Difference between revisions of "Final Project -- Nathan S Lachenmyer"
From Course Wiki
(Created page with "I have two project proposals that I am deciding between: =Confocal Microscopy= ==Literature== * Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yu...") |
(→Confocal Microscopy) |
||
Line 13: | Line 13: | ||
** Laser Scanning for CLSM | ** Laser Scanning for CLSM | ||
− | == | + | ==Proposed Design== |
+ | |||
+ | ==Challenges== | ||
=Ca<math>^{2+}</math> Imaging= | =Ca<math>^{2+}</math> Imaging= |
Revision as of 00:54, 13 March 2012
I have two project proposals that I am deciding between:
Contents
Confocal Microscopy
Literature
- Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth
- Confocal microscopy for biologists / Alan R. Hibbs.
- Handbook of biological confocal microscopy / editor, James B. Pawley.
Goal
- Minimum: Create a z-stack of images of a 3D sample
- Potential Improvements:
- 3D Reconstruction in Software
- Laser Scanning for CLSM
Proposed Design
Challenges
Ca$ ^{2+} $ Imaging
Literature
- Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth
- Handbook of biological confocal microscopy / editor, James B. Pawley.
- Detection and Measurement of Free Ca2+ in Cells / editor, Ashley Campbell
- Calcium Measurement Methods / editors, Alexei Verkhratsky and Ole Peterson
Goals
- Determine Ca$ ^{2+} $ concentration in a biological system
- Potential Improvements:
- Lifetime vs. Intensity Measurements
- FRET Measurements
=Challenges
- Syncing up motor with camera for fluorescence imaging