Difference between revisions of "20.109(F19):Journal club presentation (Day4 and 6)"

Overview and logistics

You will complete this assignment individually. Please review the 20.109 statement on collaboration and integrity as you proceed.

In Module 1, you delivered a mini-presentation that was focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. Reading, understanding, and explaining research related to your project are all important skills that will be important as you flex your scientist muscles.

As you prepare your talk be sure to review the resources provided on the Communication tab. In addition, please use the following link to view the full video from Susan McConnell: Designing effective scientific presentations.

On both journal club presentation days, we will meet in 16-336 and begin at 1:05 pm sharp. Presenters should arrive early and will be able to check their slides on the large screen.

Method of submission

Please submit your completed Journal club slides on the date of your presentation by 1 pm to Stellar, with filename Name_LabSection_JC.pptx (for example, NoreenLyell_TR_JC.pptx).

The choice of presentation order will be given to students who submitted their slides earliest.

Length and format of presentations

You will have 10 minutes to discuss the journal article you select. It may be very difficult, or impossible, to discuss all of the figures within the article adequately in only 10 minutes. Therefore, this assignment is not only to present the work, but also to identify the data that is most important to the conclusions. It is also critical to consider how your presentation 'flows' from one experiment to the next. As when you write your own research, you want to deliver a coherent story during your journal presentation.

Format considerations

The timing provided here is for a 10-minute presentation. For longer presentations, the slide count and proposed times may be increased proportionally.

Helpful hints

• A 10-minute talk is NOT a 30-minute talk given while racing through slides and speaking very quickly.
• Consider ways to transition from one slide to the next to ensure the information is tied together.
• Practice your presentation in front of people rather than in a room by yourself and practice several times!
• Familiarize yourself with using a laser pointer and/or slide changer if you will use one during the actual presentation.
• If you do choose to use a pointer, use it to direct attention to specific elements on the screen, rather than constantly gesturing in the general vicinity of your slide; otherwise, the audience will not know what's important. When you later make your own slides and figures, the apparent need for a pointer may actually mean you need to make a clearer slide.

Article selection

You may choose to select a journal article from those provided by the teaching faculty or you can select an article that is related to your Module 2 research from any peer-reviewed journal.

• If you choose an article from below, please "reserve" it by putting your (initials/lab section/team color) next to the listing here.
  • For visibility, please use the following format to sign up if possible, substituting in your own initials and team color: <font color = purple><b>[EF/WF/Purple]</b></font color>, which will look like [EF/WF/Purple]. Thanks!
• If you would like to discuss a paper not on the list below, please email it (as .pdf) to the teaching faculty (Noreen, Leslie, and Becky) with a brief description of the work.
  • The list of papers below is provided as a guideline for the types of papers that might be relevant for your presentation. You are not limited to the primary research articles on this list. The list is provided simply to give you an idea of the kinds of subjects that could make suitable presentations for the class. Feel free to search PubMed yourself to find articles of interest to you.
• The same paper may be presented by a T/R and a W/F student, but may only be presented once per section.

Please review the articles before making your final selection to ensure it is a paper that you find interesting and that you are comfortable presenting!

1. Birkholz et al. The autoregulator Aca2 mediated anti-CRISPR repression. (2019) Nucleic Acids Research. PMID: 31428783
2. Degrief et al. Preloading budding yeast with all-in-one CRIPSR/Cas9 vectors for easy and high-efficient genome editing. (2018) Journal of Biological Methods. PMID: 31453248 [CG/TR/Pink]
3. Dong et al. Systematic immunotherapy target discovery using genome-scale in vivo CRISPR screens in CD8 T cells. (2019) Cell. PMID: 31442407 [WG/TR/Orange]
4. English et al. Programmable CRISPR-responsive smart materials. (2019) Biomaterials. PMID: 31439791 [KG/TR/Purple]
5. Grunewald et al. CRISPR DNA base editors with reduced RNA off-target and self-editing activities. (2019) Nature Biotechnology. PMID: 31477922
6. Hanewich-Hollatz et al. Conditional guide RNAs: a programmable conditional regulation of CRISPR/Cas function in bacterial and mammalian cells via dynamic RNA nanotechnology. (2019) ACS Central Science. PMID: 31403072
7. Hu et al. Label-free CRISPR/Cas9 assay for site-specific nucleic acid detection. (2019) Analytical Chemistry. PMID: 31340642
8. Kang et al. Regulation of gene expression by altered promoter methylation using a CRISPR/Cas9-mediated epigenetic editing system. (2019) Nature Research. PMID: 31427598 [HH/TR/Purple]
9. Liang et al. A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules. (2019) Nature Communications. PMID: 31413315 [JA/TR/Green]
10. Liu et al. Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria. (2019) Nature Communications. PMID: 31451697 [AN/WF/WF]
11. Matsuda et al. Optimized CRISPR/Cas9-mediated in vivo genome engineering applicable to monitoring dynamics of endogenous proteins in the mouse neural tissues. (2019) Nature Research. PMID: 31383899
12. Nandy et al. Heat-shock-inducible CRISPR/Cas9 system generates heritable mutations in rice. (2019) Plant Direct. PMID: 31404128 [YK/TR/Green]
13. Nihongaki et al. A split CRISPR-Cpf1 platform for inducible genome editing and gene activation. (2019) Nature Chemical Biology. PMID: 31406371 [TN/WF/WF]
14. Ratner et al. Catalytically active Cas9 mediates transcriptional interference to facilitate bacterial virulence. (2019) Molecular Cell. PMID: 31439791
15. Soto-Perez et al. CRISPR-Cas system of a prevalent human gut bacterium reveals hyper-targeting against phages in a human virome catalog. (2019) Cell Host & Microbe. PMID: 31256988
16. Stanley et al. Anti-CRISPR-associated proteins are crucial repressors of anti-CRISPR transcription. (2019) Cell. PMID: 31474367 [PL/TR/Yellow]
17. Tuladhar et al. CRISPR-Cas9-based mutagenesis freqently provokes on-target mRNA misregulation. (2019) Nature Communications. PMID: 31492834 [FG/TR/Blue]
18. Wang et al. Cas12aVDet: a CRISPR/Cas12a-based platform for rapid and visual nucleic acid detection. (2019) Analytical Chemistry. PMID: 31460749 [SD/TR/Yellow] [EM/WF/WF]
19. Wang et al. CRISPR-mediated live imaging of genome editing and transcription. (2019) Science. PMID: 31488703 [DS/TR/Orange]
20. Young et al. A CRISPR platform for targeted in vivo screens identifies Toxoplasms gondii virulence factors in mice. (2019) Nature Communications. PMID: 31481656

Presentation day reservation

Please put your name under the day you wish to present. There are up to 6 slots on each day. Slot location does not determine speaker order.