Difference between revisions of "20.109(S20):Class data"

From Course Wiki
Jump to: navigation, search
(M1D4: Perform small molecule microarray (SMM) with TDP43 protein)
(Module 1: Screening ligand binders)
Line 21: Line 21:
 
*Grey Team: [[Media:Sp20 TR Grey SDSPAGE.HEIC |image here]]
 
*Grey Team: [[Media:Sp20 TR Grey SDSPAGE.HEIC |image here]]
  
 +
LiCOR Alexa 647 results:
 +
*Red Team: [[Media:Sp20 TR Red.tif |image here]]
 +
*Orange Team: [[Media:TR Orange Licor.tif |image here]]
 +
*Yellow Team: [[Media:TR Yellow Licor.tif |image here]]
 +
*Green Team: [[Media:TR Green Licor.tif |image here]]
 +
*Blue Team: [[Media:TR Red Licor.tif |image here]]
 +
*Pink Team: [[Media:TR Pink Licor.tif |image here]]
 +
*Purple Team: [[Media:TR Purple Licor.tif |image here]]
 +
*White Team: [[Media:TR White Licor.tif |image here]]
 +
*Grey Team: [[Media:TR Grey Licor.tif |image here]]
  
 
====WF Section====
 
====WF Section====
Line 36: Line 46:
 
*White Team: [[Media:WF White SDS.tif |image here]]
 
*White Team: [[Media:WF White SDS.tif |image here]]
 
*Silver Team: [[Media:WF Silver SDS.tif |image here]]
 
*Silver Team: [[Media:WF Silver SDS.tif |image here]]
 +
 +
LiCOR Alexa 647 results:
 +
*Red Team: [[Media:Sp20 WF Red.tif |image here]]
 +
*Orange Team: [[Media:Licor WF Orange.tif |image here]]
 +
*Yellow Team: [[Media:Licor WF Yellow.tif |image here]]
 +
*Green Team: [[Media:Licor WF Green.tif |image here]]
 +
*Blue Team: [[Media:Licor WF Blue.tif |image here]]
 +
*Pink Team: [[Media:Licor WF Pink.tif |image here]]
 +
*Purple Team: [[Media:Licor WF Purple.tif |image here]]
 +
*White Team: [[Media:Licor WF White.tif |image here]]
 +
*Silver Team: [[Media:Licor WF Silver.tif |image here]]
  
 
===M1D4: Perform small molecule microarray (SMM) with TDP43 protein===
 
===M1D4: Perform small molecule microarray (SMM) with TDP43 protein===

Revision as of 00:51, 27 February 2020

20.109(S20): Laboratory Fundamentals of Biological Engineering

Sp20 banner image v2.png

Spring 2020 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Screening ligand binding        2. Measuring gene expression        3. Engineering antibodies              


Module 1: Screening ligand binders

M1D3: Evaluate protein purity and concentration

TR Section

BCA Assay results (A562 measurements): linked here

SDS-PAGE results:

LiCOR Alexa 647 results:

WF Section

BCA Assay results (A562 measurements): linked here

SDS-PAGE results:

LiCOR Alexa 647 results:

M1D4: Perform small molecule microarray (SMM) with TDP43 protein

SMM slide assignments:

TR Team Slide barcodes WF Team Slide barcodes
Red 0011-03, 0011-04 Red 0011-11, 0013-11
Orange 0012-09, 0012-10 Orange 0013-2, 0014-9
Yellow 0012-05, 0012-06 Yellow 0013-1, 0014-1
Green 0012-03, 0012-04 Green 0011-7, 0013-10
Blue 0012-01, 0012-02 Blue 0013-12, 0014-12
Pink 0012-03, 0012-04 Pink 0014-11, 0014-8
Purple 0012-11, 0012-12 Purple 0013-9, 0014-10
White 0011-05, 0011-06 White 0013-7, 0014-7
Grey 0012-07, 0012-08 Silver 0011-8, 0011-12

Module 2: Measuring gene expression

==Module 3: Engineering antibodies==