Talk:20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)
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Revision as of 16:56, 16 October 2015 by Erin Reynolds (Talk | contribs)
IPC mutants (and primers) chosen by teams
T/R RED
- mutation: D132H
- forward primer:
- reverse primer:
- restriction enzyme:
W/F RED
- mutation: D57H
- forward primer: GCC GGC AGT CGA TGC TCA TGG CAA TGG AA
- reverse primer: TC ATT GAT CAT ATC CTG CAA TTC
- restriction enzyme: NaeI - GCCGGC
T/R ORANGE
- mutation: T78P
- forward primer:
- reverse primer:
- restriction enzyme:
W/F YELLOW
- mutation: D21A
- forward primer: 5' GCC GGC ATT CGA CAA GGC TGG GGA CGG CA
- reverse primer: 5' AAT GAG AAG GCT TCT TTG AAC TCT GC
- restriction enzyme: NaeI - GCCGGC
T/R GREEN
- mutation: D57H
- forward primer:
- reverse primer:
- restriction enzyme:
W/F GREEN
- mutation: T78P
- forward primer: 5' CCA GGG AAT GAA GGA CCC AGA CAG CGA AG 3'
- reverse primer: 5' TTT CTA GCC ATC ATA GTA AGA AAT TCA GGA AAG TAA ATC GTT CC 3'
- restriction enzyme: StyI - C/CWWGG
T/R BLUE
- mutation: D132H
- forward primer: 5' TAT CGA TGG TCA TGG CCA AGT AAA C 3'
- reverse primer:
- restriction enzyme: None (no flap included)
W/F BLUE
- mutation: E30K
- forward primer: 5' CCA GGG CAC CAC AAA GAA ACT TGG CAC CG 3'
- reverse primer: 5' ATGGTGCCGTCCCCATCC 3'
- restriction enzyme: StyI-HF - C/CWWGG
T/R PINK
- mutation: D132H
- forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3'
- reverse primer: 5'TCT GCT TCC CTT ATC ATT TC 3'
- restriction enzyme: NaeI (GCC / GGC)
W/F PINK
- mutation: D132H
- forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3'
- reverse primer: 5'TCT GCT TCC CTT ATC ATT TC 3'
- restriction enzyme: NaeI (GCC / GGC)