20.109(F16): TA notes for M2

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20.109(F16): Laboratory Fundamentals of Biological Engineering

Engelward PNAS 2006.png

Schedule Fall 2016        Announcements        Assignments        Homework        Communication
       1. Measuring Genomic Instability        2. Manipulating Metabolism        3. Engineering Biomaterials              

M2D1

Per group, aliquot

  • 30 μL nuclease-free water
  • 5 μL pdCas9 (ideally at 500 ng/μL)

On front bench, have

  • restriction enzymes in -20 °C block
  • NEB buffers
  • list of 20.109-owned restriction enzymes

Fa16: all done by 5pm

M2D2

Per group,

  • pour 1/2 agarose gel (only 5 lanes needed)
  • thaw 4 samples of confirmation digests
  • aliquot 10 μL of 6X loading buffer
  • thaw 20 μL of 1 kb ladder

Call IDT to request expedited FedEx shipping of gRNA oligos.

Fa16: all done by 4:30pm

Should schedule BE Comm Lab workshop on Journal Club on this day.
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