20.109(F20):Module 3

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20.109(F20): Laboratory Fundamentals of Biological Engineering

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Fall 2020 schedule        FYI        Assignments        Homework        Communication |        Accessibility

       M1: Genomic instability        M2: Drug discovery        M3: Metabolic engineering       


Module 3

Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain, and Becky Meyer
TAs:

Overview

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Lab links: day by day

M3D1: Research project scope and experimental strategy
M3D2: Align gRNA sequences with genomic targets
M3D3: Analyze ethanol yield data
M3D4: Examine genomic features in gRNA-targeted sequences
M3D5: Design optimized gRNA sequence for enhanced ethanol production

Assignments

Research proposal presentation
Mini-report

References

CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nature Methods. (2013) 8: 2180-2196.

Metabolic engineering of Escherichia coli for production of mixed-acid fermentation end products. Frontiers in Bioengineering and Biotechnology. (2014) 2:1-12.

Notes for teaching faculty

Prep notes for M3
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