Difference between revisions of "20.109(F19): Fall 2019 schedule"
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| 5 | | 5 | ||
| R/F Oct 24/25 | | R/F Oct 24/25 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa19 M2D5 lecture slides v2.pdf| Lecture slides]] |
− | | [[20.109(F19):Confirm gRNA sequence in pgRNA (Day5)| Confirm gRNA sequence in pgRNA]]<br> | + | | [[20.109(F19):Confirm gRNA sequence in pgRNA (Day5)| Confirm gRNA sequence in pgRNA]]<br> [[Media: M2D5_Fa19_LMmarked.pdf | TR Prelab]]<br> [[Media:Fa19 M2D5 cyk marked.pdf | WF Prelab]] |
| <font color=#298A08>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M2D5|Homework due]]<br>[[20.109(F19): Data Summary| Data Summary revision due]] Sat, Oct 26 at 10 pm | | <font color=#298A08>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M2D5|Homework due]]<br>[[20.109(F19): Data Summary| Data Summary revision due]] Sat, Oct 26 at 10 pm | ||
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| 6 | | 6 | ||
| T/W Oct 29/30 | | T/W Oct 29/30 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa19 M2D6 lecture slides.pdf|Lecture slides]] |
| [[20.109(F19):Journal club presentation (Day4 and 6) | Journal club II]] | | [[20.109(F19):Journal club presentation (Day4 and 6) | Journal club II]] | ||
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| 7 | | 7 | ||
| R/F Oct/Nov 31/1 | | R/F Oct/Nov 31/1 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa19 M2D7 lecture slides.pdf| Lecture slides]] |
− | | [[20.109(F19):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br> | + | | [[20.109(F19):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br> [[Media: M2D7_Fa19_LM.pdf | TR Prelab]] <br> [[Media:Fa18 M2D7 bcm marked.pdf | WF Prelab]] |
| [[20.109(F19):Homework#Due_M2D7|Homework due]]<br>[https://be20109f19.blogspot.com/ Blog post due] Fri, Nov 1 at 10 pm | | [[20.109(F19):Homework#Due_M2D7|Homework due]]<br>[https://be20109f19.blogspot.com/ Blog post due] Fri, Nov 1 at 10 pm | ||
|-- | |-- | ||
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| T/W Nov 5/6 | | T/W Nov 5/6 | ||
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | ||
− | | [[20.109(F19):Measure_fermentation_products_(Day8) | Measure fermentation products]]<br> | + | | [[20.109(F19):Measure_fermentation_products_(Day8) | Measure fermentation products]]<br> [[Media: M2D8_Fa19_LMmarked.pdf | TR Prelab]] <br> [[Media:Fa19 M2D8 bcm marked.pdf | WF Prelab]] |
| <font color=#298A08>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M2D8|Homework due]] | | <font color=#298A08>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M2D8|Homework due]] | ||
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| 1 | | 1 | ||
| R/F Nov 7/8 | | R/F Nov 7/8 | ||
− | | [https://be.mit.edu/directory/angela-koehler AK]<br> | + | | [https://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M3L1 2019.pptx|Lecture slides]] |
− | | [[20.109(F19):Prepare for cellular thermal shift assay (Day1)| Prepare for cellular thermal shift assay]]<br> | + | | [[20.109(F19):Prepare for cellular thermal shift assay (Day1)| Prepare for cellular thermal shift assay]]<br> [[Media:Fa19 M3D1 prelab slides nllmarked.pdf| TR Prelab]] <br> [[Media:Fa19 M3D1 bcm marked.pdf | WF Prelab]] |
| [[20.109(F19):Homework#Due_M3D1|Homework due]] <br> | | [[20.109(F19):Homework#Due_M3D1|Homework due]] <br> | ||
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| 2 | | 2 | ||
| R/F Nov 14/15 | | R/F Nov 14/15 | ||
− | | [https://be.mit.edu/directory/angela-koehler AK]<br> | + | | [https://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M3L2 2019.pptx|Lecture slides]] |
− | | [[20.109(F19):Incubate with ligand and apply heat treatment for protein denaturation (Day2)| Incubate with ligand and apply heat treatment for protein denaturation]]<br> | + | | [[20.109(F19):Incubate with ligand and apply heat treatment for protein denaturation (Day2)| Incubate with ligand and apply heat treatment for protein denaturation]]<br>[[Media:Fa19 M3D2 prelab slides nllmarked.pdf|TR Prelab]] <br> [[Media:191115 prelab CYK marked.pdf | WF Prelab]] |
| [[20.109(F19):Homework#Due_M3D2|Homework due]]<br> | | [[20.109(F19):Homework#Due_M3D2|Homework due]]<br> | ||
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| 3 | | 3 | ||
| T/W Nov 19/20 | | T/W Nov 19/20 | ||
− | | [https://be.mit.edu/directory/angela-koehler AK]<br> | + | | [https://be.mit.edu/directory/angela-koehler AK]<br> [[Media:20109 Struntz Lecture 2019.pdf| Lecture slides]] |
− | | [[20.109(F19):Begin Western blot analysis (Day3)| Begin Western blot analysis]]<br> | + | | [[20.109(F19):Begin Western blot analysis (Day3)| Begin Western blot analysis]]<br>[[Media:Fa19 M3D3 prelab slides nllmarked.pdf|TR Prelab]] <br> [[Media:Fa19 M3D3 bcm.pdf | WF Prelab]] |
| <font color=#298A08>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M3D3| Homework due]] | | <font color=#298A08>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M3D3| Homework due]] | ||
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| 4 | | 4 | ||
| R/F Nov 21/22 | | R/F Nov 21/22 | ||
− | | [https://be.mit.edu/directory/angela-koehler AK]<br> | + | | [https://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M3L4 2019.pdf| Lecture slides]] |
− | | [[20.109(F19):Complete antibody staining for Western blot analysis (Day4)| Complete antibody staining for Western blot analysis]]<br> | + | | [[20.109(F19):Complete antibody staining for Western blot analysis (Day4)| Complete antibody staining for Western blot analysis]]<br> [[Media:M3D4 Fa19 bcm marked.pdf | TR Prelab]] <br> [[Media:M3D4WF Fa19 bcm marked.pdf | WF Prelab]] |
| [[20.109(F19):Homework#Due_M3D4|Homework due]] | | [[20.109(F19):Homework#Due_M3D4|Homework due]] | ||
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| 5 | | 5 | ||
| T/W Dec 3/4 | | T/W Dec 3/4 | ||
− | | [https://be.mit.edu/directory/angela-koehler AK]<br> | + | | [https://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M3L5 2019.pdf| Lecture slides]] |
− | | [[20.109(F19):Data analysis and assignment preparation (Day5)| Data analysis and assignment preparation]]<br> | + | | [[20.109(F19):Data analysis and assignment preparation (Day5)| Data analysis and assignment preparation]]<br>[[Media:Fa19 M3D5 prelab slides nllmarked.pdf| TR Prelab]] <br> [[Media:Fa19 M3D5 bcm marked.pdf | WF Prelab]] |
| <font color=#298A08>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M3D5|Homework due]] | | <font color=#298A08>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M3D5|Homework due]] | ||
|-- | |-- |
Latest revision as of 19:19, 4 December 2019
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm (16-220)
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm (56-322)