Difference between revisions of "20.109(F19):Module 2"
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M2D3: [[20.109(F19):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br> | M2D3: [[20.109(F19):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br> | ||
M2D4: [[20.109(F19):Journal club presentation (Day4 and 6)| Journal club I]]<br> | M2D4: [[20.109(F19):Journal club presentation (Day4 and 6)| Journal club I]]<br> | ||
| − | M2D5: [[20.109(F19):Confirm gRNA sequence in pgRNA(Day5)| Confirm gRNA sequence in pgRNA]]<br> | + | M2D5: [[20.109(F19):Confirm gRNA sequence in pgRNA (Day5)| Confirm gRNA sequence in pgRNA]]<br> |
M2D6: [[20.109(F19):Journal club presentation (Day4 and 6) | Journal club II]]<br> | M2D6: [[20.109(F19):Journal club presentation (Day4 and 6) | Journal club II]]<br> | ||
M2D7: [[20.109(F19):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br> | M2D7: [[20.109(F19):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br> | ||
Latest revision as of 19:12, 14 August 2019
Contents
Module 2
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Becky Meyer
TAs: Colin Kim and Shelbi Parker
Lab manager: Hsinhwa Lee
Overview
In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of E. coli such that either ethanol or acetate production is increased.
Lab links: day by day
M2D1: Complete in silico cloning of pdCas9
M2D2: Design gRNA for CRISPRi
M2D3: Generate gRNA plasmid
M2D4: Journal club I
M2D5: Confirm gRNA sequence in pgRNA
M2D6: Journal club II
M2D7: Induce CRISPRi system
M2D8: Measure fermentation products
Assignments
Research article
Journal club presentation
References
CRISPR interference (CRISPRi) for sequence-specific control of gene expression., Nature Methods. (2013) 8: 2180-2196.
