Difference between revisions of "Final Project -- Nathan S Lachenmyer"

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* Control software
 
* Control software
 
* Getting good resolution
 
* Getting good resolution
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=Ca<math>^{2+}</math> Imaging=
 
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==Literature==
 
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* Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth
 
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* Handbook of biological confocal microscopy / editor, James B. Pawley.
 
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* Detection and Measurement of Free Ca2+ in Cells / editor, Ashley Campbell
 
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* Calcium Measurement Methods / editors, Alexei Verkhratsky and Ole Peterson
 
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==Goals and Checkpoints==
 
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* 1 Weeks: Fluorescence Microscopy Setup (with UV)
 
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* 2 Weeks: Software Image Capture, PSF Characterized
 
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* 2 Weeks: Switching between UV Sources implemented, Image Analysis Done
 
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* 2 Weeks: [Ca] samples characterized
 
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==Proposed Design==
 
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* Fluorescence Microscope
 
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** 422 nm (brightfield, optical)
 
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** 340, 380 nm (fluorescence; two wavelengths provides ratiometric determination of [Ca])
 
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* Motorized Mirror or Filter (Servo) to switch wavelengths
 
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* Sync motor signal with camera signal (take pictures)
 
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* Post-Processing Analysis to determine [Ca](t)
 
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==Challenges==
 
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* Syncing up motor with camera for fluorescence imaging
 
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* Acquiring UV Optics
 
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* Biggest Risk: Biology doesn't work out
 

Revision as of 04:45, 21 March 2012

I have two project proposals that I am deciding between:

Confocal Microscopy

Literature

  • Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth
  • Confocal microscopy for biologists / Alan R. Hibbs.
  • Handbook of biological confocal microscopy / editor, James B. Pawley.

Goals and Checkpoints

  • 2 Weeks: Brightfield Confocal Microscopy
  • 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images
  • 3 Weeks: Laser Scanning Features

Proposed Design

Challenges

  • Control software
  • Getting good resolution
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