Difference between revisions of "20.109(S07): Agarose gel electrophoresis"
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[[Image:Be109agarosegelsetup.jpg|thumb|left|500px]] | [[Image:Be109agarosegelsetup.jpg|thumb|left|500px]] | ||
− | Today you will separate DNA fragments using an agarose matrix. Agarose is a polymer that comes from seaweed and if | + | Today you will separate DNA fragments using an agarose matrix. Agarose is a polymer that comes from seaweed and if you ve ever made Jell-O , then you already have all the skills for pouring an agarose gel. To prepare these gels, agarose and buffer are microwaved until the agarose is melted. The molten agar is then poured into a horizontal casting tray, and a comb is added. Once the agar has solidified, the comb is removed, leaving wells into which the DNA sample can be loaded. |
− | The distance a DNA fragment travels is inversely proportional to its length. Over time fragments of similar length accumulate into | + | [[Image:Be109gelpercentage.jpg|right|thumb|150px]] |
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+ | The distance a DNA fragment travels is inversely proportional to its length. Over time fragments of similar length accumulate into |