20.109(F19): Prep notes for M3
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M3D1
24 hr preceding laboratory:
- seed MEF (250K) in T25 flasks, 1 per team
- cell expansion schedule for D1 and D2 prep
in tissue culture room (per team):
- 1 fresh T75 flask
- 3.5 mL PBS
- 1.5 mL trypsin
- 15 mL media
- not aliquoted: trypan blue
in main lab (per team):
- none
M3D2
48 hr preceding laboratory:
- seed MEF (2-3M) in T75 flasks, 4 per team
in tissue culture room (per team):
- 25 mL PBS
- 45 mL media
- not aliquoted: ligands
in main lab (per team):
- 80 mL PBS
- 8.5 mL PBS
- 5 mL trypsin
- 15 mL media
- not aliquoted: protease inhibitor, dry ice
