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| | R/F Feb 6/7 | | | R/F Feb 6/7 |
| | [http://be.mit.edu/directory/angela-koehler AK]<br> | | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
− | | [[20.109(S20):In silico cloning and induction of TDP43 protein expression (Day1) |In silico cloning and induction of TDP43 protein expression]]<br> | + | | [[20.109(S20):Complete in silico cloning and induce TDP43 protein expression (Day1) |Complete in silico cloning and induce TDP43 protein expression]]<br> |
| | <font color= #01A9DBF>'''Orientation quiz'''</font color> <br> [[20.109(S20):Homework#Due_M1D1| Homework due]] | | | <font color= #01A9DBF>'''Orientation quiz'''</font color> <br> [[20.109(S20):Homework#Due_M1D1| Homework due]] |
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| | R/F Feb 13/14 | | | R/F Feb 13/14 |
| | [http://be.mit.edu/directory/angela-koehler AK]<br> | | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
− | | [[20.109(S20):Analyze purity and concentration of TDP43 protein (Day3) | Analyze purity and concentration of TDP43 protein]]<br> | + | | [[20.109(S20):Assess purity and concentration of TDP43 protein (Day3) | Assess purity and concentration of TDP43 protein]]<br> |
| | [[20.109(S20):Homework#Due_M1D3| Homework due]] | | | [[20.109(S20):Homework#Due_M1D3| Homework due]] |
| |-- | | |-- |
Revision as of 20:22, 16 January 2020
20.109(S20): Laboratory Fundamentals of Biological Engineering
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm (56-154)
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm (56-322)
MODULE
|
DAY
|
DATE
|
LECTURER
|
LABORATORY EXPERIMENTS
|
ASSIGNMENTS
|
|
|
T/W Feb 4/5
|
NLL
|
Orientation and laboratory tour
|
|
|
1
|
1
|
R/F Feb 6/7
|
AK
|
Complete in silico cloning and induce TDP43 protein expression
|
Orientation quiz Homework due
|
1
|
2
|
T/W Feb 11/12
|
AK
|
Purify TDP43 protein
|
Homework due
|
1
|
3
|
R/F Feb 13/14
|
AK
|
Assess purity and concentration of TDP43 protein
|
Homework due
|
|
|
T/W Feb 18/19
|
|
President's day holiday
|
|
1
|
4
|
R/F Feb 20/21
|
AK
|
Perform small molecule microarray (SMM) with TDP43 protein
|
Laboratory quiz Homework due
|
1
|
5
|
T/W Feb 25/26
|
AK
|
Scan SMM slides to identify binders of TDP43
|
Homework due
|
1
|
6
|
R/F Feb 27/28
|
AK
|
Analyze SMM data
|
Homework due
|
1
|
7
|
T/W Mar 3/4
|
AK
|
Examine TDP43 binders for chemical features
|
Laboratory quiz Homework due
|
|
2
|
1
|
R/F Mar 5/6
|
NLL
|
Practice tissue culture techniques and prepare cells for RNA purification
|
Homework due
|
2
|
2
|
T/W Mar 10/11
|
EF
|
Etoposide treat cells for RNA purification
|
Homework due Data summary draft due Mon, Mar 11 at 10 pm
|
2
|
3
|
R/F Mar 12/13
|
EF
|
Purify RNA and practice RNA-seq data analysis methods
|
Homework due Mini-presentation due Sat, Mar 16 at 10 pm [https:// Blog post due] Sun, Mar 17 at 10 pm
|
2
|
4
|
T/W Mar 17/18
|
EF
|
Analyze RNA-seq data and prepare for quantitative PCR experiment
|
Laboratory quiz Homework due
|
2
|
5
|
R/F Mar 19/20
|
EF
|
Perform quantitative PCR experiment and explore additional RNA-seq datasets
|
Homework due
|
|
|
T/W Mar 24/25
|
|
Spring vacation
|
Data summary revision due Mon, Mar 25 at 10 pm
|
|
|
R/F Mar 26/27
|
|
Spring vacation
|
|
2
|
6
|
T/W Mar/Apr 31/1
|
EF
|
Design cell viability experiment and complete RNA-seq data analysis
|
Homework due
|
2
|
7
|
R/F Apr 2/3
|
EF
|
Journal club I presentations
|
[https:// Blog post due] Sat, Apr 6 at 10 pm
|
2
|
8
|
T/W Apr 7/8
|
NLL
|
Journal club II presentations
|
|
|
3
|
1
|
R/F Apr 9/10
|
LM
|
Complete data analysis
|
Laboratory quiz Homework due
|
3
|
2
|
R/F Apr 14/15
|
Comm Lab
|
Grow phage-based active (cathode) material
|
Homework due Research article due Sat, Apr 20 at 10 pm [https:// Blog post due] Sun, Apr 21 at 10 pm
|
3
|
3
|
T/W Apr 16/17
|
LM
|
Purify phage-based active (cathode) material
|
Homework due
|
|
|
T/W Apr 21/22
|
|
Patriots' day holiday
|
|
3
|
4
|
T/W Apr 23/24
|
LM
|
Construct cathode with phage-based active material
|
Laboratory quiz Homework due
|
|
|
T/W Apr 28/29
|
|
Research proposal pitches in lecture, no laboratory
|
|
3
|
5
|
R/F Apr/May 30/1
|
LM
|
Visualize phage nanowires using transmission electron microscopy
|
Homework due
|
3
|
6
|
T/W May 5/6
|
LM
|
Assemble and test batteries
|
Laboratory quiz Homework due
|
|
|
R/F May 7/8
|
|
Research proposal presentations
|
[https:// Blog post due] Sat, May 11 at 10 pm
|
|
|
T May 12
|
|
Feedback and celebratory lunch
|
Mini-report due Mon, May 13 at 10 pm
|