20.109:Module 1

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20.109: Laboratory Fundamentals of Biological Engineering

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Module 1

Instructors: Drew Endy and Natalie Kuldell

TA: Sean Clarke

In this experimental module you will...

M13-coated coli from M. Russel
Map of M13 genome from M. Blaber

Module 1 Day 1: Start-up genome engineering
Module 1 Day 2: Agarose gel electrophoresis
Module 1 Day 3: DNA ligation and bacterial transformation
Module 1 Day 4: Examine candidate clones
Module 1 Day 5: Western analysis

Module 1 Day 6: Probe western
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