20.109(S19):Class data

Module 1: Assessing ligand binding

M1D3: SMM analysis

Files for SMM analysis

GAL files linked here.

Scan images and set numbers assigned to each team:

Team	Slide #1	Slide #2
T/R
Red	14399983, set 1	14400077, set 4
Orange	14400113, set 2	14400139, set 3
Yellow	14400114, set 2	14400140, set 3
Green	14399984, set 5	14399988, set 6
Blue	14400072, set 5	14399987, set 6
Pink	14399983, set 1	14400128, set 7
Purple	14400077, set 4	14399959, set 8
White	14400129, set 7	14400081, set 9
Grey	14400128, set 7	14399959, set 8
W/F
Red	14400129, set 7	14400081, set 9
Orange	14400128, set 7	14399959, set 8
Yellow	14400143, set 11	14399950, set 12
Green	14400147, set 10	14399951, set 12
Blue	14400146, set 10	14399947, set 11
Pink	14399983, set 1	14400077, set 4
Grey	14400113, set 2	14400139, set 3

Ligand selections

Team	Ligand #1	Ligand #2
T/R
Red	31064975	13742946
Orange	83759472	34120495
Yellow	34297777	64183606
Green	85836506	41507070
Blue	71743840	64472564
Pink	47464042	85836506
Purple	47464042	63365116
White	62716075	45527639
Grey	62716075	64472564
W/F
Red	41507070	13742946
Orange	34297777	64183606
Yellow	45714400	31064975
Green	34120495	71743840
Blue	56421147	45527639
Pink	63365116	45714400
Grey	41507070	65421147

M1D3: Evaluate protein purity and concentration

WF Section

BCA Assay results (A₅₆₂ measurements): linked here

SDS-PAGE images:
Red Team
Orange Team
Yellow Team
Green Team
Blue Team
Pink Team
Grey Team

M1D5: PPIase assay

TR Section

Plate #1: [[Media: | linked here]]
Plate #2: [[Media: | linked here]]

WF Section

Plate #1: linked here
Plate #2: linked here
Plate #3: linked here

Module 2: Measuring gene expression

==Module 3: Engineering biomaterials==