Difference between revisions of "Final Project -- Nathan S Lachenmyer"
From Course Wiki
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* Handbook of biological confocal microscopy / editor, James B. Pawley. | * Handbook of biological confocal microscopy / editor, James B. Pawley. | ||
| − | == | + | ==Goals and Checkpoints== |
| − | * | + | * 2 Weeks: Brightfield Confocal Microscopy |
| − | * | + | * 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images |
| − | + | * 3 Weeks: Laser Scanning Features | |
| − | * | + | |
==Proposed Design== | ==Proposed Design== | ||
| − | |||
| − | |||
==Challenges== | ==Challenges== | ||
| + | |||
=Ca<math>^{2+}</math> Imaging= | =Ca<math>^{2+}</math> Imaging= | ||
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* Calcium Measurement Methods / editors, Alexei Verkhratsky and Ole Peterson | * Calcium Measurement Methods / editors, Alexei Verkhratsky and Ole Peterson | ||
| − | ==Goals== | + | ==Goals and Checkpoints== |
| − | * | + | * 1 Weeks: Fluorescence Microscopy Setup (with UV) |
| − | * | + | * 2 Weeks: Software Image Capture, PSF Characterized |
| − | * | + | * 2 Weeks: Switching between UV Sources implemented, Image Analysis Done |
| − | * | + | * 2 Weeks: [Ca] samples characterized |
==Proposed Design== | ==Proposed Design== | ||
Revision as of 17:00, 14 March 2012
I have two project proposals that I am deciding between:
Contents
Confocal Microscopy
Literature
- Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth
- Confocal microscopy for biologists / Alan R. Hibbs.
- Handbook of biological confocal microscopy / editor, James B. Pawley.
Goals and Checkpoints
- 2 Weeks: Brightfield Confocal Microscopy
- 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images
- 3 Weeks: Laser Scanning Features
Proposed Design
Challenges
Ca$ ^{2+} $ Imaging
Literature
- Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth
- Handbook of biological confocal microscopy / editor, James B. Pawley.
- Detection and Measurement of Free Ca2+ in Cells / editor, Ashley Campbell
- Calcium Measurement Methods / editors, Alexei Verkhratsky and Ole Peterson
Goals and Checkpoints
- 1 Weeks: Fluorescence Microscopy Setup (with UV)
- 2 Weeks: Software Image Capture, PSF Characterized
- 2 Weeks: Switching between UV Sources implemented, Image Analysis Done
- 2 Weeks: [Ca] samples characterized
Proposed Design
- Fluorescence Microscope
- 422 nm (brightfield, optical)
- 340, 380 nm (fluorescence; two wavelengths provides ratiometric determination of [Ca])
- Motorized Mirror or Filter (Servo) to switch wavelengths
- Sync motor signal with camera signal (take pictures)
- Post-Processing Analysis to determine [Ca](t)
Challenges
- Syncing up motor with camera for fluorescence imaging
