20.109(F21):Fall 2021 schedule

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20.109(F21): Laboratory Fundamentals of Biological Engineering
Drawing provided by Marissa A., 20.109 student in Sp21 term.  Schematic generated using BioRender.

Fall 2021 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       Module 1: Genomic instability                          Module 2: Drug discovery       


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not enough time to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times:

  • Tuesday (T) and Thursday (R) 11 - 12 pm

Laboratory section times:

  • Tuesday (T) and Thursday (R) 1 - 5 pm
  • Wednesday (W) and Friday (F) 1 - 5 pm
MODULE DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
NLL
[[ |Slides]]
Orientation and laboratory tour
M1D1 BE
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Orientation quiz
Homework due
M1D2 BE
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Homework due
M1D3 BE
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Homework due
M1D4 BE
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Laboratory quiz
Homework due
M1D5 BE
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Homework due
T/W Mar 9/10 Student holiday
M1D6 BE
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Homework due
M1D7 BE
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Laboratory quiz
Homework due
M2D1 R/F Mar 18/19 AK
Slides
Perform protein purification protocol
T/R prelab
W/F prelab
Homework due
T/W Mar 23/24 Student holiday Data Summary draft due Wed, Mar 24 at 10 pm
M2D2 R/F Mar 25/26 AK
Slides
Assess purity and concentration of purified protein
T/R Prelab
W/F prelab
Homework due
Mini-presentation due Sun, Mar 28 at 10 pm
Blog post due Mon, Mar 29 at 10 pm
M2D3 T/W Mar 30/31 AK
Slides
Prepare and scan small molecule microarray (SMM) slides
T/R Prelab
W/F prelab
Homework due
M2D4 R/F Apr 1/2 AK
Slides
Analyze SMM data to identify putative small molecule binders
T/R Prelab
W/F prelab
Laboratory quiz
Homework due
Data Summary revision due Sun, Apr 4 at 10 pm
T/W Apr 6/7 Journal club presentations
R/F Apr 8/9 Journal club presentations Blog post due Sat, Apr 10 at 10 pm
M2D5 T/W Apr 13/14 AK
Slides
AI articles linked: here and here
Learn best practices for mammalian cell culture
T/R Prelab
W/F Prelab
Homework due
M2D6 R/F Apr 15/16 AK
Slides
Utilize cellular thermal shift assay (CETSA) to test putative small molecule binders
T/R Prelab
W/F Prelab
Homework due
T/W Apr 20/21 Student holiday
M2D7 R/F Apr 22/23 AK
Slides
Complete CETSA experiment and analyze data
T/R Prelab
W/F Prelab
Laboratory quiz
Homework due
M3D1 T/W Apr 27/28 AJ
Slides
Review IPC literature and examine structural characteristics
T/R Prelab
W/F Prelab
Homework due
M3D2 R/F Apr 29/30 AJ
Slides
Identify IPC mutations
T/R prelab
W/F prelab
Homework due
Research article due Sun, May 2 at 10 pm
Blog post due Mon, May 3 at 10 pm
M3D3 T/W May 4/5 AJ
Slides
Evaluate effect of mutations on IPC variants
T/R prelab
W/F prelab
Homework due
R/F May 6/7 Research proposal pitches
Develop Research proposal ideas and presentations
Prompts for discussion/homework
M3D4 T/W May 11/12 AJ
Slides
Design new IPC variant
T/R prelab
W/F prelab
Laboratory quiz
Homework due
R/F May 13/14 AJ
Slides
Mini-report due by 10 pm
T/W May 18/19 Research proposal presentations Blog post due Thu, May 20 at 10 pm
R May 20 Feedback session

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