QRT-PCR:Get Started
From Course Wiki
Posted by Kristin Kuhn 19:03, 4 May 2011 (EDT)
This page is designed to be useful for whoever is just starting to work on the 20.309/345 QRT-PCR machine.
Sorry, no info on the optics (yet).
Contents
The Basics
The Circuitry
The Code
How to Actually Cycle the Thing
Hook it up
1. Make sure the lid is off. 2. Put in a non-empty PCR tube. 3. Screw the lid on. Make sure the wires don't get tangled. Be especially careful with the thermistors. 4. The lid heater thermistor hooks into the red and black grabber clips. 5. The resistance wire plugs into the female clip-on ends.
Turn it on
1. Open LabView. (As of 19:03, 4 May 2011 (EDT), 2.712 is the best). Turn on the circuit's power supply. 2. Make sure the lid heater current is set to 0.00 A. 3. Turn on the fan/lid heater power supply. 4. Start the VI. 5. Turn on the TEC power supply. 6.
