Difference between revisions of "20.109(F19): Prep notes for M3"
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==M3D1== | ==M3D1== | ||
| + | |||
| + | '''24 hr preceding laboratory:''' | ||
| + | *seed MEF (250K) in T25 flasks, 1 per team | ||
| + | *[[Media:Fa19 M3 cell seeding schedule.xlsx | cell expansion schedule]] for D1 and D2 prep | ||
| + | |||
| + | '''in tissue culture room (per team):''' | ||
| + | *1 fresh T75 flask | ||
| + | *3.5 mL PBS | ||
| + | *1.5 mL trypsin | ||
| + | *15 mL media | ||
| + | *not aliquoted: trypan blue | ||
| + | |||
| + | '''in main lab (per team):''' | ||
| + | *none | ||
==M3D2== | ==M3D2== | ||
Revision as of 15:19, 1 November 2019
M3D1
24 hr preceding laboratory:
- seed MEF (250K) in T25 flasks, 1 per team
- cell expansion schedule for D1 and D2 prep
in tissue culture room (per team):
- 1 fresh T75 flask
- 3.5 mL PBS
- 1.5 mL trypsin
- 15 mL media
- not aliquoted: trypan blue
in main lab (per team):
- none
