Difference between revisions of "Limits of Detection:Report Requirements"
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'''''You will gather data as a group; however, reports must be individually authored. Everything in your report, including computer code, must be entirely your own work.''''' | '''''You will gather data as a group; however, reports must be individually authored. Everything in your report, including computer code, must be entirely your own work.''''' | ||
− | <center>Answer the following questions:</center> | + | <center>'''Answer the following questions:'''</center> |
#Optical trap calibration | #Optical trap calibration | ||
##What is your estimate of the trap stiffness as a function of power as determined by the three different calibration methods (equipartition, PSD roll-off, and Stokes drag)? | ##What is your estimate of the trap stiffness as a function of power as determined by the three different calibration methods (equipartition, PSD roll-off, and Stokes drag)? |
Revision as of 03:29, 1 December 2011
- Before you go to the lab, read the optical trapping lab manual and the the Atomic Force Microscopy lab manual
- Attend an optical trap lab session during the week of 11/28-12/3.
- Attend an AFM lab session during the week of 12/5-12/9.
- The mini-lab report will be due at midnight on 12/13.
- The report should be answer-book style, like a problem set.
- Your report should be in PDF format, submitted to Stellar prior to the deadline.
- Submit all the computer code (.m files) that you used in your analysis as a single zip file to Stellar.
You will gather data as a group; however, reports must be individually authored. Everything in your report, including computer code, must be entirely your own work.
- Optical trap calibration
- What is your estimate of the trap stiffness as a function of power as determined by the three different calibration methods (equipartition, PSD roll-off, and Stokes drag)?
- Which method is superior? Explain why.
- What is the minimum detectable force as a function of power and bandwidth?
- AFM
- What is the stiffness of the cantilever you measured?
- How does the stiffness compare to the value computed from the cantilever's material properties?
- What is the smallest force you could measuer using that cantiliver?
- How you could modify the cantilever design to measure smaller forces? What is the smallest force that could practically be detected using a silicon nitride cantilever?
- How does the minimum detectable force compare to typical forces in biological systems (e.g. antibody/antigen binding, DNA hybridization, interdomain forces in proteins, etc.)?