20.109(F20):M1D5

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20.109(F20): Laboratory Fundamentals of Biological Engineering

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Fall 2020 schedule        FYI        Assignments        Homework        Communication |        Accessibility

       M1: Genomic instability        M2: Drug discovery        M3: Metabolic engineering       


Part 2: Learn about the CometChip

As discussed in the prelab lecture, you will use two methods to assess DNA damage: the γH2AX assay and the CometChip. In the next laboratory session you will construct a CometChip; however, it is best to familiarize yourself with the procedure and assay before then.

Read the Abstract and Introduction in the following publication:

CometChip: A high-throughput 96-well platform for measuring DNA damage in microarrayed human cells. Journal of Visualized Experiments. (2014) 92: 1-11.

Answer the following questions (be sure to transfer the information to your laboratory notebook!):

  1. Why is it important to study DNA damage?
    • You can consider the information provided in lecture / prelab to answer this question.
  2. How does the CometChip estimate the level of DNA damage within cells?
  3. List two issues / problems with the comet assay?
  4. List two improvements provided by the CometChip assay?
Next, watch the following video to help you prepare for the next laboratory session: linked here.
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