User contributions
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- 15:18, 8 February 2019 (diff | hist) . . (+8) . . 20.109(S19):Evaluate protein purity and concentration (Day4) (→Part 4b: Prepare Working Reagent (WR) and measuring protein concentration)
- 01:09, 7 February 2019 (diff | hist) . . (0) . . 20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1) (→Part 2: Construct pET21_FKBP12 in silico)
- 15:50, 6 February 2019 (diff | hist) . . (-41) . . 20.109(S19): Prep notes, orientation (→Station 2: making solutions) (current)
- 15:43, 6 February 2019 (diff | hist) . . (+6) . . 20.109(S19):Laboratory tour (→Part 2: Measuring liquids and mixing) (current)
- 15:41, 6 February 2019 (diff | hist) . . (+74) . . 20.109(S19):Laboratory tour (→Student registration and questionnaire)
- 15:39, 6 February 2019 (diff | hist) . . (-10) . . 20.109(S19):Communication (→Workshop Slides)
- 15:38, 6 February 2019 (diff | hist) . . (+245) . . 20.109(S19):Communication (→Workshop Slides)
- 15:35, 6 February 2019 (diff | hist) . . (-252) . . 20.109(S19):Communication (→Workshop Slides)
- 15:34, 6 February 2019 (diff | hist) . . (+1,613) . . 20.109(S19):Communication (→BE Communication Lab resources)
- 21:10, 5 February 2019 (diff | hist) . . (+49) . . 20.109(S19):Laboratory tour (→Station 6: Graphing your data (self-guided))
- 21:09, 5 February 2019 (diff | hist) . . (+10) . . 20.109(S19):Laboratory tour (→Student registration and questionnaire)
- 20:41, 5 February 2019 (diff | hist) . . (+45) . . 20.109(S19): Spring 2019 schedule
- 20:40, 5 February 2019 (diff | hist) . . (0) . . N File:Sp19 MOD0 LMMmarked.pdf (current)
- 18:28, 5 February 2019 (diff | hist) . . (-12) . . 20.109(S19):Laboratory tour (→Student registration and questionnaire)
- 20:39, 4 February 2019 (diff | hist) . . (-83) . . 20.109(S19):Purify protein for secondary assays(Day2) (→Part 4: Purify FKBP12 protein)
- 20:39, 4 February 2019 (diff | hist) . . (+7) . . 20.109(S19):Purify protein for secondary assays(Day2) (→Part 4: Purify FKBP12 protein)
- 20:38, 4 February 2019 (diff | hist) . . (+79) . . 20.109(S19):Purify protein for secondary assays(Day2) (→Part 4: Purify FKBP12 protein)
- 20:36, 4 February 2019 (diff | hist) . . (-7) . . 20.109(S19):Purify protein for secondary assays(Day2) (→Part 4: Purify FKBP12 protein)
- 20:35, 4 February 2019 (diff | hist) . . (+4) . . 20.109(S19):Purify protein for secondary assays(Day2) (→Part 4: Purify FKBP12 protein)
- 20:33, 4 February 2019 (diff | hist) . . (+81) . . 20.109(S19):Evaluate protein purity and concentration (Day4) (→Part 3: Visualize purified protein with polyacrylamide gel electrophoresis (PAGE))
- 20:31, 4 February 2019 (diff | hist) . . (+17) . . 20.109(S19):Evaluate protein purity and concentration (Day4) (→Part 4b: Prepare Working Reagent (WR) and measuring protein concentration)
- 20:31, 4 February 2019 (diff | hist) . . (+1) . . 20.109(S19):Evaluate protein purity and concentration (Day4) (→Part 4b: Prepare Working Reagent (WR) and measuring protein concentration)
- 20:29, 4 February 2019 (diff | hist) . . (-93) . . 20.109(S19):Evaluate protein purity and concentration (Day4) (→Part 4b: Prepare Working Reagent (WR) and measuring protein concentration)
- 20:22, 4 February 2019 (diff | hist) . . (+49) . . 20.109(S19):Evaluate protein purity and concentration (Day4) (→Part 3: Visualize purified protein with polyacrylamide gel electrophoresis (PAGE))
- 20:18, 4 February 2019 (diff | hist) . . (0) . . 20.109(S19):Evaluate protein purity and concentration (Day4) (→Part 2: Concentrate FKBP12 protein solution)
- 20:15, 4 February 2019 (diff | hist) . . (0) . . 20.109(S19):Evaluate protein purity and concentration (Day4) (→Protocols)
- 20:14, 4 February 2019 (diff | hist) . . (0) . . 20.109(S19):Evaluate protein purity and concentration (Day4) (→Protocols)
- 20:12, 4 February 2019 (diff | hist) . . (-33) . . 20.109(S19):Purify protein for secondary assays(Day2) (→Part 4: Purify FKBP12 protein)
- 20:08, 4 February 2019 (diff | hist) . . (+4) . . 20.109(S19):Purify protein for secondary assays(Day2) (→Part 4: Purify FKBP12 protein)
- 20:08, 4 February 2019 (diff | hist) . . (+8) . . 20.109(S19):Purify protein for secondary assays(Day2) (→Part 4: Purify FKBP12 protein)
- 20:07, 4 February 2019 (diff | hist) . . (-65) . . 20.109(S19):Purify protein for secondary assays(Day2) (→Reagents)
- 20:07, 4 February 2019 (diff | hist) . . (-390) . . 20.109(S19):Purify protein for secondary assays(Day2) (→Part 4: Purify FKBP12 protein)
- 19:58, 4 February 2019 (diff | hist) . . (+125) . . 20.109(S19):Purify protein for secondary assays(Day2) (→Part 3: Prepare Ni-NTA affinity column)
- 19:40, 4 February 2019 (diff | hist) . . (-174) . . 20.109(S19):Purify protein for secondary assays(Day2) (→Part 3: Prepare Ni-NTA affinity column)
- 19:39, 4 February 2019 (diff | hist) . . (+4) . . 20.109(S19):Purify protein for secondary assays(Day2) (→Part 2: Lyse BL21-A1 pET21_FKBP12 cells)
- 19:38, 4 February 2019 (diff | hist) . . (-20) . . 20.109(S19):Purify protein for secondary assays(Day2) (→Part 2: Lyse BL21-A1 pET21_FKBP12 cells)
- 19:32, 4 February 2019 (diff | hist) . . (+25) . . 20.109(S19):Purify protein for secondary assays(Day2) (→Part 1: Agarose gel electrophoresis of confirmation digests)
- 18:30, 4 February 2019 (diff | hist) . . (+115) . . 20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1) (→Part 3: Confirmation digest)
- 18:24, 4 February 2019 (diff | hist) . . (+28) . . 20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1) (→Reagents)
- 18:23, 4 February 2019 (diff | hist) . . (+71) . . 20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1) (→Reagents)
- 18:21, 4 February 2019 (diff | hist) . . (+27) . . 20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1) (→Part 4: Induce FKBP12 protein expression)
- 18:17, 4 February 2019 (diff | hist) . . (0) . . 20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1) (→Part 3: Confirmation digest)
- 18:16, 4 February 2019 (diff | hist) . . (-76) . . 20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1) (→Part 3: Confirmation digest)
- 18:13, 4 February 2019 (diff | hist) . . (-2) . . 20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1) (→Part 3: Confirmation digest)
- 18:12, 4 February 2019 (diff | hist) . . (+2) . . 20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1) (→Reagents)
- 18:11, 4 February 2019 (diff | hist) . . (-1) . . 20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1) (→Part 3: Confirmation digest)
- 18:11, 4 February 2019 (diff | hist) . . (0) . . File:20109enzymes.docx (Leslie McClain uploaded a new version of "File:20109enzymes.docx") (current)
- 18:10, 4 February 2019 (diff | hist) . . (0) . . N File:20109enzymes.docx
- 18:06, 4 February 2019 (diff | hist) . . (+22) . . 20.109(S19): Prep notes, orientation (→Station 1: pipetting)
- 18:05, 4 February 2019 (diff | hist) . . (-73) . . 20.109(S19): Prep notes, orientation (→Station 3: spectrophotometer)
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