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- 20:42, 20 November 2007 (diff | hist) . . (+34) . . 20.109(S08):Module 2
- 20:40, 20 November 2007 (diff | hist) . . (+157) . . 20.109(S08):Module 2
- 16:33, 12 October 2007 (diff | hist) . . (+77) . . 20.109(S08):Module 2
- 03:59, 12 October 2007 (diff | hist) . . (+135) . . 20.109(S08):Module 2
- 00:58, 11 October 2007 (diff | hist) . . (+190) . . 20.109(F07): Module 2 oral presentations
- 00:45, 11 October 2007 (diff | hist) . . (+3) . . 20.109(F07): Luciferase assays and RNA prep (→Facts vs Data...plus a measure of measurement)
- 00:44, 11 October 2007 (diff | hist) . . (-1) . . 20.109(F07): Luciferase assays and RNA prep (→For next time)
- 00:43, 11 October 2007 (diff | hist) . . (+11) . . 20.109(F07): Luciferase assays and RNA prep (→Part 3: Isolation of total RNA)
- 00:34, 11 October 2007 (diff | hist) . . (0) . . 20.109(F07): Transfection (→For next time)
- 21:59, 10 October 2007 (diff | hist) . . (+96) . . 20.109(S08):Module 2
- 21:58, 10 October 2007 (diff | hist) . . (+1,156) . . N 20.109(S08):Module 2 (New page: Module 2 Day 1: Start-up protein engineering<br> (Initial reading about CaM-M13? Get calcium titration curve for WT plasmid? Need this day?...)
- 20:54, 10 October 2007 (diff | hist) . . (+717) . . 20.109(S08): Spring 2008 schedule
- 20:50, 10 October 2007 (diff | hist) . . (0) . . 20.109(S08):Module 1
- 20:49, 10 October 2007 (diff | hist) . . (0) . . 20.109(S08):Module 1
- 20:45, 10 October 2007 (diff | hist) . . (0) . . 20.109(S08):Module 1
- 17:30, 10 October 2007 (diff | hist) . . (-1) . . 20.109(S08):DNA engineering/Lab report (→Schedule for Module 1 laboratory report)
- 17:29, 10 October 2007 (diff | hist) . . (+41) . . 20.109(S08):DNA engineering/Lab report (→Schedule for Module 1 laboratory report)
- 17:28, 10 October 2007 (diff | hist) . . (+10,141) . . N 20.109(S08):DNA engineering/Lab report (New page: {{Template:20.109(S08)}} ==Schedule for Module 1 laboratory report== FIX DATES/NAMES!!! ===March 16/17, 2006=== *First draft is due by 10:00 a.m. on either the 16th or the 17th, dependi...)
- 17:27, 10 October 2007 (diff | hist) . . (+4,109) . . N 20.109(S08):DNA engineering/FACS analysis (Day 8) (New page: {{Template:20.109(S08)}} ==Introduction== FACS stands for "Fluorescence Activated Cell Sorting." The FACS machine has revolutionized biology by allowing researchers to isolate cells bas...)
- 17:27, 10 October 2007 (diff | hist) . . (+8,290) . . N 20.109(S08):DNA engineering/Lipofection (Day 7) (New page: {{Template:20.109(S08)}} ==Introduction== DNA can be put into mammalian cells in a process called transfection. If you wanted to make a mouse cell fluoresce green, you could transfect it...)
- 17:26, 10 October 2007 (diff | hist) . . (+9,853) . . N 20.109(S08):DNA engineering/Restriction map and tissue culture (Day 6) (New page: {{Template:20.109(S08)}} ==Introduction== In the past century, we have learned a tremendous amount by studying the behavior of mammalian cells maintained in the laboratory. Tissue cultur...)
- 17:25, 10 October 2007 (diff | hist) . . (+9,424) . . N 20.109(S08):DNA engineering/Examine candidate clones (Day 5) (New page: {{Template:20.109(S08)}} ==Introduction== Yesterday a few milliliters of LB+AMP broth were inoculated with some candidate colonies and the tubes were grown overnight at 37°C. Ampici...)
- 17:25, 10 October 2007 (diff | hist) . . (+10,010) . . N 20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4) (New page: {{Template:20.109(S08)}} ==Introduction== Today you will ligate your linearized plasmid backbone with your PCR product by mixing the two in the presence of ATP and an enzyme, T4 DNA liga...)
- 17:24, 10 October 2007 (diff | hist) . . (+10,685) . . N 20.109(S08):DNA engineering/Agarose gel electrophoresis (Day 3) (New page: {{Template:20.109(S08)}} ==Introduction== Electrophoresis is a technique that separates large molecules by size using an applied electrical field and a sieving matrix. DNA, RNA and prote...)
- 17:23, 10 October 2007 (diff | hist) . . (+9,151) . . N 20.109(S08):DNA engineering/Clean and cut DNA (Day 2) (New page: {{Template:20.109(S08)}} ==Introduction== Restriction endonucleases, also called restriction enzymes, cut (“digest”) DNA at specific sequences of bases. The restriction enzymes are n...)
- 17:22, 10 October 2007 (diff | hist) . . (+26,240) . . N 20.109(S08):DNA engineering/DNA engineering by PCR (Day 1) (New page: {{Template:20.109(S08)}} ==Introduction== As you heard about in lecture, you’ll be starting a project to study homologous recombination and in the process you’ll be learning some fun...)
- 17:21, 10 October 2007 (diff | hist) . . (+26) . . 20.109(S08):Module 1
- 17:20, 10 October 2007 (diff | hist) . . (+45) . . 20.109(S08):Module 1 (→Lab handouts)
- 17:19, 10 October 2007 (diff | hist) . . (+20) . . 20.109(S08):Module 1 (→Module 1)
- 17:18, 10 October 2007 (diff | hist) . . (-2) . . 20.109(S08):Module 1 (→Module 1)
- 17:18, 10 October 2007 (diff | hist) . . (+3,713) . . N 20.109(S08):Module 1 (New page: <div style="padding: 10px; width: 720px; border: 5px solid #2D9258;"> ==Module 1== '''Instructors:''' [http://web.mit.edu/be/people/engelward.htm Bevin Engelward] and [[Natalie Kuldell]...)
- 16:33, 3 October 2007 (diff | hist) . . (+190) . . 20.109(F07): Module 1 oral presentations
- 17:43, 5 September 2007 (diff | hist) . . (-1) . . 20.109(F07): siRNA design (→Part 1: siRNA design)
- 19:01, 4 September 2007 (diff | hist) . . (-8) . . 20.109(F07): siRNA design (→Part 1: siRNA design)
- 17:23, 4 September 2007 (diff | hist) . . (+1) . . 20.109(F07): Western analysis (→Part 2: Plaque assay)
- 17:22, 4 September 2007 (diff | hist) . . (-1) . . 20.109(F07): Western analysis (→Part 1: SDS-PAGE)
- 15:54, 4 September 2007 (diff | hist) . . (-1) . . 20.109(F07): M13.1 (→Reagents list)
- 15:53, 4 September 2007 (diff | hist) . . (-1) . . 20.109(F07): M13.1 (→Part 3: Sequencing)
- 15:51, 4 September 2007 (diff | hist) . . (+1) . . 20.109(F07): M13.1 (→Step 3: identify flanking restriction sites)
- 15:50, 4 September 2007 (diff | hist) . . (-1) . . 20.109(F07): M13.1 (→RBS)
- 15:49, 4 September 2007 (diff | hist) . . (+3) . . 20.109(F07): M13.1 (→Step 1: identify parts)
- 15:47, 4 September 2007 (diff | hist) . . (-1) . . 20.109(F07): M13.1 (→Step 1: identify parts)
- 15:25, 4 September 2007 (diff | hist) . . (-2) . . 20.109(F07): DNA ligation and bacterial transformation (→For next time)
- 15:24, 4 September 2007 (diff | hist) . . (+22) . . 20.109(F07): DNA ligation and bacterial transformation (→For next time)
- 15:21, 4 September 2007 (diff | hist) . . (0) . . 20.109(F07): DNA ligation and bacterial transformation (→For next time)
- 15:30, 20 August 2007 (diff | hist) . . (+175) . . 20.109(S08): Spring 2008 schedule
- 15:29, 20 August 2007 (diff | hist) . . (+5) . . 20.109(S08): Spring 2008 schedule
- 15:28, 20 August 2007 (diff | hist) . . (+20) . . 20.109(S08): Spring 2008 schedule
- 15:27, 20 August 2007 (diff | hist) . . (+57) . . 20.109(S08): Spring 2008 schedule
- 20:17, 17 August 2007 (diff | hist) . . (-18) . . 20.109(S08)
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