Module 3

Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain, and Becky Meyer
TAs:

Overview

At the core of scientific discovery is building on the research of others to push the field forward. To accomplish this an iterative process is used to answer research questions: manipulate, measure, mine and model, and modify.

Manipulate:

Measure:

Mine and Model:

Modify:

In Module 3 you will use this process to expand on the research completed by previous 109ers. In past iterations of this module students used a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli strain MG1655. Specifically, they used a CRISPR-based interference system (CRISPRi) to target genes within the anaerobic fermentative pathway in an effort to increase ethanol yield.

Image generated using BioRender.

Lab links: day by day

M3D1: Research project scope and experimental strategy
M3D2: Align gRNA sequences with genomic targets
M3D3: Analyze ethanol yield data
M3D4: Examine features in gRNA-targeted genomic sequences
M3D5: Design optimized gRNA sequence for enhanced ethanol production

Assignments

Research proposal presentation
Mini-report

References

CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nature Methods. (2013) 8: 2180-2196.

Metabolic engineering of Escherichia coli for production of mixed-acid fermentation end products. Frontiers in Bioengineering and Biotechnology. (2014) 2:1-12.

Notes for teaching faculty