Difference between revisions of "20.109(F08): Fall 2008 schedule"

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| Tue Sept 9  
 
| Tue Sept 9  
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
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| [[20.109(F07):Start-up genome engineering | Start-up genome engineering]]
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| [[20.109(F08):Start-up genome engineering | DNA engineering using PCR]]
 
|  
 
|  
 
|--
 
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| Thur Sept 11   
 
| Thur Sept 11   
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
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| [[20.109(F07): Agarose gel electrophoresis | Agarose gel electrophoresis]]
+
| [[20.109(F08): Agarose gel electrophoresis | Clean and cut DNA]]
 
|  
 
|  
 
|--
 
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| Tue Sept 16  
 
| Tue Sept 16  
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F07): DNA ligation and bacterial transformation| DNA ligation and bacterial transformation]]
+
| [[20.109(F08): DNA ligation and bacterial transformation| Agarose gel electrophoresis]]
 
|  
 
|  
 
|--
 
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| Thur Sept 18  
 
| Thur Sept 18  
 
| [[http://web.mit.edu/be/people/engelward.htm BE]
 
| [[http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
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| [[20.109(F07): Examine candidate clones| Examine candidate clones]]
+
| [[20.109(F08): Examine candidate clones| DNA ligation and bacterial transformation]]
 
|  
 
|  
 
|--
 
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| Tue Sept 23  
 
| Tue Sept 23  
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F07): M13.1| M13.1]]
+
| [[20.109(F08): M13.1| Examine candidate clones]]
 
|  
 
|  
 
|--
 
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| Thur Sept 25  
 
| Thur Sept 25  
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F07): Western analysis| Western analysis]]
+
| [[20.109(F08): Western analysis| Restriction map and tissue culture]]
 
|  
 
|  
 
|--
 
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| Tue Sept 30  
 
| Tue Sept 30  
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F07): Probe western| Probe western]]
+
| [[20.109(F08): Probe western| Lipofection]]
 
|  
 
|  
 
|--
 
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| Thur Oct 2  
 
| Thur Oct 2  
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F07): Module 1 oral presentations| Oral Presentations]]
+
| [[20.109(F08): Module 1 oral presentations| FACS analysis]]
 
|  
 
|  
 
|--
 
|--
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| Tues Oct 7
 
| Tues Oct 7
 
| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
−
|  
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F07): siRNA design| siRNA design and start-up in cell culture]]
+
| [[20.109(F08): siRNA design| Protein engineering with PCR]]
 
|  
 
|  
 
|--
 
|--
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| Thur Oct 9
 
| Thur Oct 9
 
| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
−
|  
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F07): Transfection| Transfection]]
+
| [[20.109(F08): Transfection| Yeast transformation]]
 
|  
 
|  
 
|--
 
|--
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| Tues Oct 14
 
| Tues Oct 14
 
| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
−
|
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F07): Luciferase assays and RNA prep| Luciferase assays and RNA prep]]
+
| [[20.109(F08): Luciferase assays and RNA prep| Colony PCR, Journal article discussion]]
 
|
 
|
 
|--
 
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| Thur Oct 16
 
| Thur Oct 16
 
| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
−
|  
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F07): Journal article discussion| Journal article discussion]]
+
| [[20.109(F08): Journal article discussion| SDS-PAGE, screen for phenotypes]]
 
| Guest lecture: [[User:Nlerner| NL]] from the writing program.  
 
| Guest lecture: [[User:Nlerner| NL]] from the writing program.  
 
|-
 
|-
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| Tues Oct 21
 
| Tues Oct 21
 
| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
−
|
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F07): cDNA synthesis and microarray| cDNA synthesis and microarray]]
+
| [[20.109(F08): cDNA synthesis and microarray| Probe Western, isolate RNA]]
 
|  
 
|  
 
|--
 
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| Thurs Oct 23
 
| Thurs Oct 23
 
| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
−
|  
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F07): Microarray data analysis| Microarray data analysis]]
+
| [[20.109(F08): Microarray data analysis| Journal Club I]]
 
|  
 
|  
 
|--
 
|--
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| Tues Oct 28  
 
| Tues Oct 28  
 
| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
−
|  
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| Lecture on high throughput technologies;[[20.109(F07): Mod 2 Day 7| '''no lab''']]
+
| [[20.109(F08): Mod 2 Day 7| cDNA synthesis and microarray]]
 
|  
 
|  
 
|--
 
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| Thurs Oct 30   
 
| Thurs Oct 30   
 
| [[Natalie Kuldell | NK]]
 
| [[Natalie Kuldell | NK]]
−
|  
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F07): Module 2 oral presentations| Oral presentations]]
+
| [[20.109(F08): Module 2 oral presentations| Microarray data analysis]]
 
|  
 
|  
 
|--
 
|--
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| Tues Nov 4
 
| Tues Nov 4
 
| [[User:Abelcher| AB]]
 
| [[User:Abelcher| AB]]
−
|
+
| [http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109(F08): Growth of phage materials| Growth of phage materials]]
+
| [[20.109(F08): Growth of phage materials| Phage growth]]
 
|  
 
|  
 
|--
 
|--
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| Tue Nov 6  
 
| Tue Nov 6  
 
| [[User:Abelcher| AB]]
 
| [[User:Abelcher| AB]]
−
|  
+
| [http://openwetware.org/wiki/Luis_Alvarez LA]
 
| [[20.109(F08): Phage nanowires| Phage nanowires]]
 
| [[20.109(F08): Phage nanowires| Phage nanowires]]
 
|  
 
|  
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| Thur Nov 13
 
| Thur Nov 13
 
| [[User:Abelcher| AB]]
 
| [[User:Abelcher| AB]]
−
|  
+
| [http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109(F08): Transmission electron microscopy| TEM]]
+
| [[20.109(F08): Transmission electron microscopy| Journal Club II]]
 
|  
 
|  
 
|--
 
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| Tue Nov 18   
 
| Tue Nov 18   
 
| [[User:Abelcher| AB]]
 
| [[User:Abelcher| AB]]
−
|  
+
| [http://openwetware.org/wiki/Luis_Alvarez LA]
 
| [[20.109(F08): Phage by design| Phage by design]]
 
| [[20.109(F08): Phage by design| Phage by design]]
 
|  
 
|  
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| Thur Nov 20
 
| Thur Nov 20
 
| [[User:Abelcher| AB]]
 
| [[User:Abelcher| AB]]
−
|  
+
| [http://openwetware.org/wiki/Luis_Alvarez LA]
 
| [[20.109(F08): Phage by design, pt2| Phage by design, pt 2]]
 
| [[20.109(F08): Phage by design, pt2| Phage by design, pt 2]]
 
|  
 
|  
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| Tue Dec 2  
 
| Tue Dec 2  
 
| [[User:Abelcher| AB]]
 
| [[User:Abelcher| AB]]
−
|  
+
| [http://openwetware.org/wiki/Luis_Alvarez LA]
 
| [[20.109(F08): ECD assembly| ECD assembly]]
 
| [[20.109(F08): ECD assembly| ECD assembly]]
 
|  
 
|  
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| Thur Dec 4  
 
| Thur Dec 4  
 
| [[User:Abelcher| AB]]
 
| [[User:Abelcher| AB]]
−
|
+
|[http://openwetware.org/wiki/Luis_Alvarez LA]
 
| [[20.109(F08): Module 3 oral presentations| Oral presentations]]
 
| [[20.109(F08): Module 3 oral presentations| Oral presentations]]
 
|  
 
|  

Revision as of 00:07, 24 July 2008


20.109(F08): Laboratory Fundamentals of Biological Engineering

20.109(F08)pic.jpg

Home        People        Schedule Fall 2008        Assignments        Lab Basics        OWW Basics       
DNA Engineering        Protein Engineering        Biomaterials Engineering              

MODULE DAY DATE LECTURER TA LAB note
Thur Sept 4 NK Orientation
1 1 Tue Sept 9 BE MS DNA engineering using PCR
1 2 Thur Sept 11 BE MS Clean and cut DNA
1 3 Tue Sept 16 BE MS Agarose gel electrophoresis
1 4 Thur Sept 18 [BE MS DNA ligation and bacterial transformation
1 5 Tue Sept 23 BE MS Examine candidate clones
1 6 Thur Sept 25 BE MS Restriction map and tissue culture
1 7 Tue Sept 30 BE MS Lipofection
1 8 Thur Oct 2 BE MS FACS analysis
2 1 Tues Oct 7 NK BB Protein engineering with PCR
2 2 Thur Oct 9 NK BB Yeast transformation
2 3 Tues Oct 14 NK BB Colony PCR, Journal article discussion
2 4 Thur Oct 16 NK BB SDS-PAGE, screen for phenotypes Guest lecture: NL from the writing program.
2 5 Tues Oct 21 NK BB Probe Western, isolate RNA
2 6 Thurs Oct 23 NK BB Journal Club I
2 7 Tues Oct 28 NK BB cDNA synthesis and microarray
2 8 Thurs Oct 30 NK BB Microarray data analysis
3 1 Tues Nov 4 AB LA Phage growth
3 2 Tue Nov 6 AB LA Phage nanowires
Veteran's Day Break Nov 10,11 No lab Tuesday and Wednesday
3 3 Thur Nov 13 AB LA Journal Club II
3 4 Tue Nov 18 AB LA Phage by design
3 5 Thur Nov 20 AB LA Phage by design, pt 2
Thanksgiving Break Nov No lab or lecture all week
3 6 Tue Dec 2 AB LA ECD assembly
3 7 Thur Dec 4 AB LA Oral presentations
3 8 Tue Dec 9 end of term celebration... Lecture, no lab, for class evaluations then lunch from noon-1:30 PM,
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