20.109(F19): Prep notes for M3
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M3D1
24 hr preceding laboratory:
- seed MEF (250K) in T25 flasks, 1 per team
- cell expansion schedule for D1 and D2 prep
in tissue culture room (per team):
- 1 fresh T75 flask
- 3.5 mL PBS
- 1.5 mL trypsin
- 15 mL media
- not aliquoted: trypan blue
in main lab (per team):
- none