Difference between revisions of "20.109(F19): Prep notes for M3"
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Noreen Lyell (Talk | contribs) (→M3D1) |
Noreen Lyell (Talk | contribs) (→M3D2) |
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==M3D2== | ==M3D2== | ||
+ | |||
+ | '''48 hr preceding laboratory:''' | ||
+ | *seed MEF (2-3M) in T75 flasks, 4 per team | ||
+ | |||
+ | '''in tissue culture room (per team):''' | ||
+ | *25 mL PBS | ||
+ | *45 mL media | ||
+ | *not aliquoted: ligands | ||
+ | |||
+ | '''in main lab (per team):''' | ||
+ | *80 mL PBS | ||
+ | *8.5 mL PBS | ||
+ | *5 mL trypsin | ||
+ | *15 mL media | ||
+ | *not aliquoted: protease inhibitor, dry ice | ||
==M3D3== | ==M3D3== |
Revision as of 17:24, 1 November 2019
M3D1
24 hr preceding laboratory:
- seed MEF (250K) in T25 flasks, 1 per team
- cell expansion schedule for D1 and D2 prep
in tissue culture room (per team):
- 1 fresh T75 flask
- 3.5 mL PBS
- 1.5 mL trypsin
- 15 mL media
- not aliquoted: trypan blue
in main lab (per team):
- none
M3D2
48 hr preceding laboratory:
- seed MEF (2-3M) in T75 flasks, 4 per team
in tissue culture room (per team):
- 25 mL PBS
- 45 mL media
- not aliquoted: ligands
in main lab (per team):
- 80 mL PBS
- 8.5 mL PBS
- 5 mL trypsin
- 15 mL media
- not aliquoted: protease inhibitor, dry ice