Difference between revisions of "Assignment 5 Overview"
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#* Plot two or more example bead trajectories for each of the samples. (Hint: If you subtract the initial position from each trajectory, then you can plot multiple trajectories on a single set of axes.) | #* Plot two or more example bead trajectories for each of the samples. (Hint: If you subtract the initial position from each trajectory, then you can plot multiple trajectories on a single set of axes.) | ||
# Analysis and Results | # Analysis and Results | ||
− | #* Plot the | + | #* Plot the MSD vs time (from the difference trajectories) for untreated and Cyto D treated cells on a single set of log-log axes. (It is fine to either plot the average of the MSDs for each condition, or to plot the individual MSDs from each pair of beads in each movie. Do not include MSDs of particles not endocytosed by the cells.) |
# Discussion | # Discussion | ||
#* What kind of motion do you see described by your MSD vs τ results? | #* What kind of motion do you see described by your MSD vs τ results? |
Revision as of 15:22, 14 March 2019
Assignment details
This assignment has 2 parts:
- Part 1: Implementing difference tracking and measuring the stability of your microscope;
- Part 2: Live cell particle tracking of endocytosed beads.
Submit your work in on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment5.pdf.
Code examples and simulations
- Converting Gaussian fit to Rayleigh resolution
- Matlab: Scalebars
- Calculating MSD and Diffusion Coefficients
- Overview
- Part 1: MSD difference tracking and microscope stability
- Part 2: Live cell particle tracking of endocytosed beads
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