer review or reject it as uninteresting and not generally relevant. Consequently, a w
    12 KB (1,922 words) - 19:06, 28 July 2015
  • 20.109(F08):Guidelines for oral presentations
    *Review each of your main messages
    5 KB (761 words) - 19:06, 28 July 2015
  • 20.109(S09):Guidelines for oral presentations
    *Review each of your main messages
    5 KB (788 words) - 19:34, 28 July 2015
  • 20.109(S09):Guidelines for writing up your research
    ...e way a journals editor decides whether to send your paper out for peer review or reject it as uninteresting and not generally relevant. Consequently, a w
    13 KB (2,033 words) - 19:34, 28 July 2015
  • 20.109(F09): Mod 2 Day 5 Assessing re-tuned system
    ...light and dark to assess the β-gal activity associated with your mutants. Review the [[20.109(F09): Mod 2 Day 2 Measuring system performance| protocol]] tha
    17 KB (2,716 words) - 19:39, 28 July 2015
  • 20.109(S09):Site-directed mutagenesis (Day2)
    ...ool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum this review by David Clapham in Cell], or [http://www.ncbi.nlm.nih.gov/pubmed/11830654?
    13 KB (2,155 words) - 19:33, 28 July 2015
  • 20.109(S09):Data analysis (Day8)
    ...nable titration curves by following the example of Nagai, the introduction/review of binding constants below may help contextualize your analysis.
    14 KB (2,303 words) - 19:33, 28 July 2015
  • 20.109(S09): siRNA design (Day1)
    ...ssion next time. You will have 45 minutes of in-class time to complete and review your reading. As before, each pair of students will present one figure or s
    22 KB (3,613 words) - 19:33, 28 July 2015
  • 20.109(S09):Wrap-up analysis (Day7)
    <i>As you can see from this image, taken from a review on hydrocarbon metabolism in marine bacteria, the alcanivorax species is th
    5 KB (904 words) - 19:33, 28 July 2015
  • 20.109(F09): Fall 2009 schedule
    | Neal/Linda visit lab for peer review exercise<br> [[Media:20.109(F09) M1D6lab.pptx| NK lab notes]], [[Media:F09_
    9 KB (1,307 words) - 19:42, 28 July 2015
  • 20.109(F09): Mod 1 Day 1 DNA engineering using PCR
    ...basic information on homologous recombination, please obtain the excellent review by Thomas Helleday from the References section of the [http://openwetware.o
    22 KB (3,783 words) - 19:44, 28 July 2015
  • 20.109(F09): Mod 3 Day 4 Battery assembly
    ...should be well on your way to your final presentations of a research idea. Review the required elements for your presentation that are noted [http://openwetw
    5 KB (921 words) - 19:38, 28 July 2015
  • 20.109(F09): Mod 1 Day 4 DNA ligations and bacterial transformations
    ...t 2 copies of this draft for next time. We will use them in lab for a peer review exercise. * Background and Significance: A concise (i.e. '''one paragraph''') review of the scientific premise that is relevant to the proposal, with emphasis o
    10 KB (1,761 words) - 19:38, 28 July 2015
  • 20.109(F09): Mod 1 Day 5 Examine candidate clones
    ...are too harsh or too obscure to be integrated into a revision. During your review, find at least one good example in the background/aims/results/plans/figure
    11 KB (1,830 words) - 19:38, 28 July 2015
  • 20.109(F09): Mod 1 Day 6 Restriction map and tissue culture
    ...l will be photographed. While the gel is running, we will engage in a peer review session around the preliminary results sections that you've all written as
    11 KB (1,830 words) - 19:38, 28 July 2015
  • 20.109(F09): Mod 1 Day 7 Lipofection
    ...re)familiarize yourself with these mechanisms by (re)reading the excellent review by Thomas Helleday that you can find in the References section of the [http
    10 KB (1,594 words) - 19:38, 28 July 2015
  • 20.109(F09):DNA engineering "Progress Report"
    * Background and Significance: A concise (i.e. '''one paragraph''') review of the scientific premise that is relevant to the proposal, with emphasis o ... be used to detect homologous recombination in mammalian cells. The reader needs to know what the goal is: which plasmid did you set out to create? Part B o
    7 KB (1,083 words) - 19:38, 28 July 2015
  • 20.109(F09):Guidelines for oral presentations
    *Review each of your main messages
    5 KB (777 words) - 19:38, 28 July 2015
  • 20.109(F09):Guidelines for writing up your research
    ...e way a journal’s editor decides whether to send your paper out for peer review or reject it as uninteresting and not generally relevant. Consequently, a w
    13 KB (1,951 words) - 19:38, 28 July 2015
  • 20.109(F09): Mod 3 Day 6 Oral presentations
    <i>As you can see from this image, taken from a review on hydrocarbon metabolism in marine bacteria, the alcanivorax species is th
    5 KB (879 words) - 19:38, 28 July 2015
  • 20.109(F09):Lab tour
    # Review todays lab to prepare for the lab practical that you and your partner wi
    27 KB (4,508 words) - 19:38, 28 July 2015
  • 20.109(F09): Mod 2 Day 6 Readouts of DNA, Protein
    Review [[20.109(F09): Mod 2 Day 1 Testing an engineered biological system| protoco
    13 KB (2,115 words) - 19:38, 28 July 2015
  • 20.109(F09): Mod 3 Day 1 Growth of phage materials
    ...earch tool for finding peptides that interact with materials to meet human needs. That M13 could interact with inorganic materials could not have been predi ... bio-material engineering. During lab next time, you and your partner will review the topics and narrow your choices, identifying one or perhaps two topics f
    16 KB (2,666 words) - 19:38, 28 July 2015
  • 20.109(S10):Wrap-up analysis (Day7)
    <i>As you can see from this image, taken from a review on hydrocarbon metabolism in marine bacteria, the alcanivorax species is th
    5 KB (919 words) - 19:49, 28 July 2015
  • 20.109(F10): Mod 2 Day 7 Readout of Protein
    ...ummary statement will be due at the end of the next module so you can also review the requirements for this assignment [http://openwetware.org/wiki/20.109(F1
    10 KB (1,613 words) - 21:00, 28 July 2015
  • 20.109(F10):DNA engineering "Progress Report"
    * Background and Significance: A concise (i.e. '''one paragraph''') review of the scientific premise that is relevant to the proposal, with emphasis o ... be used to detect homologous recombination in mammalian cells. The reader needs to know what the goal is: which plasmid did you set out to create? Part B o
    7 KB (1,131 words) - 21:00, 28 July 2015
  • 20.109(F10):Lab tour
    # Review todays lab to prepare for the lab practical that you and your partner wi
    27 KB (4,538 words) - 21:00, 28 July 2015
  • 20.109(F10):Guidelines for writing up your research
    ...e way a journal’s editor decides whether to send your paper out for peer review or reject it as uninteresting and not generally relevant.
    18 KB (2,793 words) - 21:00, 28 July 2015
  • 20.109(F10):Guidelines for oral presentations
    *Review each of your main messages
    5 KB (815 words) - 21:00, 28 July 2015
  • 20.109(F10): Fall 2010 schedule
    ...10) M1D6 labtalk.pdf| M1D6 lab lecture]]<br> Neal/Linda visit lab for peer review exercise<br>
    8 KB (1,189 words) - 21:00, 28 July 2015
  • 20.109(F10): Mod 1 Day 1 DNA engineering using PCR
    ...basic information on homologous recombination, please obtain the excellent review by Thomas Helleday from the References section of the [http://openwetware.o
    22 KB (3,783 words) - 21:00, 28 July 2015
  • 20.109(F10): Mod 3 Day 6 Oral presentations
    <i>As you can see from this image, taken from a review on hydrocarbon metabolism in marine bacteria, the alcanivorax species is th
    5 KB (880 words) - 21:00, 28 July 2015
  • 20.109(F10): Mod 1 Day 4 DNA ligations and bacterial transformations
    ...t 2 copies of this draft for next time. We will use them in lab for a peer review exercise. * Background and Significance: A concise (i.e. '''one paragraph''') review of the scientific premise that is relevant to the proposal, with emphasis o
    11 KB (1,791 words) - 21:00, 28 July 2015
  • 20.109(F10): Mod 1 Day 5 Examine candidate clones
    ...are too harsh or too obscure to be integrated into a revision. During your review, find at least one good example in the background/aims/results/plans/figure
    11 KB (1,830 words) - 21:00, 28 July 2015
  • 20.109(S11):RNA to DNA by RT-PCR (Day5)
    ...lve patient intake of heme to inhibit the defective synthetic pathway (see review [http://www.ncbi.nlm.nih.gov/pubmed/19479318 at this link]).
    8 KB (1,243 words) - 21:05, 28 July 2015
  • 20.109(S11):Prepare DNA for cloning (Day3)
    ...s include those that regulate chemotaxis, osmoregulation, and virulence. A review of 2CS from the perspective of appropriate signal transduction &mdash; e.g.
    17 KB (2,967 words) - 21:05, 28 July 2015
  • 20.109(F10): Fall 2011 schedule
    | ? peer review exercise, or maybe lab practical on part 1<br>
    5 KB (682 words) - 21:07, 28 July 2015
  • 20.109(F11): Mod 1 Day 1 DNA engineering using PCR
    ...basic information on homologous recombination, please obtain the excellent review by Thomas Helleday from the References section of the [http://openwetware.o
    22 KB (3,809 words) - 21:08, 28 July 2015
  • 20.109(F11):DNA engineering powerpoint summary
    *a review of the science on which the recombination assay is based
    3 KB (528 words) - 21:08, 28 July 2015
  • 20.109(F11): Mod 3 Day 6 Oral presentations
    <i>As you can see from this image, taken from a review on hydrocarbon metabolism in marine bacteria, the alcanivorax species is th
    5 KB (879 words) - 21:08, 28 July 2015

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