QRT-PCR

From Course Wiki
Revision as of 19:36, 17 May 2011 by Kristin Kuhn (Talk | contribs)

Jump to: navigation, search

Or to Get Started?

Pcr-setup.jpg
20.345: QRT-PCR

Current State

taken 5/8/11

Optics

Auto-fluorescence with the fiber optic is still a problem. Although one option is to keep looking for a fiber with sufficiently low auto-fluorescence levels, it may be more worthwhile to redesign the optics setup so that a fiber optic is no longer needed.

If this latter route is taken, top-down reading (i.e., reading the fluorescence through the cap of the PCR tube) will likely be the best way to avoid unwanted noise from the illuminator.


Electronics

LabView

Go to LabView QRT-PCR version info page

Going Forward

Recent Iterations

Go to Spring 2011 Progress Details.

Resources

Research Notes

Useful External Links