Difference between revisions of "Final Project -- Nathan S Lachenmyer"
From Course Wiki
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* Control software | * Control software | ||
* Getting good resolution | * Getting good resolution | ||
| + | |||
| + | =Weekly Updates= | ||
| + | ==2012 April 8== | ||
| + | * Look for PMTs and/or Galvos | ||
| + | |||
| + | ==2012 April 15== | ||
| + | * Calculations for stuff | ||
| + | |||
| + | ==2012 April 22== | ||
| + | * Construct Galvos, Program Maple | ||
| + | |||
| + | ==2012 April 29== | ||
| + | * Write-up for Fluorescence Microscope | ||
| + | |||
| + | ==2012 May 06== | ||
| + | * | ||
Latest revision as of 06:53, 7 May 2012
Contents
Confocal Microscopy
Literature
- Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth
- Confocal microscopy for biologists / Alan R. Hibbs.
- Handbook of biological confocal microscopy / editor, James B. Pawley.
Goals and Checkpoints
Goals
| Key Factors | Proposed Technical Targets | Requirements for Success | Current Practice | Associated Technical Barriers |
|---|---|---|---|---|
| OCLSM Cost | < $8,000 | < $15,000 | > $100,000 commercial; ~$30,000 DIY | Cheap replacement for PMT & Galvo, etc. |
| OCLSM Scan Time | 1 frame/second | 1 frame / 10 seconds | 10-50 fps | Fast scanning mirrors + ADC time |
Timeline
- 2 Weeks: Brightfield Confocal Microscopy
- 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images
- 3 Weeks: Laser Scanning Features
Proposed Design
Challenges
- Control software
- Getting good resolution
Weekly Updates
2012 April 8
- Look for PMTs and/or Galvos
2012 April 15
- Calculations for stuff
2012 April 22
- Construct Galvos, Program Maple
2012 April 29
- Write-up for Fluorescence Microscope
