User contributions
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- 18:32, 25 February 2022 (diff | hist) . . (-1) . . 20.109(S22):Spring 2022 schedule
- 20:14, 23 February 2022 (diff | hist) . . (+44) . . 20.109(S22):Spring 2022 schedule
- 20:14, 23 February 2022 (diff | hist) . . (0) . . N File:20109 M1L6 2022.pdf (current)
- 16:09, 16 February 2022 (diff | hist) . . (+44) . . 20.109(S22):Spring 2022 schedule
- 16:08, 16 February 2022 (diff | hist) . . (0) . . N File:20109 M1L5 2022.pdf (current)
- 15:23, 16 February 2022 (diff | hist) . . (+2) . . 20.109(S22):Communication (→Workshop Slides and Recordings)
- 15:23, 16 February 2022 (diff | hist) . . (+9) . . 20.109(S22):Communication (→Workshop Slides and Recordings)
- 15:22, 16 February 2022 (diff | hist) . . (0) . . N File:20.109 F22 Figures workshop.pdf (current)
- 16:22, 15 February 2022 (diff | hist) . . (+266) . . 20.109(S22):M1D4 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 21:35, 14 February 2022 (diff | hist) . . (+44) . . 20.109(S22):Spring 2022 schedule
- 21:34, 14 February 2022 (diff | hist) . . (0) . . N File:20109 M1L4 2022.pdf (current)
- 19:21, 14 February 2022 (diff | hist) . . (+54) . . 20.109(S22):M1D7 (→Reagents list)
- 19:21, 14 February 2022 (diff | hist) . . (+54) . . 20.109(S22):M1D5 (→Reagents list)
- 19:19, 14 February 2022 (diff | hist) . . (+35) . . 20.109(S22):M1D5 (→Reagents list)
- 19:18, 14 February 2022 (diff | hist) . . (0) . . 20.109(S22):M1D5 (→Part 3: Perform aggregation assay)
- 19:18, 14 February 2022 (diff | hist) . . (0) . . 20.109(S22):M1D5 (→Part 3: Perform aggregation assay)
- 19:17, 14 February 2022 (diff | hist) . . (+39) . . 20.109(S22):M1D5 (→Part 3: Perform aggregation assay)
- 19:16, 14 February 2022 (diff | hist) . . (0) . . N File:Sp22 M2D5 aggregation plate map.png (current)
- 19:05, 14 February 2022 (diff | hist) . . (+7) . . 20.109(S22):M1D5 (→Part 3: Perform aggregation assay)
- 18:56, 14 February 2022 (diff | hist) . . (+19) . . 20.109(S22):M1D5 (→Part 3: Perform aggregation assay)
- 18:55, 14 February 2022 (diff | hist) . . (+59) . . 20.109(S22):M1D5 (→Part 3: Perform aggregation assay)
- 18:55, 14 February 2022 (diff | hist) . . (+267) . . 20.109(S22):M1D5 (→Part 3: Perform aggregation assay)
- 18:47, 14 February 2022 (diff | hist) . . (+33) . . 20.109(S22):Class data (→W/F)
- 18:47, 14 February 2022 (diff | hist) . . (+23) . . 20.109(S22):Class data (→small molecule selections)
- 18:45, 14 February 2022 (diff | hist) . . (+61) . . 20.109(S22):M1D5 (→Part 3: Perform aggregation assay)
- 18:43, 14 February 2022 (diff | hist) . . (+791) . . 20.109(S22):M1D5 (→Part 3: Perform aggregation assay)
- 18:37, 14 February 2022 (diff | hist) . . (+3,225) . . 20.109(S22):M1D5 (→Part 3: Perform aggregation assay)
- 18:19, 14 February 2022 (diff | hist) . . (-3) . . 20.109(S22):M1D7 (→Part 1: Treat cells for TDP43-localization experiment)
- 18:18, 14 February 2022 (diff | hist) . . (+56) . . 20.109(S22):M1D7 (→Part 1: Treat cells for TDP43-localization experiment)
- 18:04, 14 February 2022 (diff | hist) . . (+6) . . 20.109(S22):M1D5 (→Part 2: Concentrate TDP43-RRM12 protein elution)
- 18:03, 14 February 2022 (diff | hist) . . (+1) . . 20.109(S22):M1D5 (→Part 2: Concentrate TDP43-RRM12 protein elution)
- 18:02, 14 February 2022 (diff | hist) . . (0) . . N File:Sp22 M1D5 protein concentration.png (current)
- 18:00, 14 February 2022 (diff | hist) . . (+138) . . 20.109(S22):M1D5 (→Part 2: Concentrate TDP43-RRM12 protein elution)
- 17:58, 14 February 2022 (diff | hist) . . (-1,095) . . 20.109(S22):M1D5 (→Part 2: Concentrate TDP43-RRM12 protein elution)
- 17:44, 14 February 2022 (diff | hist) . . (+754) . . 20.109(S22):M1D5 (→Introduction)
- 17:10, 14 February 2022 (diff | hist) . . (+10) . . 20.109(S22):M1D4 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 17:09, 14 February 2022 (diff | hist) . . (-202) . . 20.109(S22):M1D4 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 16:42, 14 February 2022 (diff | hist) . . (0) . . 20.109(S22):M1D4 (→Reagents list)
- 16:42, 14 February 2022 (diff | hist) . . (+54) . . 20.109(S22):M1D5 (→Reagents list)
- 16:42, 14 February 2022 (diff | hist) . . (-56) . . 20.109(S22):M1D4 (→Reagents list)
- 16:40, 14 February 2022 (diff | hist) . . (+2,718) . . 20.109(S22):M1D5 (→Protocols)
- 16:39, 14 February 2022 (diff | hist) . . (-1) . . 20.109(S22):M1D4 (→Protocols)
- 16:39, 14 February 2022 (diff | hist) . . (-2,715) . . 20.109(S22):M1D4 (→Protocols)
- 15:13, 14 February 2022 (diff | hist) . . (-2) . . 20.109(S22):M1D4 (→Part 4: Measure protein concentration)
- 21:41, 11 February 2022 (diff | hist) . . (0) . . 20.109(S22):Homework (→Due M2D6)
- 21:41, 11 February 2022 (diff | hist) . . (0) . . 20.109(S22):Homework (→Due M2D8)
- 21:41, 11 February 2022 (diff | hist) . . (0) . . 20.109(S22):Homework (→Due M2D7)
- 21:39, 11 February 2022 (diff | hist) . . (0) . . 20.109(S22):Homework (→Due M1D3)
- 20:01, 11 February 2022 (diff | hist) . . (+103) . . 20.109(S22):M1D4 (→Part 3: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 20:00, 11 February 2022 (diff | hist) . . (+41) . . 20.109(S22):M1D4 (→Part 3: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 19:52, 11 February 2022 (diff | hist) . . (+2) . . 20.109(S22):M1D3 (→Part 3: Desalt purified protein)
- 12:17, 11 February 2022 (diff | hist) . . (+9) . . 20.109(S22):M1D4 (→Part 2: Concentrate TDP43-RRM12 protein elution)
- 19:46, 10 February 2022 (diff | hist) . . (0) . . 20.109(S22):M1D3 (→Purify TDP43-RRM12 from cell lysate)
- 19:45, 10 February 2022 (diff | hist) . . (-71) . . 20.109(S22):M1D3 (→Purify TDP43-RRM12 from cell lysate)
- 19:44, 10 February 2022 (diff | hist) . . (0) . . 20.109(S22):M1D3 (→Purify TDP43-RRM12 from cell lysate)
- 19:44, 10 February 2022 (diff | hist) . . (-13) . . 20.109(S22):M1D3 (→Purify TDP43-RRM12 from cell lysate)
- 19:43, 10 February 2022 (diff | hist) . . (+1) . . 20.109(S22):M1D3 (→Purify TDP43-RRM12 from cell lysate)
- 16:03, 10 February 2022 (diff | hist) . . (+44) . . 20.109(S22):Spring 2022 schedule
- 16:03, 10 February 2022 (diff | hist) . . (0) . . N File:20109 M1L3 2022.pdf (current)
- 12:29, 9 February 2022 (diff | hist) . . (0) . . 20.109(S22):M1D3 (→Introduction)
- 18:48, 8 February 2022 (diff | hist) . . (+7) . . 20.109(S22):M1D3 (→Introduction)
- 18:10, 8 February 2022 (diff | hist) . . (+108) . . 20.109(S22):FYI (→Where and when to find help)
- 17:55, 8 February 2022 (diff | hist) . . (+387) . . 20.109(S22):M1D3 (→Lyse BL21(A1) cells expressing pET_MBP_SNAP_TDP43-RRM12)
- 17:39, 8 February 2022 (diff | hist) . . (+383) . . 20.109(S22):M1D3 (→Part 2: Purify TDP43-RRM12 protein)
- 16:46, 8 February 2022 (diff | hist) . . (+3) . . 20.109(S22):M1D3 (→Purify TDP43-RRM12 from cell lysate)
- 16:20, 8 February 2022 (diff | hist) . . (+15) . . 20.109(S22):M1D3 (→Purify TDP43-RRM12 from cell lysate)
- 16:19, 8 February 2022 (diff | hist) . . (-1) . . 20.109(S22):M1D3 (→Part 3: Desalt purified protein)
- 16:19, 8 February 2022 (diff | hist) . . (+9) . . 20.109(S22):M1D3 (→Part 3: Desalt purified protein)
- 16:19, 8 February 2022 (diff | hist) . . (+66) . . 20.109(S22):M1D3 (→Purify TDP43-RRM12 from cell lysate)
- 16:16, 8 February 2022 (diff | hist) . . (-89) . . 20.109(S22):M1D3 (→Purify TDP43-RRM12 from cell lysate)
- 16:15, 8 February 2022 (diff | hist) . . (-120) . . 20.109(S22):M1D3 (→Reagents list)
- 16:03, 8 February 2022 (diff | hist) . . (+26) . . 20.109(S22):M1D3 (→Reagents list)
- 16:00, 8 February 2022 (diff | hist) . . (+862) . . 20.109(S22):M1D3
- 15:53, 8 February 2022 (diff | hist) . . (-220) . . 20.109(S22):M1D3 (→Purify TDP43-RRM12 from cell lysate)
- 14:23, 8 February 2022 (diff | hist) . . (+50) . . 20.109(S22):Spring 2022 schedule
- 14:23, 8 February 2022 (diff | hist) . . (0) . . N File:20109 M1L2 2022 short.pdf (current)
- 18:51, 4 February 2022 (diff | hist) . . (0) . . 20.109(S22):Module 1 (→Major assignments) (current)
- 18:50, 4 February 2022 (diff | hist) . . (+9) . . 20.109(S22):Data Summary (→Visualizing TDP43-localation in CAD cells) (current)
- 18:49, 4 February 2022 (diff | hist) . . (+198) . . 20.109(S22):Data Summary (→Background and Motivation:)
- 18:45, 4 February 2022 (diff | hist) . . (-1) . . 20.109(S22):Assignments (→Major assignments)
- 18:44, 4 February 2022 (diff | hist) . . (0) . . 20.109(S22):Spring 2022 schedule
- 18:41, 4 February 2022 (diff | hist) . . (-106) . . 20.109(S22):FYI (→Where and when to find help)
- 18:41, 4 February 2022 (diff | hist) . . (+52) . . 20.109(S22):Assignments (→Contribution to Class blog)
- 18:40, 4 February 2022 (diff | hist) . . (+208) . . 20.109(S22):Spring 2022 schedule
- 18:31, 4 February 2022 (diff | hist) . . (+5) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results) (current)
- 18:31, 4 February 2022 (diff | hist) . . (+19) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 18:57, 3 February 2022 (diff | hist) . . (-23) . . 20.109(S22):M1D1 (→Perform SMM screen) (current)
- 15:27, 3 February 2022 (diff | hist) . . (+26) . . 20.109(S22):Spring 2022 schedule
- 15:27, 3 February 2022 (diff | hist) . . (0) . . N File:20109 M1L1 2022.pdf (current)
- 19:16, 2 February 2022 (diff | hist) . . (+43) . . 20.109(S22):Spring 2022 schedule
- 19:15, 2 February 2022 (diff | hist) . . (0) . . N File:Sp22 M0 nll.pdf (current)
- 12:33, 2 February 2022 (diff | hist) . . (+61) . . 20.109(S22):Spring 2022 schedule
- 12:32, 2 February 2022 (diff | hist) . . (0) . . N File:Sp22 Orientation lecture student.pptx (current)
- 00:29, 1 February 2022 (diff | hist) . . (+38) . . 20.109(S22):FYI (→How to contact the teaching team)
- 19:33, 27 January 2022 (diff | hist) . . (0) . . 20.109(S22):Class data (→W/F)
- 13:24, 26 January 2022 (diff | hist) . . (-6) . . 20.109(S22):M1D7 (→Complete primary staining steps)
- 13:24, 26 January 2022 (diff | hist) . . (-6) . . 20.109(S22):M1D8 (→Part 1: Image and analyze TDP43 localization experiment results)
- 14:51, 25 January 2022 (diff | hist) . . (+848) . . 20.109(S22):M2D6 (→Introduction)
- 14:39, 25 January 2022 (diff | hist) . . (+1) . . 20.109(S22):M2D6 (→Part 3: Analyze fermentation product yield data)
- 14:39, 25 January 2022 (diff | hist) . . (+52) . . 20.109(S22):M2D6 (→Part 3: Analyze fermentation product yield data)
- 14:38, 25 January 2022 (diff | hist) . . (0) . . 20.109(S22):M2D6 (→Part 3: Analyze fermentation product yield data)
- 14:36, 25 January 2022 (diff | hist) . . (+59) . . 20.109(S22):M2D6 (→Part 3: Analyze fermentation product yield data)
- 14:34, 25 January 2022 (diff | hist) . . (0) . . N File:Sp22 M2D6 data analysis template.xlsx (current)
- 13:15, 25 January 2022 (diff | hist) . . (-242) . . 20.109(S22):M2D6 (→Part 3: Analyze fermentation product yield data)
- 13:13, 25 January 2022 (diff | hist) . . (-55) . . 20.109(S22):M2D6 (→Part 3: Analyze fermentation product yield data)
- 13:12, 25 January 2022 (diff | hist) . . (+13) . . 20.109(S22):Class data (→gRNA sequences)
- 22:07, 24 January 2022 (diff | hist) . . (+67) . . 20.109(S22):M2D8 (→Exercises)
- 21:50, 24 January 2022 (diff | hist) . . (-5) . . 20.109(S22):M2D5 (→Part 1: Examine psgRNA_target sequencing results)
- 21:47, 24 January 2022 (diff | hist) . . (+72) . . 20.109(S22):M2D5 (→Reagents list)
- 21:46, 24 January 2022 (diff | hist) . . (+1) . . 20.109(S22):M2D5 (→Part 2:Prepare media for fermentation product yield experiment)
- 21:44, 24 January 2022 (diff | hist) . . (-11) . . 20.109(S22):M2D5 (→Part 1: Examine psgRNA_target sequencing results)
- 21:43, 24 January 2022 (diff | hist) . . (-2,409) . . 20.109(S22):M2D5 (→Part 4: Align sgRNA_target sequences to host genome)
- 21:40, 24 January 2022 (diff | hist) . . (-1) . . 20.109(S22):M2D5 (→Protocols)
- 21:40, 24 January 2022 (diff | hist) . . (+6) . . 20.109(S22):M2D5 (→Protocols)
- 21:39, 24 January 2022 (diff | hist) . . (+1,445) . . 20.109(S22):M2D5 (→Part 1: Examine psgRNA_target sequencing results)
- 21:27, 24 January 2022 (diff | hist) . . (+120) . . 20.109(S22):M2D5 (→Part 1: Examine psgRNA_target sequencing results)
- 21:26, 24 January 2022 (diff | hist) . . (+210) . . 20.109(S22):M2D5 (→Part 1: Examine psgRNA_target sequencing results)
- 21:20, 24 January 2022 (diff | hist) . . (+7) . . 20.109(S22):M2D5 (→Part 1: Examine psgRNA_target sequencing results)
- 21:20, 24 January 2022 (diff | hist) . . (-97) . . 20.109(S22):M2D5 (→Part 1: Examine pgRNA sequencing results)
- 21:13, 24 January 2022 (diff | hist) . . (+7,590) . . 20.109(S22):M2D5 (→Protocols)
- 21:12, 24 January 2022 (diff | hist) . . (-7,590) . . 20.109(S22):M2D5 (→Part 2: Examine pgRNA sequencing results)
- 21:09, 24 January 2022 (diff | hist) . . (+10) . . 20.109(S22):M2D4 (→Part 4: Prepare psgRNA clones for sequencing analysis) (current)
- 21:08, 24 January 2022 (diff | hist) . . (-531) . . 20.109(S22):M2D4 (→Part 4: Prepare psgRNA clones for sequencing analysis)
- 20:46, 24 January 2022 (diff | hist) . . (+254) . . 20.109(S22):M2D4 (→Part 2: Mini-prep pgRNA_target clones)
- 20:36, 24 January 2022 (diff | hist) . . (-1,197) . . 20.109(S22):M2D4 (→Part 4: Prepare psgRNA clones for sequencing analysis)
- 20:31, 24 January 2022 (diff | hist) . . (+50) . . 20.109(S22):M2D4 (→Part 4: Prepare psgRNA clones for sequencing analysis)
- 20:27, 24 January 2022 (diff | hist) . . (-42) . . 20.109(S22):M2D4 (→Part 3: Transform CRIPSRi system into MG1655 E. coli cells)
- 20:27, 24 January 2022 (diff | hist) . . (+42) . . 20.109(S22):M2D4 (→Part 3: Transform CRIPSRi system into MG1655 E. coli cells)
- 20:26, 24 January 2022 (diff | hist) . . (+284) . . 20.109(S22):M2D4 (→Part 2: Mini-prep pgRNA_target clones)
- 20:21, 24 January 2022 (diff | hist) . . (0) . . 20.109(S22):M2D2 (→Part 4: Design gRNA for CRISPRi system)
- 20:21, 24 January 2022 (diff | hist) . . (-214) . . 20.109(S22):M2D2 (→Part 4: Design gRNA for CRISPRi system)
- 20:18, 24 January 2022 (diff | hist) . . (-3,613) . . 20.109(S22):M2D5 (→Protocols)
- 20:18, 24 January 2022 (diff | hist) . . (+3,615) . . 20.109(S22):M2D2 (→Protocols)
- 19:58, 24 January 2022 (diff | hist) . . (-113) . . 20.109(S22):M2D4 (→Part 3: Transform CRIPSRi system into MG1655 E. coli cells)
- 19:57, 24 January 2022 (diff | hist) . . (-1,325) . . 20.109(S22):M2D4 (→Protocols)
- 19:50, 24 January 2022 (diff | hist) . . (+29) . . 20.109(S22):M2D2 (→Part 4: Design gRNA for CRISPRi system)
- 19:48, 24 January 2022 (diff | hist) . . (-168) . . 20.109(S22):M2D2 (→Part 4: Design gRNA for CRISPRi system)
- 19:46, 24 January 2022 (diff | hist) . . (-106) . . 20.109(S22):M2D2 (→Part 4: Design gRNA for CRISPRi system)
- 19:42, 24 January 2022 (diff | hist) . . (-422) . . 20.109(S22):M2D5 (→Part 4: Align sgRNA_target sequences to host genome)
- 19:42, 24 January 2022 (diff | hist) . . (+6,146) . . 20.109(S22):M2D5 (→Protocols)
- 19:40, 24 January 2022 (diff | hist) . . (-6,147) . . 20.109(S22):M2D5 (→Part 3: Research CRISPRi system)
- 19:39, 24 January 2022 (diff | hist) . . (+117) . . 20.109(S22):M2D5 (→Reagents list)
- 19:39, 24 January 2022 (diff | hist) . . (+22) . . 20.109(S22):M2D6 (→Reagents list)
- 19:38, 24 January 2022 (diff | hist) . . (+242) . . 20.109(S22):M2D6 (→Part 3: Analyze fermentation product yield data)
- 19:37, 24 January 2022 (diff | hist) . . (-241) . . 20.109(S22):M2D6 (→Measure acetate yield)
- 19:34, 24 January 2022 (diff | hist) . . (-3) . . 20.109(S22):M2D6 (→Measure acetate yield)
- 19:31, 24 January 2022 (diff | hist) . . (+2) . . 20.109(S22):M2D6 (→Measure ethanol yield)
- 19:31, 24 January 2022 (diff | hist) . . (-2) . . 20.109(S22):M2D6 (→Part 2: Measure fermentation product yield)
- 19:30, 24 January 2022 (diff | hist) . . (+1,040) . . 20.109(S22):M2D6 (→Part 1: Measure cell density of cultures)
- 19:29, 24 January 2022 (diff | hist) . . (+8) . . 20.109(S22):M2D6 (→Part 2: Measure fermentation product yield)
- 19:28, 24 January 2022 (diff | hist) . . (-1) . . 20.109(S22):M2D6 (→Part 2: Measure fermentation product yield=)
- 19:28, 24 January 2022 (diff | hist) . . (+4,495) . . 20.109(S22):M2D6 (→Protocols)
- 18:54, 24 January 2022 (diff | hist) . . (+582) . . 20.109(S22):M2D4 (→Reagents list)
- 18:53, 24 January 2022 (diff | hist) . . (+11,366) . . 20.109(S22):M2D4 (→Protocols)
- 18:53, 24 January 2022 (diff | hist) . . (+1,533) . . 20.109(S22):M2D4 (→Protocols)
- 18:52, 24 January 2022 (diff | hist) . . (-1) . . 20.109(S22):M2D4 (→Introduction)
- 18:52, 24 January 2022 (diff | hist) . . (+226) . . 20.109(S22):M2D4 (→Introduction)
- 18:49, 24 January 2022 (diff | hist) . . (-1,639) . . 20.109(S22):M2D5 (→Introduction)
- 18:45, 24 January 2022 (diff | hist) . . (+2,067) . . 20.109(S22):M2D4 (→Protocols)
- 18:43, 24 January 2022 (diff | hist) . . (+2,830) . . 20.109(S22):M2D5 (→Protocols)
- 18:43, 24 January 2022 (diff | hist) . . (-2,835) . . 20.109(S22):M2D4 (→Part 4: Align sgRNA_target sequences to host genome)
- 18:42, 24 January 2022 (diff | hist) . . (+7,593) . . 20.109(S22):M2D5 (→Protocols)
- 18:42, 24 January 2022 (diff | hist) . . (-7,590) . . 20.109(S22):M2D4 (→Part 2: Examine pgRNA sequencing results)
- 18:40, 24 January 2022 (diff | hist) . . (+3,614) . . 20.109(S22):M2D5 (→Protocols)
- 18:37, 24 January 2022 (diff | hist) . . (-3,615) . . 20.109(S22):M2D2 (→Part 3: Research CRISPRi system)
- 18:37, 24 January 2022 (diff | hist) . . (-112) . . 20.109(S22):M2D2 (→Part 4: Design gRNA for CRISPRi system)
- 18:35, 24 January 2022 (diff | hist) . . (-45) . . 20.109(S22):M2D4
- 18:33, 24 January 2022 (diff | hist) . . (+10,426) . . 20.109(S22):M2D4 (→Protocols)
- 17:56, 24 January 2022 (diff | hist) . . (+50) . . 20.109(S22):M2D4 (→Protocols)
- 17:55, 24 January 2022 (diff | hist) . . (+136) . . 20.109(S22):M2D4 (→Protocols)
- 17:50, 24 January 2022 (diff | hist) . . (+8) . . 20.109(S22):M2D3 (→Part 2: Prepare sgRNA_target oligo)
- 17:48, 24 January 2022 (diff | hist) . . (+648) . . 20.109(S22):M2D3 (→Reagents list)
- 17:48, 24 January 2022 (diff | hist) . . (+83) . . 20.109(S22):M2D3 (→Part 3: Insert sgRNA_target sequence into expression plasmid)
- 17:44, 24 January 2022 (diff | hist) . . (+3,659) . . 20.109(S22):M2D3 (→Protocols)
- 17:40, 24 January 2022 (diff | hist) . . (+5) . . 20.109(S22):M2D3 (→Protocols)
- 17:39, 24 January 2022 (diff | hist) . . (+2,047) . . 20.109(S22):M2D3 (→Introduction)
- 17:39, 24 January 2022 (diff | hist) . . (-2,092) . . 20.109(S22):M2D3 (→Synthesize sgRNA_target sequence)
- 17:36, 24 January 2022 (diff | hist) . . (+387) . . 20.109(S22):M2D1 (→Part 3: Ligation of dCas9 insert and expression vector)
- 17:31, 24 January 2022 (diff | hist) . . (+229) . . 20.109(S22):M2D1 (→Part 2: Restriction enzyme digest of expression vector)
- 17:29, 24 January 2022 (diff | hist) . . (+216) . . 20.109(S22):M2D1 (→Part 1: PCR amplification and restriction enzyme digest of dCas9 insert)
- 17:25, 24 January 2022 (diff | hist) . . (-11) . . 20.109(S22):M2D1 (→Part 1: PCR amplification and restriction enzyme digest of dCas9 insert)
- 17:23, 24 January 2022 (diff | hist) . . (+1,372) . . 20.109(S22):M2D1 (→Part 1: PCR amplification and restriction enzyme digest of dCas9 insert)
- 17:00, 24 January 2022 (diff | hist) . . (-5) . . 20.109(S22):M2D1 (→Part 1: PCR amplification and restriction enzyme digest of dCas9 insert)
- 16:59, 24 January 2022 (diff | hist) . . (+12) . . 20.109(S22):M2D1 (→Introduction)
- 15:58, 24 January 2022 (diff | hist) . . (+3) . . 20.109(S22):Module 1 (→Module 1: drug discovery)
- 15:55, 24 January 2022 (diff | hist) . . (+1) . . 20.109(S22):Module 2 (→Module 2: metabolic engineering) (current)
- 15:54, 24 January 2022 (diff | hist) . . (+441) . . 20.109(S22):Module 2 (→Module 2: metabolic engineering)
- 15:47, 24 January 2022 (diff | hist) . . (+11) . . 20.109(S22):Module 2 (→Module 2: metabolic engineering)
- 15:45, 24 January 2022 (diff | hist) . . (+7) . . 20.109(S22):Module 1 (→Module 1: drug discovery)
- 15:43, 24 January 2022 (diff | hist) . . (+152) . . 20.109(S22):Module 2 (→Module 2: metabolic engineering)
- 15:39, 24 January 2022 (diff | hist) . . (+342) . . 20.109(S22):Module 2 (→Module 2: metabolic engineering)
- 15:37, 24 January 2022 (diff | hist) . . (+159) . . 20.109(S22):Module 1 (→Module 1: drug discovery)
- 15:19, 21 January 2022 (diff | hist) . . (+170) . . 20.109(S22):M2D6
- 15:18, 21 January 2022 (diff | hist) . . (+191) . . 20.109(S22):M2D5
- 15:17, 21 January 2022 (diff | hist) . . (+150) . . 20.109(S22):M2D4
- 15:16, 21 January 2022 (diff | hist) . . (+169) . . 20.109(S22):M2D3
- 14:34, 21 January 2022 (diff | hist) . . (0) . . 20.109(S22):Module 3 (→Module 3: Research design)
- 14:34, 21 January 2022 (diff | hist) . . (0) . . 20.109(S22):Module 2 (→Module 2: metabolic engineering)
- 14:34, 21 January 2022 (diff | hist) . . (0) . . 20.109(S22):Module 1 (→Module 1: drug discovery)
- 14:22, 21 January 2022 (diff | hist) . . (+1,604) . . 20.109(S22):M2D3 (→Protocols)
- 14:18, 21 January 2022 (diff | hist) . . (+531) . . 20.109(S22):M2D1 (→Introduction)
- 14:16, 21 January 2022 (diff | hist) . . (+5,416) . . 20.109(S22):M2D3 (→Protocols)
- 14:16, 21 January 2022 (diff | hist) . . (+1,603) . . 20.109(S22):M2D4 (→Protocols)
- 14:14, 21 January 2022 (diff | hist) . . (+171) . . N 20.109(S22):M2D4 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Introduction== ==Protocols== ==Reagents list== ==Navigation links==")
- 14:14, 21 January 2022 (diff | hist) . . (+171) . . N 20.109(S22):M2D3 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Introduction== ==Protocols== ==Reagents list== ==Navigation links==")
- 14:09, 21 January 2022 (diff | hist) . . (+8,882) . . N 20.109(S22):M2D6 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Introduction== ==Protocols== ====Measure cell density of cultures==== Be...")
- 14:07, 21 January 2022 (diff | hist) . . (+2,535) . . 20.109(S22):M2D5
- 14:05, 21 January 2022 (diff | hist) . . (+4,092) . . N 20.109(S22):M2D5 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Introduction== The CRISPRi system involves three genetic components: the p...")
- 13:59, 21 January 2022 (diff | hist) . . (+27) . . 20.109(S22):Module 2 (→Module 2: metabolic engineering)
- 13:58, 21 January 2022 (diff | hist) . . (+78) . . 20.109(S22):Module 2 (→Module 2: metabolic engineering)
- 13:57, 21 January 2022 (diff | hist) . . (0) . . N File:Sp22 M2 overview.png (current)
- 12:50, 21 January 2022 (diff | hist) . . (+80) . . 20.109(S22):Module 1 (→Module 1: drug discovery)
- 12:49, 21 January 2022 (diff | hist) . . (0) . . N File:Sp22 M1 overview.png (current)
- 20:22, 19 January 2022 (diff | hist) . . (+1) . . 20.109(S22):M1D7 (→Complete primary staining steps)
- 20:21, 19 January 2022 (diff | hist) . . (+1) . . 20.109(S22):M1D7 (→Complete secondary staining steps)
- 20:21, 19 January 2022 (diff | hist) . . (+4) . . 20.109(S22):M1D7 (→Reagents list)
- 20:16, 19 January 2022 (diff | hist) . . (+8,655) . . N 20.109(S22):Data Summary (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Overview== The major writing assignment for Module 1 will consist of a tho...")
- 19:43, 19 January 2022 (diff | hist) . . (+13) . . 20.109(S22):M1D8 (→Part 2: Use statistics to interpret results)
- 19:43, 19 January 2022 (diff | hist) . . (-3) . . 20.109(S22):M1D8 (→Part 2: Use statistics to interpret results)
- 19:40, 19 January 2022 (diff | hist) . . (-21) . . 20.109(S22):M1D8 (→Reagents list)
- 19:36, 19 January 2022 (diff | hist) . . (+2,616) . . 20.109(S22):Homework (→Due M2D7)
- 19:34, 19 January 2022 (diff | hist) . . (+2,611) . . 20.109(S22):Homework (→Due M2D8)
- 19:30, 19 January 2022 (diff | hist) . . (+4,016) . . 20.109(S22):Homework (→Due M2D5)
- 19:28, 19 January 2022 (diff | hist) . . (+2,106) . . 20.109(S22):Homework (→Due M2D6)
- 19:20, 19 January 2022 (diff | hist) . . (+6) . . 20.109(S22):FYI (→How to contact the teaching team)
- 19:14, 19 January 2022 (diff | hist) . . (+1,124) . . 20.109(S22):Homework (→Module 3: Research design)
- 19:14, 19 January 2022 (diff | hist) . . (+847) . . 20.109(S22):Homework (→Due M3D2)
- 19:13, 19 January 2022 (diff | hist) . . (+957) . . 20.109(S22):Homework (→Due M3D1)
- 19:11, 19 January 2022 (diff | hist) . . (+1,856) . . 20.109(S22):Homework (→Due M2D4)
- 19:11, 19 January 2022 (diff | hist) . . (+1,891) . . 20.109(S22):Homework (→Due M2D3)
- 19:08, 19 January 2022 (diff | hist) . . (+1,021) . . 20.109(S22):Homework (→Due M2D2)
- 18:31, 19 January 2022 (diff | hist) . . (0) . . 20.109(S22):M1D7 (→Part 2: Perform antibody staining for TDP43-localization experiment)
- 18:25, 19 January 2022 (diff | hist) . . (+550) . . 20.109(S22):M1D7 (→Part 3: something from homework to fill incubation times)
- 18:20, 19 January 2022 (diff | hist) . . (+12) . . 20.109(S22):Homework (→Module 1: Drug discovery)
- 18:18, 19 January 2022 (diff | hist) . . (+173) . . 20.109(S22):Homework (→Due M2D1)
- 18:17, 19 January 2022 (diff | hist) . . (+10) . . 20.109(S22):Homework (→Due M1D8)
- 18:15, 19 January 2022 (diff | hist) . . (-2) . . 20.109(S22):Homework (→Due M1D8)
- 18:15, 19 January 2022 (diff | hist) . . (+1) . . 20.109(S22):Homework (→Due M1D8)
- 18:14, 19 January 2022 (diff | hist) . . (-11) . . 20.109(S22):Homework (→Due M1D8)
- 18:12, 19 January 2022 (diff | hist) . . (+57) . . 20.109(S22):Homework (→Due M1D8)
- 18:10, 19 January 2022 (diff | hist) . . (-11) . . 20.109(S22):Homework (→Due M1D4)
- 18:09, 19 January 2022 (diff | hist) . . (+1,932) . . 20.109(S22):Homework (→Module 1: Drug discovery)
- 18:04, 19 January 2022 (diff | hist) . . (+2,570) . . 20.109(S22):Homework (→Module 1: Drug discovery)
- 17:55, 19 January 2022 (diff | hist) . . (+2,243) . . 20.109(S22):Homework (→Due M1D6)
- 17:51, 19 January 2022 (diff | hist) . . (-1) . . 20.109(S22):Homework (→Due M1D5)
- 17:51, 19 January 2022 (diff | hist) . . (+1,483) . . 20.109(S22):Homework (→Due M1D5)
- 17:44, 19 January 2022 (diff | hist) . . (0) . . 20.109(S22):Homework (→Due M1D6)
- 17:44, 19 January 2022 (diff | hist) . . (+1,783) . . 20.109(S22):Homework (→Due M1D8)
- 17:41, 19 January 2022 (diff | hist) . . (+512) . . 20.109(S22):Homework (→Due M1D5)
- 17:40, 19 January 2022 (diff | hist) . . (+543) . . 20.109(S22):Homework (→Due M1D4)
- 17:40, 19 January 2022 (diff | hist) . . (+544) . . 20.109(S22):Homework (→Due M1D3)
- 17:40, 19 January 2022 (diff | hist) . . (+1,755) . . 20.109(S22):Homework (→Due M1D6)
- 17:37, 19 January 2022 (diff | hist) . . (+2,984) . . 20.109(S22):Homework (→Module 1: Drug discovery)
- 17:12, 19 January 2022 (diff | hist) . . (-74) . . 20.109(S22):M1D2 (→Evaluate raw data for hits)
- 17:11, 19 January 2022 (diff | hist) . . (-1) . . 20.109(S22):M1D2 (→Select small molecule compounds to test in functional assays)
- 17:11, 19 January 2022 (diff | hist) . . (+256) . . 20.109(S22):M1D2 (→Part 2: Complete by-eye analysis of hits identified in SMM using TDP43)
- 17:09, 19 January 2022 (diff | hist) . . (-2) . . 20.109(S22):M1D2 (→Select small molecule compounds to test in functional assays)
- 17:06, 19 January 2022 (diff | hist) . . (+312) . . 20.109(S22):Class data (→small molecule selections)
- 17:06, 19 January 2022 (diff | hist) . . (+312) . . 20.109(S22):Class data
- 17:03, 19 January 2022 (diff | hist) . . (+1) . . 20.109(S22):M1D2 (→Select small molecule compounds to test in functional assays)
- 17:02, 19 January 2022 (diff | hist) . . (-2) . . 20.109(S22):M1D2 (→Select small molecule compounds to test in functional assays)
- 17:02, 19 January 2022 (diff | hist) . . (+814) . . 20.109(S22):M1D2
- 16:48, 19 January 2022 (diff | hist) . . (+188) . . 20.109(S22):M1D2 (→Part 2: Complete by-eye analysis of hits identified in SMM using TDP43)
- 16:48, 19 January 2022 (diff | hist) . . (0) . . N File:07-KI0000907-G08.png (current)
- 16:47, 19 January 2022 (diff | hist) . . (0) . . N File:02-KI0001354-N19.png (current)
- 16:46, 19 January 2022 (diff | hist) . . (0) . . N File:13-KI0001562-P15.png (current)
- 16:45, 19 January 2022 (diff | hist) . . (0) . . N File:05-KI0001106-N03.png (current)
- 16:44, 19 January 2022 (diff | hist) . . (-6) . . 20.109(S22):M1D2 (→Part 2: Complete by-eye analysis of hits identified in SMM using TDP43)
- 16:43, 19 January 2022 (diff | hist) . . (+13) . . 20.109(S22):M1D2 (→Part 2: Complete by-eye analysis of hits identified in SMM using TDP43)
- 16:42, 19 January 2022 (diff | hist) . . (+15) . . 20.109(S22):M1D2 (→Part 2: Complete by-eye analysis of hits identified in SMM using TDP43)
- 16:42, 19 January 2022 (diff | hist) . . (-18) . . 20.109(S22):M1D2 (→Part 2: Complete by-eye analysis of hits identified in SMM using TDP43)
- 16:41, 19 January 2022 (diff | hist) . . (+565) . . 20.109(S22):M1D2 (→Part 2: Complete by-eye analysis of hits identified in SMM using TDP43)
- 16:40, 19 January 2022 (diff | hist) . . (0) . . N File:Sp22 M1D2 01-KI000454-E08.png (current)
- 16:26, 19 January 2022 (diff | hist) . . (+4) . . 20.109(S22):M1D2 (→Evaluate raw data for hits)
- 16:25, 19 January 2022 (diff | hist) . . (+150) . . 20.109(S22):M1D2 (→Part 2: Visually evaluate chemical structures of putative binders)
- 16:12, 19 January 2022 (diff | hist) . . (+52) . . 20.109(S22):M1D7 (→Part 1: Treat cells for TDP43-localization experiment)
- 16:11, 19 January 2022 (diff | hist) . . (0) . . N File:Sp22 M1D7 localization plate map.png (current)
- 15:54, 19 January 2022 (diff | hist) . . (-2) . . 20.109(S22):M1D7 (→Part 1: Treat cells for TDP43-localization experiment)
- 15:54, 19 January 2022 (diff | hist) . . (+1,535) . . 20.109(S22):M1D7 (→Protocols)
- 15:40, 19 January 2022 (diff | hist) . . (+464) . . 20.109(S22):M1D2 (→Part 2: Visually evaluate chemical structures of putative binders)
- 13:01, 19 January 2022 (diff | hist) . . (+5) . . 20.109(S22):FYI (→How to contact the teaching team)
- 22:32, 18 January 2022 (diff | hist) . . (+44) . . 20.109(S22):M1D8 (→Part 2: Use statistics to interpret results)
- 22:28, 18 January 2022 (diff | hist) . . (+463) . . 20.109(S22):M1D7 (→Part 1: Perform antibody staining for TDP43-localization experiment)
- 22:14, 18 January 2022 (diff | hist) . . (+59) . . 20.109(S22):M1D7 (→Protocols)
- 22:12, 18 January 2022 (diff | hist) . . (+52) . . 20.109(S22):M1D7 (→Protocols)
- 22:09, 18 January 2022 (diff | hist) . . (+349) . . 20.109(S22):M1D7 (→Reagents list)
- 22:08, 18 January 2022 (diff | hist) . . (-8) . . 20.109(S22):M1D7 (→Protocols)
- 22:06, 18 January 2022 (diff | hist) . . (-319) . . 20.109(S22):M1D7 (→Part 2: Perform antibody staining for TDP43-localization experiment)
- 21:59, 18 January 2022 (diff | hist) . . (+12) . . 20.109(S22):M1D7 (→Part 2: Perform antibody staining for γH2AX assay)
- 21:48, 18 January 2022 (diff | hist) . . (-268) . . 20.109(S22):M1D7 (→Introduction)
- 21:46, 18 January 2022 (diff | hist) . . (+1) . . 20.109(S22):M1D8 (→Part 1: Image and analyze TDP43 localization experiment results)
- 21:45, 18 January 2022 (diff | hist) . . (+2,117) . . 20.109(S22):M1D8 (→Part 1: Image and analyze TDP43 localization experiment results)
- 21:41, 18 January 2022 (diff | hist) . . (+5,332) . . 20.109(S22):M1D7 (→Protocols)
- 21:21, 18 January 2022 (diff | hist) . . (0) . . 20.109(S22):Research proposal presentation (→Logistics) (current)
- 21:21, 18 January 2022 (diff | hist) . . (+27,441) . . N 20.109(S22):Research article (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Overview== The culminating assignment for Module 2 will is the Research ar...") (current)
- 21:15, 18 January 2022 (diff | hist) . . (+11,074) . . N 20.109(S22):Research proposal presentation (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Overview== Writing a research proposal requires that you identify an inte...")
- 21:09, 18 January 2022 (diff | hist) . . (-4) . . 20.109(S22):Spring 2022 schedule
- 20:35, 18 January 2022 (diff | hist) . . (+4,625) . . N 20.109(S22):Research talk (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Overview== In addition to the Data Summary, you will complete a short Rese...") (current)
- 20:04, 18 January 2022 (diff | hist) . . (0) . . 20.109(S22):Module 3 (→Module 3: Research design)
- 20:04, 18 January 2022 (diff | hist) . . (-1) . . 20.109(S22):Module 3 (→Major assignment)
- 20:04, 18 January 2022 (diff | hist) . . (+80) . . 20.109(S22):Module 3
- 20:03, 18 January 2022 (diff | hist) . . (-487) . . 20.109(S22):Module 3 (→Module 3: Research design)
- 20:02, 18 January 2022 (diff | hist) . . (+3,031) . . N 20.109(S22):Module 3 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Module 3: Research design== At the core of scientific discovery is buildi...")
- 19:59, 18 January 2022 (diff | hist) . . (0) . . 20.109(S22):Assignments (→Major assignments)
- 19:58, 18 January 2022 (diff | hist) . . (-31) . . 20.109(S22):Module 1 (→Major assignments)
- 19:57, 18 January 2022 (diff | hist) . . (+1,499) . . N 20.109(S22):Module 2 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Module 2: metabolic engineering== <font color = #0d368e>'''Research goa...")
- 19:51, 18 January 2022 (diff | hist) . . (0) . . 20.109(S22):Module 1 (→Notes for Instructors)
- 19:51, 18 January 2022 (diff | hist) . . (+3,069) . . N 20.109(S22):Module 1 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Module 1: drug discovery== Small molecules, or ligands, are important rese...")
- 19:45, 18 January 2022 (diff | hist) . . (0) . . 20.109(S22):M1D5 (→Protocols)
- 19:43, 18 January 2022 (diff | hist) . . (+7) . . 20.109(S22):FYI (→How to contact the teaching team)
- 19:36, 18 January 2022 (diff | hist) . . (-497) . . 20.109(S22):M1D2 (→Part 2: Visually evaluate chemical structures of putative binders)
- 19:32, 18 January 2022 (diff | hist) . . (+924) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 19:16, 18 January 2022 (diff | hist) . . (+897) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 19:09, 18 January 2022 (diff | hist) . . (0) . . N File:Sp22 M1D2 by eye analysis.png (current)
- 19:02, 18 January 2022 (diff | hist) . . (+936) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 19:00, 18 January 2022 (diff | hist) . . (0) . . N File:Sp22 M1D2 binned z score graph.png (current)
- 18:53, 18 January 2022 (diff | hist) . . (+957) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 18:42, 18 January 2022 (diff | hist) . . (0) . . N File:Sp22 M1D2 average z score graph.png (current)
- 18:39, 18 January 2022 (diff | hist) . . (+633) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 18:27, 18 January 2022 (diff | hist) . . (0) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 18:26, 18 January 2022 (diff | hist) . . (+344) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 18:15, 18 January 2022 (diff | hist) . . (+61) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 18:12, 18 January 2022 (diff | hist) . . (+1) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 18:12, 18 January 2022 (diff | hist) . . (+7) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 18:11, 18 January 2022 (diff | hist) . . (+117) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 17:54, 18 January 2022 (diff | hist) . . (+21) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 17:50, 18 January 2022 (diff | hist) . . (+362) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 15:53, 18 January 2022 (diff | hist) . . (+239) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 15:48, 18 January 2022 (diff | hist) . . (0) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 15:48, 18 January 2022 (diff | hist) . . (+59) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 15:47, 18 January 2022 (diff | hist) . . (0) . . N File:Sp22 M1D2 P1 quantify 635nm.png (current)
- 15:46, 18 January 2022 (diff | hist) . . (0) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 15:45, 18 January 2022 (diff | hist) . . (+7) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 15:44, 18 January 2022 (diff | hist) . . (+766) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 15:34, 18 January 2022 (diff | hist) . . (0) . . N File:Sp22 M1D2 P1 align spots.png (current)
- 15:11, 18 January 2022 (diff | hist) . . (+218) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 15:08, 18 January 2022 (diff | hist) . . (-1) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 15:08, 18 January 2022 (diff | hist) . . (+197) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 15:04, 18 January 2022 (diff | hist) . . (0) . . N File:Sp22 M1D2 slide, block.png (current)
- 14:57, 18 January 2022 (diff | hist) . . (+1,178) . . 20.109(S22):M1D2 (→Part 1: Analyze SMM results)
- 14:09, 18 January 2022 (diff | hist) . . (+6) . . 20.109(S22):M1D2 (→Introduction)
- 21:24, 14 January 2022 (diff | hist) . . (+25) . . 20.109(S22):Spring 2022 schedule
- 18:51, 14 January 2022 (diff | hist) . . (-12) . . 20.109(S22):M3D3 (→Navigation links) (current)
- 18:50, 14 January 2022 (diff | hist) . . (+73) . . 20.109(S22):M3D2 (→Navigation links) (current)
- 18:50, 14 January 2022 (diff | hist) . . (+79) . . 20.109(S22):M3D1 (→Navigation links) (current)
- 18:49, 14 January 2022 (diff | hist) . . (+79) . . 20.109(S22):M2D8 (→Navigation links)
- 18:47, 14 January 2022 (diff | hist) . . (-1) . . 20.109(S22):M2D7 (→Navigation links)
- 18:47, 14 January 2022 (diff | hist) . . (+93) . . 20.109(S22):M2D7 (→Navigation links)
- 18:39, 14 January 2022 (diff | hist) . . (-10) . . 20.109(S22):M2D1 (→Introduction)
- 18:34, 14 January 2022 (diff | hist) . . (-267) . . 20.109(S22):M1D4 (→Part 3: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 18:33, 14 January 2022 (diff | hist) . . (-256) . . 20.109(S22):M1D4 (→Part 3: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 18:27, 14 January 2022 (diff | hist) . . (+67) . . 20.109(S22):Laboratory tour (→Station 9: Introduction to tissue culture facility)
- 18:26, 14 January 2022 (diff | hist) . . (+111) . . 20.109(S22):Laboratory tour (→Station 4: Introduction to using a gel documentation system)
- 18:23, 14 January 2022 (diff | hist) . . (0) . . N File:109 Orientation gel doc and gel.png (current)
- 18:19, 14 January 2022 (diff | hist) . . (+1) . . 20.109(S22):Laboratory tour (→Part 1: Measuring absorbance)
- 18:18, 14 January 2022 (diff | hist) . . (0) . . 20.109(S22):Laboratory tour (→Part 1: Measuring absorbance)
- 18:18, 14 January 2022 (diff | hist) . . (0) . . 20.109(S22):Laboratory tour (→Part 1: Measuring absorbance)
- 18:18, 14 January 2022 (diff | hist) . . (0) . . 20.109(S22):Laboratory tour (→Part 1: Measuring absorbance)
- 18:17, 14 January 2022 (diff | hist) . . (-67) . . 20.109(S22):Laboratory tour (→Station 3: Introduction to using a spectrophotometer)
- 18:14, 14 January 2022 (diff | hist) . . (-1) . . 20.109(S22):Laboratory tour (→Part 1: Measuring solids)
- 18:14, 14 January 2022 (diff | hist) . . (0) . . 20.109(S22):Laboratory tour (→Part 1: Measuring solids)
- 18:13, 14 January 2022 (diff | hist) . . (+36) . . 20.109(S22):Laboratory tour (→Part 1: Measuring solids)
- 18:12, 14 January 2022 (diff | hist) . . (+29) . . 20.109(S22):Laboratory tour (→Part 1: Measuring absorbance)
- 18:11, 14 January 2022 (diff | hist) . . (0) . . N File:109 Orientation spec and scan.png (current)
- 18:05, 14 January 2022 (diff | hist) . . (+16) . . 20.109(S22):Homework (→Module 2: Metabolic engineering)
- 18:05, 14 January 2022 (diff | hist) . . (+3) . . 20.109(S22):Homework (→Homework Submission Guidelines)
- 18:04, 14 January 2022 (diff | hist) . . (+3) . . 20.109(S22):Assignments (→Homework Submission Guidelines)
- 18:02, 14 January 2022 (diff | hist) . . (+1,400) . . 20.109(S22):Class data
- 17:47, 14 January 2022 (diff | hist) . . (+6) . . 20.109(S22):M1D6 (→Part 2: Seed cells for aggregation assay)
- 17:46, 14 January 2022 (diff | hist) . . (0) . . 20.109(S22):M1D6 (→Introduction)
- 17:45, 14 January 2022 (diff | hist) . . (0) . . 20.109(S22):M1D6 (→Introduction)
- 17:45, 14 January 2022 (diff | hist) . . (0) . . 20.109(S22):M1D6 (→Introduction)
- 21:10, 13 January 2022 (diff | hist) . . (-1) . . 20.109(S22):Spring 2022 schedule
- 21:09, 13 January 2022 (diff | hist) . . (+48) . . 20.109(S22):Spring 2022 schedule
- 16:39, 13 January 2022 (diff | hist) . . (-3) . . 20.109(S22):Spring 2022 schedule
- 16:37, 13 January 2022 (diff | hist) . . (+71) . . 20.109(S22):Spring 2022 schedule
- 16:05, 13 January 2022 (diff | hist) . . (+23) . . 20.109(S22):M2D2 (→Part 4: Design gRNA for CRISPRi system)
- 16:05, 13 January 2022 (diff | hist) . . (+202) . . 20.109(S22):M2D2 (→Reagents list)
- 16:04, 13 January 2022 (diff | hist) . . (+1,456) . . 20.109(S22):M2D2 (→Part 4: Design gRNA for CRISPRi system)
- 15:57, 13 January 2022 (diff | hist) . . (+88) . . 20.109(S22):M2D2 (→Part 2: Review E. coli anaerobic fermentative metabolism)
- 15:55, 13 January 2022 (diff | hist) . . (+13,896) . . 20.109(S22):M2D2
- 15:49, 13 January 2022 (diff | hist) . . (+3,598) . . N 20.109(S22):M2D2 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Introduction== In this module you will engineer the metabolism of ''E. col...")
- 15:37, 13 January 2022 (diff | hist) . . (-5) . . 20.109(S22):M2D1 (→Part 4: Confirmation digest of pdCas9 expression plasmid)
- 15:37, 13 January 2022 (diff | hist) . . (+496) . . 20.109(S22):M2D1 (→Part 4: Confirmation digest of pdCas9 expression plasmid)
- 15:33, 13 January 2022 (diff | hist) . . (+29) . . 20.109(S22):M2D1 (→Part 4: Confirmation digest)
- 15:31, 13 January 2022 (diff | hist) . . (-68) . . 20.109(S22):M2D1 (→Part 4: Confirmation digest)
- 15:30, 13 January 2022 (diff | hist) . . (-1) . . 20.109(S22):M2D1 (→Navigation links)
- 15:30, 13 January 2022 (diff | hist) . . (+11) . . 20.109(S22):M2D1 (→Part 2: Restriction enzyme digest of vector)
- 15:29, 13 January 2022 (diff | hist) . . (+17,718) . . 20.109(S22):M2D1
- 15:25, 13 January 2022 (diff | hist) . . (+6,131) . . N 20.109(S22):M2D1 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Introduction== Though the theme of Module 2 is metabolic engineering, tod...")
- 15:17, 13 January 2022 (diff | hist) . . (+5,450) . . 20.109(S22):M1D8
- 14:59, 13 January 2022 (diff | hist) . . (0) . . 20.109(S22):M1D2 (→Introduction)
- 14:59, 13 January 2022 (diff | hist) . . (+11) . . 20.109(S22):M1D2 (→Introduction)
- 14:54, 13 January 2022 (diff | hist) . . (+1,109) . . 20.109(S22):M1D2 (→Introduction)
- 14:45, 13 January 2022 (diff | hist) . . (+106) . . 20.109(S22):M1D2
- 14:39, 13 January 2022 (diff | hist) . . (+1) . . 20.109(S22):M1D2 (→Part 3: Review journal article)
- 14:39, 13 January 2022 (diff | hist) . . (+298) . . 20.109(S22):M1D2 (→Part 3: Review journal article)
- 14:32, 13 January 2022 (diff | hist) . . (-265) . . 20.109(S22):M1D3 (→Part 2: Purify TDP43-RRM12 protein)
- 14:31, 13 January 2022 (diff | hist) . . (+37) . . 20.109(S22):M1D3 (→Part 1: Induce expression of TDP43-RRM12)
- 14:28, 13 January 2022 (diff | hist) . . (-241) . . 20.109(S22):M1D4 (→Part 4: Measure protein concentration)
- 14:27, 13 January 2022 (diff | hist) . . (+3) . . 20.109(S22):M1D4 (→Part 3: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 14:27, 13 January 2022 (diff | hist) . . (-575) . . 20.109(S22):M1D4 (→Part 3: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 14:23, 13 January 2022 (diff | hist) . . (0) . . 20.109(S22):M1D4 (→Part 3: Measure protein concentration)
- 14:22, 13 January 2022 (diff | hist) . . (+184) . . 20.109(S22):M1D4 (→Protocols)
- 14:20, 13 January 2022 (diff | hist) . . (+184) . . 20.109(S22):M1D5 (→Protocols)
- 14:19, 13 January 2022 (diff | hist) . . (+54) . . 20.109(S22):M1D5 (→Part 2: Draft Data summary slide for γH2AX results)
- 14:18, 13 January 2022 (diff | hist) . . (+778) . . 20.109(S22):M1D5 (→Protocols)
- 14:14, 13 January 2022 (diff | hist) . . (+299) . . 20.109(S22):M1D7
- 14:12, 13 January 2022 (diff | hist) . . (+111) . . 20.109(S22):M1D6 (→Navigation links)
- 14:11, 13 January 2022 (diff | hist) . . (+354) . . N 20.109(S22):M1D8 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Introduction== ==Protocols== ==Reagents list== ==Navigation links== N...")
- 14:10, 13 January 2022 (diff | hist) . . (+410) . . N 20.109(S22):M1D5 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Introduction== ==Protocols== ==Reagents list== ==Navigation links== N...")
- 14:09, 13 January 2022 (diff | hist) . . (+96) . . 20.109(S22):M1D4 (→Navigation links)
- 14:08, 13 January 2022 (diff | hist) . . (+80) . . 20.109(S22):M1D3 (→Navigation links)
- 14:08, 13 January 2022 (diff | hist) . . (+58) . . 20.109(S22):M1D2 (→Navigation links)
- 14:07, 13 January 2022 (diff | hist) . . (+35) . . 20.109(S22):M1D1 (→Navigation links)
- 14:06, 13 January 2022 (diff | hist) . . (-7) . . 20.109(S22):Spring 2022 schedule
- 13:56, 13 January 2022 (diff | hist) . . (+103) . . 20.109(S22):M2D7
- 13:55, 13 January 2022 (diff | hist) . . (-6,988) . . 20.109(S22):M2D7 (→Protocols)
- 13:55, 13 January 2022 (diff | hist) . . (-3,644) . . 20.109(S22):M2D7 (→Introduction)
- 13:55, 13 January 2022 (diff | hist) . . (+10,645) . . 20.109(S22):M2D7
- 13:48, 13 January 2022 (diff | hist) . . (+22) . . 20.109(S22):M2D7
- 13:39, 13 January 2022 (diff | hist) . . (-10) . . 20.109(S22):M3D1 (→Exercises)
- 13:36, 13 January 2022 (diff | hist) . . (+103) . . 20.109(S22):M3D3
- 13:35, 13 January 2022 (diff | hist) . . (+5,246) . . N 20.109(S22):M3D3 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Exercises== ===Part 1: Discuss Research proposal with peers=== To help y...")
- 13:35, 13 January 2022 (diff | hist) . . (0) . . 20.109(S22):Spring 2022 schedule
- 13:35, 13 January 2022 (diff | hist) . . (-5,246) . . 20.109(S22):M3D4 (Blanked the page) (current)
- 13:33, 13 January 2022 (diff | hist) . . (+103) . . 20.109(S22):M3D2
- 13:33, 13 January 2022 (diff | hist) . . (+103) . . 20.109(S22):M3D1
- 13:32, 13 January 2022 (diff | hist) . . (+104) . . 20.109(S22):M2D8
- 13:30, 13 January 2022 (diff | hist) . . (+1,874) . . 20.109(S22):M3D4 (→Exercises)
- 13:29, 13 January 2022 (diff | hist) . . (-879) . . 20.109(S22):M3D2 (→Exercises)
- 13:28, 13 January 2022 (diff | hist) . . (+3,372) . . N 20.109(S22):M3D4 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Exercises== ===Part 1: Discuss Research proposal with peers=== To help y...")
- 13:27, 13 January 2022 (diff | hist) . . (+3,407) . . N 20.109(S22):M3D2 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Exercises== ===Part 1: Draft aims for Research proposal presentation=== ...")
- 13:25, 13 January 2022 (diff | hist) . . (0) . . 20.109(S22):M2D8 (→Part 1: Organize figures and outline results text for your Research article)
- 13:24, 13 January 2022 (diff | hist) . . (-3) . . 20.109(S22):M2D8 (→Part 2: Write your Research article)
- 13:24, 13 January 2022 (diff | hist) . . (+215) . . 20.109(S22):M2D8 (→Exercises)
- 13:21, 13 January 2022 (diff | hist) . . (+45) . . 20.109(S22):M2D8
- 13:20, 13 January 2022 (diff | hist) . . (+2,110) . . N 20.109(S22):M3D1 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Exercises== ===Part 1: Discuss potential topics for your Research proposa...")
- 13:18, 13 January 2022 (diff | hist) . . (-2) . . 20.109(S22):Spring 2022 schedule
- 13:15, 13 January 2022 (diff | hist) . . (+4,538) . . N 20.109(S22):M1D7 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Introduction== In the TDP43-localization experiment, you will use commerci...")
- 13:08, 13 January 2022 (diff | hist) . . (+7,975) . . N 20.109(S22):M1D2 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Introduction== ==Protocols== ===Part 1: Analyze SMM results=== Image:S...")
- 13:03, 13 January 2022 (diff | hist) . . (+399) . . 20.109(S22):M1D1
- 13:01, 13 January 2022 (diff | hist) . . (-12) . . 20.109(S22):M1D1 (→Protocols)
- 13:00, 13 January 2022 (diff | hist) . . (-393) . . 20.109(S22):M1D1
- 12:51, 13 January 2022 (diff | hist) . . (+548) . . 20.109(S22):M1D6
- 12:50, 13 January 2022 (diff | hist) . . (+15) . . 20.109(S22):M1D6 (→Introduction)
- 12:49, 13 January 2022 (diff | hist) . . (+3,756) . . 20.109(S22):M1D6
- 12:42, 13 January 2022 (diff | hist) . . (+6,399) . . N 20.109(S22):M1D6 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ===Part 1: Learn cell culture best practices=== One major objective for th...")
- 12:38, 13 January 2022 (diff | hist) . . (+305) . . 20.109(S22):M1D1 (→Protocols)
- 12:37, 13 January 2022 (diff | hist) . . (+11,325) . . N 20.109(S22):M1D1 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Introduction== A small molecule microarray (SMM) requires the covalent att...")
- 12:32, 13 January 2022 (diff | hist) . . (+16,158) . . N 20.109(S22):M1D4 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Introduction== Two metrics will be used to evaluate the success of the pr...")
- 12:30, 13 January 2022 (diff | hist) . . (+12,292) . . N 20.109(S22):M1D3 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Introduction== The TDP43 protein is encoded by the TARDBP gene. The entire...")
- 18:12, 12 January 2022 (diff | hist) . . (-1) . . 20.109(S22):Spring 2022 schedule
- 17:58, 12 January 2022 (diff | hist) . . (+19) . . 20.109(S22):Spring 2022 schedule
- 17:56, 12 January 2022 (diff | hist) . . (0) . . 20.109(S22):Spring 2022 schedule
- 17:55, 12 January 2022 (diff | hist) . . (+209) . . 20.109(S22):Spring 2022 schedule
- 17:49, 12 January 2022 (diff | hist) . . (+381) . . 20.109(S22):Spring 2022 schedule
- 17:23, 12 January 2022 (diff | hist) . . (-1) . . Template:20.109(S22) (current)
- 17:22, 12 January 2022 (diff | hist) . . (-16) . . 20.109(S22):Homework
- 17:18, 12 January 2022 (diff | hist) . . (0) . . 20.109(S22):Spring 2022 schedule
- 17:11, 12 January 2022 (diff | hist) . . (+203) . . 20.109(S22):Spring 2022 schedule
- 16:57, 12 January 2022 (diff | hist) . . (-132) . . 20.109(S22):Spring 2022 schedule
- 16:14, 12 January 2022 (diff | hist) . . (+133) . . 20.109(S22):Spring 2022 schedule
- 16:05, 12 January 2022 (diff | hist) . . (+301) . . 20.109(S22):Spring 2022 schedule
- 16:03, 12 January 2022 (diff | hist) . . (+5) . . 20.109(S22):FYI (→How to contact the teaching team)
- 16:02, 12 January 2022 (diff | hist) . . (+42) . . 20.109(S22):Spring 2022 schedule
- 16:01, 12 January 2022 (diff | hist) . . (+1,757) . . N 20.109(S22):M2D8 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ===Organize figures and outline results text for your Research article=== T...")
- 15:59, 12 January 2022 (diff | hist) . . (0) . . 20.109(S22):Spring 2022 schedule
- 15:58, 12 January 2022 (diff | hist) . . (+40) . . 20.109(S22):Spring 2022 schedule
- 15:57, 12 January 2022 (diff | hist) . . (+10,729) . . N 20.109(S22):M2D7 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Introduction== Image:Fa20 M3D4 RNAP and sigma factor v2.png|right|500px...")
- 15:53, 12 January 2022 (diff | hist) . . (+950) . . 20.109(S22):Homework
- 15:48, 12 January 2022 (diff | hist) . . (+5) . . 20.109(S22):Communication (→Workshop Slides and Recordings)
- 15:47, 12 January 2022 (diff | hist) . . (+2,200) . . N 20.109(S22):Communication (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==BE Communication Lab== Throughout the semester you will participate in wo...")
- 15:46, 12 January 2022 (diff | hist) . . (+95) . . N 20.109(S22):Class data (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}}")
- 15:46, 12 January 2022 (diff | hist) . . (+95) . . N 20.109(S22):Homework (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}}")
- 15:45, 12 January 2022 (diff | hist) . . (+13) . . 20.109(S22):Assignments (→Contribution to Class blog)
- 15:45, 12 January 2022 (diff | hist) . . (-48) . . 20.109(S22):Assignments (→Contribution to Class blog)
- 15:43, 12 January 2022 (diff | hist) . . (+13,768) . . N 20.109(S22):Assignments (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==Overview== You will complete three experimental modules over the course of...")
- 15:39, 12 January 2022 (diff | hist) . . (+1,123) . . N 20.109(S22):FYI (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S22)}} ==How to contact the teaching team== '''Instructors:''' * [http://be.mit.edu...")
- 15:33, 12 January 2022 (diff | hist) . . (0) . . 20.109(S22):Laboratory tour (→Station 8: Introduction to course wiki)
- 15:33, 12 January 2022 (diff | hist) . . (0) . . N File:Sp22 toolbar image.png (current)
- 15:31, 12 January 2022 (diff | hist) . . (0) . . 20.109(S22):Laboratory tour (→Laboratory notebook template)
- 15:30, 12 January 2022 (diff | hist) . . (0) . . 20.109(S22):Laboratory tour (→Laboratory notebook template)
- 15:30, 12 January 2022 (diff | hist) . . (0) . . 20.109(S22):Laboratory tour (→Orientation questionnaire)
- 15:29, 12 January 2022 (diff | hist) . . (-1) . . 20.109(S22):Laboratory tour
- 15:29, 12 January 2022 (diff | hist) . . (+96) . . 20.109(S22):Laboratory tour
- 15:28, 12 January 2022 (diff | hist) . . (+34,976) . . N 20.109(S22):Laboratory tour (Created page with " =Introduction= The main goals for today are to familiarize yourself with the space and equipment in the teaching laboratory. In this, you will work in partners to complete ...")
- 21:07, 5 January 2022 (diff | hist) . . (-1) . . 20.109(S22):Spring 2022 schedule
- 17:35, 5 January 2022 (diff | hist) . . (+94) . . 20.109(S22):Spring 2022 schedule
- 17:32, 5 January 2022 (diff | hist) . . (+57) . . 20.109(S22):Spring 2022 schedule
- 17:30, 5 January 2022 (diff | hist) . . (+1) . . 20.109(S22):Spring 2022 schedule
- 17:30, 5 January 2022 (diff | hist) . . (-113) . . 20.109(S22):Spring 2022 schedule
- 17:22, 5 January 2022 (diff | hist) . . (-2,590) . . 20.109(S22):Spring 2022 schedule
- 16:57, 5 January 2022 (diff | hist) . . (-40) . . 20.109(S22):Spring 2022 schedule
- 16:56, 5 January 2022 (diff | hist) . . (-2,175) . . 20.109(S22):Spring 2022 schedule
- 16:38, 5 January 2022 (diff | hist) . . (0) . . 20.109(S22):Spring 2022 schedule
- 16:37, 5 January 2022 (diff | hist) . . (0) . . 20.109(S22):Spring 2022 schedule
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- 16:35, 5 January 2022 (diff | hist) . . (0) . . 20.109(S22):Spring 2022 schedule
- 16:34, 5 January 2022 (diff | hist) . . (+571) . . 20.109(S22):Spring 2022 schedule
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