20.109(F19):Class data

Module 1: Measuring genomic instability

M1D5

gamma-H2AX analysis

Team	Upload, then link the completed excel spreadsheet from the wiki here!
TR Orange	Orange Team Data
TR Yellow	Yellow Team Data
TR Green	Green Team Data
TR Blue	Blue Team Data
TR Pink	Pink Team Data
TR Purple	Purple Team Data
WF Cyan	WF Team Data

M1D6

Comet Chip analysis

Team
TR Orange	Orange Team Data
TR Yellow	Yellow Team Data
TR Green	Green Team Data
TR Blue	Blue Team Data
TR Pink	Pink Team Data
TR Purple	Purple Team Data
WF Cyan	WF Team Data

Fermentation product and gene targeted:

T/R

Team	Ethanol (E) or Acetate (A)	Gene targeted by CRISPRi gRNA	gRNA (DNA) sequence (without tag at 3' end)	Locus targeted (eg. beginning of gene, putative promoter, -35 region)	Target template or nontemplate strand	Colorimetric Assay Results
TR orange	E	ldhA	GTACTGTTTTGTGCTATAAA	Coding region	Non-template	[[File: | Raw data ]]
TR yellow	E	ldhA	AAATTCCAGCTCAAAGCCAAAGG	Coding region	Non-template	M2ColorimetricAssay_Template.xlsx ]
TR green	E	ack	TTAGCCACGTATCAATTATAGG	Promoter Region	Template	[[[Media: M2ColorimetricAssay_Template.xlsx|Yellow Team Data]]]
TR blue	A	adhE	ccagagcggcggcgcggaag	Coding region	Non-template	[[File: | Raw data ]]
TR pink	E	ppc	AGCATACTGACATTACTACGCAATG	Coding region	Non-Template	[[File: | Raw data ]]
TR purple	E	ldhA	CTTAAATGTGATTCAACATCACTGG	Promoter region	Template	[[File: | Raw data ]]

W/F

Team	Ethanol (E) or Acetate (A)	Gene targeted by CRISPRi gRNA	gRNA (DNA) sequence (without tag at 3' end)	Locus targeted (eg. beginning of gene, putative promoter, -35 region)	Target template or nontemplate strand	Colorimetric Assay Results
WF Cyan	E	frd	GTGGGATAAAAACAATCTGG	promoter	Template	[[File: | Raw data ]]
WF Cyan	E	ppc	ACTGACATTACTACGCAATG	beginning of gene	Non-template	[[File: | Raw data ]]
WF Cyan	E	pta	TTTGTAACCCGCCAAATCGG	promoter	Template	[[File: | Raw data ]]