Difference between revisions of "Final Project -- Nathan S Lachenmyer"
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| + | =Confocal Microscopy= | ||
| + | ==Literature== | ||
| + | * Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth | ||
| + | * Confocal microscopy for biologists / Alan R. Hibbs. | ||
| + | * Handbook of biological confocal microscopy / editor, James B. Pawley. | ||
| + | |||
| + | ==Goals and Checkpoints== | ||
| + | ===Goals=== | ||
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!Key Factors!!Proposed Technical Targets!!Requirements for Success!!Current Practice!!Associated Technical Barriers | !Key Factors!!Proposed Technical Targets!!Requirements for Success!!Current Practice!!Associated Technical Barriers | ||
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| − | = | + | ===Timeline=== |
| − | == | + | |
| − | + | ||
| − | + | ||
| − | + | ||
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* 2 Weeks: Brightfield Confocal Microscopy | * 2 Weeks: Brightfield Confocal Microscopy | ||
* 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images | * 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images | ||
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==Proposed Design== | ==Proposed Design== | ||
| + | |||
==Challenges== | ==Challenges== | ||
* Control software | * Control software | ||
* Getting good resolution | * Getting good resolution | ||
| + | |||
| + | =Weekly Updates= | ||
| + | ==2012 April 8== | ||
| + | * Look for PMTs and/or Galvos | ||
| + | |||
| + | ==2012 April 15== | ||
| + | * Calculations for stuff | ||
| + | |||
| + | ==2012 April 22== | ||
| + | * Construct Galvos, Program Maple | ||
| + | |||
| + | ==2012 April 29== | ||
| + | * Write-up for Fluorescence Microscope | ||
| + | |||
| + | ==2012 May 06== | ||
| + | * | ||
Latest revision as of 06:53, 7 May 2012
Contents
Confocal Microscopy
Literature
- Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth
- Confocal microscopy for biologists / Alan R. Hibbs.
- Handbook of biological confocal microscopy / editor, James B. Pawley.
Goals and Checkpoints
Goals
| Key Factors | Proposed Technical Targets | Requirements for Success | Current Practice | Associated Technical Barriers |
|---|---|---|---|---|
| OCLSM Cost | < $8,000 | < $15,000 | > $100,000 commercial; ~$30,000 DIY | Cheap replacement for PMT & Galvo, etc. |
| OCLSM Scan Time | 1 frame/second | 1 frame / 10 seconds | 10-50 fps | Fast scanning mirrors + ADC time |
Timeline
- 2 Weeks: Brightfield Confocal Microscopy
- 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images
- 3 Weeks: Laser Scanning Features
Proposed Design
Challenges
- Control software
- Getting good resolution
Weekly Updates
2012 April 8
- Look for PMTs and/or Galvos
2012 April 15
- Calculations for stuff
2012 April 22
- Construct Galvos, Program Maple
2012 April 29
- Write-up for Fluorescence Microscope
