Difference between revisions of "20.109(F19):Module 2"
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'''Lecturer:''' [http://be.mit.edu/directory/noreen-lyell Noreen Lyell]<br> | '''Lecturer:''' [http://be.mit.edu/directory/noreen-lyell Noreen Lyell]<br> | ||
| − | '''Instructors:''' [http://be.mit.edu/directory/noreen-lyell Noreen Lyell], [http://be.mit.edu/directory/leslie-mcclain Leslie McClain] and [ | + | '''Instructors:''' [http://be.mit.edu/directory/noreen-lyell Noreen Lyell], [http://be.mit.edu/directory/leslie-mcclain Leslie McClain] and [https://be.mit.edu/directory/becky-meyer Becky Meyer]<br> |
| − | '''TAs:''' | + | '''TAs:''' Colin Kim and Shelbi Parker<br> |
'''Lab manager:''' Hsinhwa Lee <br> | '''Lab manager:''' Hsinhwa Lee <br> | ||
Revision as of 19:10, 14 August 2019
Contents
Module 2
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Becky Meyer
TAs: Colin Kim and Shelbi Parker
Lab manager: Hsinhwa Lee
Overview
In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of E. coli such that either ethanol or acetate production is increased.
Lab links: day by day
M2D1: Complete in silico cloning of pdCas9
M2D2: Design gRNA for CRISPRi
M2D3: Generate gRNA plasmid
M2D4: Journal club I
M2D5: Confirm gRNA sequence in pgRNA
M2D6: Journal club II
M2D7: Induce CRISPRi system
M2D8: Measure fermentation products
Assignments
Research article
Journal club presentation
References
CRISPR interference (CRISPRi) for sequence-specific control of gene expression., Nature Methods. (2013) 8: 2180-2196.
