20.109(F20):Module 3
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Contents
Module 3
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain, and Becky Meyer
TAs:
Overview
WRITE NEW PROJECT OVERVIEW
Lab links: day by day
M3D1: Research project scope and experimental strategy
M3D2: Align gRNA sequences with genomic targets
M3D3: Analyze ethanol yield data
M3D4: Examine genomic features in gRNA-targeted sequences
M3D5: Design optimized gRNA sequence for enhanced ethanol production
Assignments
Research proposal presentation
Mini-report
References
CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nature Methods. (2013) 8: 2180-2196.
Metabolic engineering of Escherichia coli for production of mixed-acid fermentation end products. Frontiers in Bioengineering and Biotechnology. (2014) 2:1-12.