Difference between revisions of "20.109(F19):Class data"
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Latest revision as of 19:04, 3 December 2019
Contents
Module 1: Measuring genomic instability
M1D5
gamma-H2AX analysis
| Team | Upload, then link the completed excel spreadsheet from the wiki here! |
| TR Orange | Orange Team Data |
| TR Yellow | Yellow Team Data |
| TR Green | Green Team Data |
| TR Blue | Blue Team Data |
| TR Pink | Pink Team Data |
| TR Purple | Purple Team Data |
| WF Cyan | WF Team Data |
M1D6
Comet Chip analysis
| Team | |
| TR Orange | Orange Team Data |
| TR Yellow | Yellow Team Data |
| TR Green | Green Team Data |
| TR Blue | Blue Team Data |
| TR Pink | Pink Team Data |
| TR Purple | Purple Team Data |
| WF Cyan | WF Team Data |
Fermentation product and gene targeted:
T/R
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA (DNA) sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target template or nontemplate strand | Colorimetric Assay Results |
| TR orange | E | ldhA | GTACTGTTTTGTGCTATAAA | Coding region | Non-template | Orange Team Data |
| TR yellow | E | ldhA | AAATTCCAGCTCAAAGCCAAAGG | Coding region | Non-template | Yellow Team Data |
| TR green | E | ack | TTAGCCACGTATCAATTATAGG | Promoter Region | Template | Green Team Data |
| TR blue | A | adhE | ccagagcggcggcgcggaag | Coding region | Non-template | Blue Team data |
| TR pink | E | ppc | AGCATACTGACATTACTACGCAATG | Coding region | Non-Template | Pink Team data |
| TR purple | E | ldhA | CTTAAATGTGATTCAACATCACTGG | Promoter region | Template | Purple Team Data |
W/F
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA (DNA) sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target template or nontemplate strand | Colorimetric Assay Results |
| WF Cyan | E | frd | GTGGGATAAAAACAATCTGG | promoter | Template | WF FRD DATA ATHENA |
| WF Cyan | A | ppc | ACTGACATTACTACGCAATG | beginning of gene | Non-template | WF PPC Data |
| WF Cyan | E | pta | TTTGTAACCCGCCAAATCGG | promoter | Template | WF pta data |
