Difference between revisions of "20.109(S23):Spring 2023 schedule"
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Noreen Lyell (Talk | contribs) |
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| Line 29: | Line 29: | ||
| R/F Feb 9/10 | | R/F Feb 9/10 | ||
| [ AK] <br> | | [ AK] <br> | ||
| − | | [[20.109(S23):M1D1 | | + | | [[20.109(S23):M1D1 | Complete in-silico cloning of protein expression plasmid]]<br> |
| <font color= #0aa24d >'''Orientation quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D1|Homework due]] | | <font color= #0aa24d >'''Orientation quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D1|Homework due]] | ||
|-- | |-- | ||
| Line 35: | Line 35: | ||
| T/W Feb 14/15 | | T/W Feb 14/15 | ||
| [ AK] <br> | | [ AK] <br> | ||
| − | | [[20.109(S23):M1D2 | | + | | [[20.109(S23):M1D2 |Perform protein purification protocol]] <br> |
| [[20.109(S23):Homework#Due_M1D2|Homework due]] | | [[20.109(S23):Homework#Due_M1D2|Homework due]] | ||
|-- | |-- | ||
| Line 41: | Line 41: | ||
| R/F Feb 16/17 | | R/F Feb 16/17 | ||
| [ AK] <br> | | [ AK] <br> | ||
| − | | [[20.109(S23):M1D3 | | + | | [[20.109(S23):M1D3 |Assess purity and concentration of purified protein]] <br> |
| [[20.109(S23):Homework#Due_M1D3|Homework due]] | | [[20.109(S23):Homework#Due_M1D3|Homework due]] | ||
|-- | |-- | ||
| Line 53: | Line 53: | ||
| R/F Feb 23/24 | | R/F Feb 23/24 | ||
| [ AK] <br> | | [ AK] <br> | ||
| − | | [[20.109(S23):M1D4 | | + | | [[20.109(S23):M1D4 |Confirm purified protein using Western blot]] <br> |
| <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D4|Homework due]] | | <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D4|Homework due]] | ||
|-- | |-- | ||
| Line 59: | Line 59: | ||
| T/W Feb/Mar 28/1 | | T/W Feb/Mar 28/1 | ||
| [ AK] <br> | | [ AK] <br> | ||
| − | | [[20.109(S23):M1D5 | | + | | [[20.109(S23):M1D5 |Image Western blot of purified protein]] <br> |
| [[20.109(S23):Homework#Due_M1D5|Homework due]] <br> [[20.109(S23):Research talk| <font color = #b3251c>'''Research talk due'''</font color>]] XX | | [[20.109(S23):Homework#Due_M1D5|Homework due]] <br> [[20.109(S23):Research talk| <font color = #b3251c>'''Research talk due'''</font color>]] XX | ||
|-- | |-- | ||
| Line 65: | Line 65: | ||
| R/F Mar 2/3 | | R/F Mar 2/3 | ||
| [ AK] <br> | | [ AK] <br> | ||
| − | | [[20.109(S23):M1D6 | | + | | [[20.109(S23):M1D6 |Prepare and scan small molecule microarray (SMM) slides]] <br> |
| [[20.109(S23):Homework#Due_M1D6|Homework due]] | | [[20.109(S23):Homework#Due_M1D6|Homework due]] | ||
|-- | |-- | ||
| Line 71: | Line 71: | ||
| T/W Mar 7/8 | | T/W Mar 7/8 | ||
| [ AK] <br> | | [ AK] <br> | ||
| − | | [[20.109(S23):M1D7 | | + | | [[20.109(S23):M1D7 |Analyze SMM data to identify putative small molecule binders]] <br> |
| − | | | + | | [[20.109(S23):Homework#Due_M1D7|Homework due]] |
|-- | |-- | ||
| − | | | + | | M1D8 |
| R/F Mar 9/10 | | R/F Mar 9/10 | ||
| + | | [ AK] <br> | ||
| + | | [[20.109(S23):M1D8 |Organize Data summary figures and results]] <br> | ||
| + | | <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D8|Homework due]] | ||
| + | |-- | ||
| + | | M2D1 | ||
| + | | T/W Mar 14/15 | ||
| [ BCM] <br> | | [ BCM] <br> | ||
| [[20.109(S23):M2D1 |Determine mutagenesis strategy]] <br> | | [[20.109(S23):M2D1 |Determine mutagenesis strategy]] <br> | ||
| Line 81: | Line 87: | ||
|-- | |-- | ||
| M2D2 | | M2D2 | ||
| − | | | + | | R/F Mar 16/17 |
| [ RCM] <br> | | [ RCM] <br> | ||
| [[20.109(S23):M2D2 |Perform site-directed mutagenesis]] <br> | | [[20.109(S23):M2D2 |Perform site-directed mutagenesis]] <br> | ||
| Line 87: | Line 93: | ||
|-- | |-- | ||
| M2D3 | | M2D3 | ||
| − | | | + | | T/W Mar 21/22 |
| [ BCM] <br> | | [ BCM] <br> | ||
| [[20.109(S23):M2D3 |Sequence clones and transform into yeast cells]] <br> | | [[20.109(S23):M2D3 |Sequence clones and transform into yeast cells]] <br> | ||
| Line 93: | Line 99: | ||
|-- | |-- | ||
| M2D4 | | M2D4 | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
|-- | |-- | ||
| M2D5 | | M2D5 | ||
| R/F Mar 23/24 | | R/F Mar 23/24 | ||
| [ BCM] <br> | | [ BCM] <br> | ||
| − | | [[20.109(S23): | + | | [[20.109(S23):M2D4 |Determine transporter mutation and expression]] <br> |
| − | | [[20.109(S23):Homework# | + | | <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M2D4|Homework due]] |
|-- | |-- | ||
| | | | ||
| Line 116: | Line 119: | ||
| | | | ||
|-- | |-- | ||
| − | | | + | | M2D5 |
| R/F Apr 6/7 | | R/F Apr 6/7 | ||
| − | | | + | | [ BCM] <br> |
| − | | [[20.109(S23): | + | | [[20.109(S23):M2D5 |IF analysis and prepare conditions for metal incubation experiment]] <br> |
| − | | [ | + | | [[20.109(S23):Homework#Due_M2D5|Homework due]] |
|-- | |-- | ||
| M2D6 | | M2D6 | ||
| T/W Apr 11/12 | | T/W Apr 11/12 | ||
| [ BCM] <br> | | [ BCM] <br> | ||
| − | | [[20.109(S23): | + | | [[20.109(S23):Journal article presentation| <font color = #b3251c>'''Journal article presentations'''</font color>]] |
| − | | [ | + | | [ Blog post due] Sat, Apr 8 at 10 pm |
|-- | |-- | ||
| − | | | + | | M2D6 |
| R/F Apr 13/14 | | R/F Apr 13/14 | ||
| [ BCM] <br> | | [ BCM] <br> | ||
| − | | [[20.109(S23): | + | | [[20.109(S23):M2D6 |Analyze ICP-OES data and prepare for cytotoxicity experiment]] <br> |
| − | | [[20.109(S23):Homework# | + | | [[20.109(S23):Homework#Due_M2D5|Homework due]] |
|-- | |-- | ||
| | | | ||
| Line 140: | Line 143: | ||
| | | | ||
|-- | |-- | ||
| − | | | + | | M2D7 |
| R/F Apr 20/21 | | R/F Apr 20/21 | ||
| [ BCM] <br> | | [ BCM] <br> | ||
| − | | [[20.109(S23): | + | | [[20.109(S23):M2D7 |Determine cell survival of yeast exposed to metal]] <br> |
| − | | | + | | [[20.109(S23):Homework#Due_M2D7|Homework due]] |
|-- | |-- | ||
| − | | | + | | M2D8 |
| T/W Apr 25/26 | | T/W Apr 25/26 | ||
| − | | | + | | [BCM] <br> |
| − | | | + | | [[20.109(S23):M2D8 |Complete data analysis and organize Research article figures]] <br> |
| − | | | + | | <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M2D8|Homework due] |
|-- | |-- | ||
| M3D1 | | M3D1 | ||
Revision as of 15:35, 26 January 2023
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not enough time to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: Tuesday (T) and Thursday (R) 11 - 12 pm in 16-220
Laboratory section times: Tuesday (T) and Thursday (R) 1 - 5 pm or Wednesday (W) and Friday (F) 1 - 5 pm in 56-322
ABSENCE POLICY:
Absences from lecture: Attendance will be recorded for participation points throughout the semester. If absent, student is responsible for all information provided in lecture.
Absences from laboratory: Excused absences should be discussed with the Instructors as soon as possible. Because make-up laboratory time is not provided, attendance in another section may be required to complete the necessary experiments. Unexcused absences will result in a 1/3 of a letter grade deduction from the final grade on the major assignment for the module (for example, a B+ would become a B).
