Latest revision as of 06:53, 7 May 2012

Confocal Microscopy

Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth
Confocal microscopy for biologists / Alan R. Hibbs.
Handbook of biological confocal microscopy / editor, James B. Pawley.

Key Factors	Proposed Technical Targets	Requirements for Success	Current Practice	Associated Technical Barriers
OCLSM Cost	< $8,000	< $15,000	> $100,000 commercial; ~$30,000 DIY	Cheap replacement for PMT & Galvo, etc.
OCLSM Scan Time	1 frame/second	1 frame / 10 seconds	10-50 fps	Fast scanning mirrors + ADC time

@@ Line 1: / Line 1: @@
-I have two project proposals that I am deciding between:
 =Confocal Microscopy=
 ==Literature==
@@ Line 8: / Line 6: @@
 ==Goals and Checkpoints==
+===Goals===
+{| border = "1" align="center" style="color: black; background-color: #ffffcc;" width="85%" class="wikitable"
+!Key Factors!!Proposed Technical Targets!!Requirements for Success!!Current Practice!!Associated Technical Barriers
+|- align="center"
+|style="width: 20%;"|OCLSM Cost
+|style="width: 20%;"|< $8,000
+|style="width: 20%;"|< $15,000
+|style="width: 20%;"|> $100,000 commercial; ~$30,000 DIY
+|style="width: 20%;"|Cheap replacement for PMT & Galvo, etc.
+|-
+|style="width: 20%;"|OCLSM Scan Time
+|style="width: 20%;"|1 frame/second
+|style="width: 20%;"|1 frame / 10 seconds
+|style="width: 20%;"|10-50 fps
+|style="width: 20%;"|Fast scanning mirrors + ADC time
+|}
+===Timeline===
 * 2 Weeks: Brightfield Confocal Microscopy
 * 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images
@@ Line 13: / Line 30: @@
 ==Proposed Design==
 ==Challenges==
 * Control software
 * Getting good resolution
+=Weekly Updates=
+==2012 April 8==
+* Look for PMTs and/or Galvos
+==2012 April 15==
+* Calculations for stuff
+==2012 April 22==
+* Construct Galvos, Program Maple
+==2012 April 29==
+* Write-up for Fluorescence Microscope
+==2012 May 06==
+*