Difference between revisions of "Spring 2012:Leanna Morinishi Lab 2"
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=== Materials === | === Materials === | ||
* Pololu A4983 Stepper Motor Driver Carrier with Voltage Regulators<ref>[http://www.pololu.com/catalog/product/1202 A4983 Stepper Motor Driver Carrier with Voltage Regulators]</ref> | * Pololu A4983 Stepper Motor Driver Carrier with Voltage Regulators<ref>[http://www.pololu.com/catalog/product/1202 A4983 Stepper Motor Driver Carrier with Voltage Regulators]</ref> | ||
| − | * | + | * Thorlabs 8 mm Travel Stepper Motor Actuator<ref>[http://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=1881 8 mm Travel Stepper Motor Actuator]</ref> |
* Matlab | * Matlab | ||
* Matlab Image Acquisition Toolbox<ref>[http://www.mathworks.com/products/imaq/ Mathworks Image Acquisition Toolbox]</ref> | * Matlab Image Acquisition Toolbox<ref>[http://www.mathworks.com/products/imaq/ Mathworks Image Acquisition Toolbox]</ref> | ||
* Matlab Allied Vision Technologies Adaptor<ref>[http://www.alliedvisiontec.com/emea/products/software/3rd-party-solutions/mathworks.html Matlab Allied Vision Technologies Adaptor]</ref> | * Matlab Allied Vision Technologies Adaptor<ref>[http://www.alliedvisiontec.com/emea/products/software/3rd-party-solutions/mathworks.html Matlab Allied Vision Technologies Adaptor]</ref> | ||
| + | * National Instruments USB Data Acquisition<ref>[http://sine.ni.com/nips/cds/view/p/lang/en/nid/203223 National Instruments USB Data Acquisition 6210]</ref> | ||
| + | * Epi-fluorescence Microscope<ref>[[Lab_Manual:Optical_Microscopy]]</ref> with parts from Thorlabs | ||
| + | * Submicron beads, small enough to be considered point sources | ||
| + | |||
Deconvolution Methods for 3D Fluorescence Microscopy Images<ref>[http://www.ese.wustl.edu/~nehorai/paper/deconvolutions1.pdf Deconvolution Methods for 3D Fluorescence Microscopy Images - WUSTL 2006]</ref> | Deconvolution Methods for 3D Fluorescence Microscopy Images<ref>[http://www.ese.wustl.edu/~nehorai/paper/deconvolutions1.pdf Deconvolution Methods for 3D Fluorescence Microscopy Images - WUSTL 2006]</ref> | ||
Revision as of 06:56, 18 March 2012
Contents
Lab 2: Fluorescence Microscopy
Goals
In this module, I am interested in:
- Building and optimizing a fluorescent microscope
- Learning to control the microstepper
- Learning to talk to and control the camera
- Learning more about 3d deconvolution
- Test how it deconvolution is improved with actual PSF vs. theoretical PSF of the microscope
Methodology
Materials
- Pololu A4983 Stepper Motor Driver Carrier with Voltage Regulators[1]
- Thorlabs 8 mm Travel Stepper Motor Actuator[2]
- Matlab
- Matlab Image Acquisition Toolbox[3]
- Matlab Allied Vision Technologies Adaptor[4]
- National Instruments USB Data Acquisition[5]
- Epi-fluorescence Microscope[6] with parts from Thorlabs
- Submicron beads, small enough to be considered point sources
Deconvolution Methods for 3D Fluorescence Microscopy Images[7]
Results
Code
References
- ↑ A4983 Stepper Motor Driver Carrier with Voltage Regulators
- ↑ 8 mm Travel Stepper Motor Actuator
- ↑ Mathworks Image Acquisition Toolbox
- ↑ Matlab Allied Vision Technologies Adaptor
- ↑ National Instruments USB Data Acquisition 6210
- ↑ Lab_Manual:Optical_Microscopy
- ↑ Deconvolution Methods for 3D Fluorescence Microscopy Images - WUSTL 2006
