Difference between revisions of "20.109(F20):Module 3"
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==References== | ==References== | ||
| − | [[Media:CRISPRi NatMeth2013.pdf| CRISPR interference (CRISPRi) for sequence-specific control of gene expression.]] ''Nature Methods.'' (2013) 8: 2180-2196. | + | [[Media:CRISPRi NatMeth2013.pdf| CRISPR interference (CRISPRi) for sequence-specific control of gene expression.]] ''Nature Methods.'' (2013) 8: 2180-2196. <br> |
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| + | [[Media:MetEngEcoli review.pdf |Metabolic engineering of ''Escherichia coli'' for production of mixed-acid fermentation end products.]] by Forster and Gescher, ''Frontiers in Bioengineering and Biotechnology.'' (2014)2:1-12. | ||
==Notes for teaching faculty== | ==Notes for teaching faculty== | ||
[[20.109(F20): Prep notes for M3| Prep notes for M3]] | [[20.109(F20): Prep notes for M3| Prep notes for M3]] | ||
Revision as of 19:21, 20 July 2020
Contents
Module 3
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Becky Meyer
TAs:
Overview
WRITE NEW PROJECT OVERVIEW
MAKE NEW OVERVIEW SCHEMATIC
Lab links: day by day
M3D1: Research project scope and experimental strategy
M3D2: Align gRNA sequences with genomic targets
M3D3: Analyze ethanol yield data
M3D4: Examine genomic features in gRNA-targeted sequences
M3D5: Design optimized gRNA sequence for enhanced ethanol production
Assignments
Research proposal presentation
Mini-report
References
CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nature Methods. (2013) 8: 2180-2196.
Metabolic engineering of Escherichia coli for production of mixed-acid fermentation end products. by Forster and Gescher, Frontiers in Bioengineering and Biotechnology. (2014)2:1-12.
