Final Project -- Nathan S Lachenmyer
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Revision as of 16:02, 21 March 2012 by Nathan S Lachenmyer (Talk | contribs)
Contents
Confocal Microscopy
Literature
- Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth
- Confocal microscopy for biologists / Alan R. Hibbs.
- Handbook of biological confocal microscopy / editor, James B. Pawley.
Goals and Checkpoints
Goals
Key Factors | Proposed Technical Targets | Requirements for Success | Current Practice | Associated Technical Barriers |
---|---|---|---|---|
OCLSM Cost | < $8,000 | < $15,000 | > $100,000 commercial; ~$30,000 DIY | Cheap replacement for PMT & Galvo, etc. |
OCLSM Scan Time | 1 frame/second | 1 frame / 10 seconds | 10-50 fps | Fast scanning mirrors + ADC time |
Timeline
- 2 Weeks: Brightfield Confocal Microscopy
- 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images
- 3 Weeks: Laser Scanning Features
Proposed Design
Challenges
- Control software
- Getting good resolution