Difference between revisions of "Final Project -- Nathan S Lachenmyer"
From Course Wiki
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+ | =Confocal Microscopy= | ||
+ | ==Literature== | ||
+ | * Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth | ||
+ | * Confocal microscopy for biologists / Alan R. Hibbs. | ||
+ | * Handbook of biological confocal microscopy / editor, James B. Pawley. | ||
+ | |||
+ | ==Goals and Checkpoints== | ||
+ | ===Goals=== | ||
{| border = "1" align="center" style="color: black; background-color: #ffffcc;" width="85%" class="wikitable" | {| border = "1" align="center" style="color: black; background-color: #ffffcc;" width="85%" class="wikitable" | ||
!Key Factors!!Proposed Technical Targets!!Requirements for Success!!Current Practice!!Associated Technical Barriers | !Key Factors!!Proposed Technical Targets!!Requirements for Success!!Current Practice!!Associated Technical Barriers | ||
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− | = | + | ===Timeline=== |
− | == | + | |
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− | + | ||
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* 2 Weeks: Brightfield Confocal Microscopy | * 2 Weeks: Brightfield Confocal Microscopy | ||
* 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images | * 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images | ||
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==Proposed Design== | ==Proposed Design== | ||
+ | |||
==Challenges== | ==Challenges== | ||
* Control software | * Control software | ||
* Getting good resolution | * Getting good resolution |
Revision as of 16:02, 21 March 2012
Contents
Confocal Microscopy
Literature
- Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth
- Confocal microscopy for biologists / Alan R. Hibbs.
- Handbook of biological confocal microscopy / editor, James B. Pawley.
Goals and Checkpoints
Goals
Key Factors | Proposed Technical Targets | Requirements for Success | Current Practice | Associated Technical Barriers |
---|---|---|---|---|
OCLSM Cost | < $8,000 | < $15,000 | > $100,000 commercial; ~$30,000 DIY | Cheap replacement for PMT & Galvo, etc. |
OCLSM Scan Time | 1 frame/second | 1 frame / 10 seconds | 10-50 fps | Fast scanning mirrors + ADC time |
Timeline
- 2 Weeks: Brightfield Confocal Microscopy
- 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images
- 3 Weeks: Laser Scanning Features
Proposed Design
Challenges
- Control software
- Getting good resolution