Difference between revisions of "Final Project -- Nathan S Lachenmyer"

From Course Wiki
Jump to: navigation, search
Line 1: Line 1:
−
I have two project proposals that I am deciding between:
+
{| border = "1" align="center" style="color: black; background-color: #ffffcc;" width="85%" class="wikitable"
 +
!Key Factors!!Proposed Technical Targets!!Requirements for Success!!Current Practice!!Associated Technical Barriers
 +
 
 +
|- align="center"
 +
|style="width: 20%;"|OCLSM Cost
 +
|style="width: 20%;"|< $8,000
 +
|style="width: 20%;"|< $15,000
 +
|style="width: 20%;"|> $100,000 commercial; ~$30,000 DIY
 +
|style="width: 20%;"|Cheap replacement for PMT & Galvo, etc.
 +
|-
 +
|style="width: 20%;"|OCLSM Scan Time
 +
|style="width: 20%;"|1 frame/second
 +
|style="width: 20%;"|1 frame / 10 seconds
 +
|style="width: 20%;"|10-50 fps
 +
|style="width: 20%;"|Fast scanning mirrors + ADC time
 +
|}
  
 
=Confocal Microscopy=
 
=Confocal Microscopy=

Revision as of 16:02, 21 March 2012

Key Factors Proposed Technical Targets Requirements for Success Current Practice Associated Technical Barriers
OCLSM Cost < $8,000 < $15,000 > $100,000 commercial; ~$30,000 DIY Cheap replacement for PMT & Galvo, etc.
OCLSM Scan Time 1 frame/second 1 frame / 10 seconds 10-50 fps Fast scanning mirrors + ADC time

Confocal Microscopy

Literature

  • Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth
  • Confocal microscopy for biologists / Alan R. Hibbs.
  • Handbook of biological confocal microscopy / editor, James B. Pawley.

Goals and Checkpoints

  • 2 Weeks: Brightfield Confocal Microscopy
  • 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images
  • 3 Weeks: Laser Scanning Features

Proposed Design

Challenges

  • Control software
  • Getting good resolution
Retrieved from "http://measurebiology.org/w/index.php?title=Final_Project_--_Nathan_S_Lachenmyer&oldid=5335"