Difference between revisions of "Talk:20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)"
From Course Wiki
MAXINE JONAS (Talk | contribs) (→IPC mutants (and primers) chosen by teams) |
MAXINE JONAS (Talk | contribs) |
||
| Line 5: | Line 5: | ||
*reverse primer: | *reverse primer: | ||
*restriction enzyme: | *restriction enzyme: | ||
| − | <font color = #FF0000>'''W/F RED'''</font color> | + | <br><font color = #FF0000>'''W/F RED'''</font color> |
*mutation: | *mutation: | ||
*forward primer: | *forward primer: | ||
*reverse primer: | *reverse primer: | ||
| − | *restriction enzyme: | + | *restriction enzyme: |
<br><font color = #FF6600>'''T/R ORANGE'''</font color> | <br><font color = #FF6600>'''T/R ORANGE'''</font color> | ||
*mutation: T78P | *mutation: T78P | ||
| Line 15: | Line 15: | ||
*reverse primer: | *reverse primer: | ||
*restriction enzyme: | *restriction enzyme: | ||
| − | <font color = #FFFF00>'''W/F YELLOW'''</font color> | + | <br><font color = #FFFF00>'''W/F YELLOW'''</font color> |
*mutation: D21A | *mutation: D21A | ||
*forward primer: | *forward primer: | ||
*reverse primer: | *reverse primer: | ||
| − | *restriction enzyme: | + | *restriction enzyme: |
<br><font color = #00FF00>'''T/R GREEN'''</font color> | <br><font color = #00FF00>'''T/R GREEN'''</font color> | ||
*mutation: D57H | *mutation: D57H | ||
Revision as of 19:21, 9 October 2015
IPC mutants (and primers) chosen by teams
T/R RED
- mutation: D132H
- forward primer:
- reverse primer:
- restriction enzyme:
W/F RED
- mutation:
- forward primer:
- reverse primer:
- restriction enzyme:
T/R ORANGE
- mutation: T78P
- forward primer:
- reverse primer:
- restriction enzyme:
W/F YELLOW
- mutation: D21A
- forward primer:
- reverse primer:
- restriction enzyme:
T/R GREEN
- mutation: D57H
- forward primer:
- reverse primer:
- restriction enzyme:
W/F GREEN
- mutation:
- forward primer:
- reverse primer:
- restriction enzyme:
T/R BLUE
- mutation: D132H
- forward primer:
- reverse primer:
- restriction enzyme:
W/F BLUE
- mutation:
- forward primer:
- reverse primer:
- restriction enzyme:
T/R PINK
- mutation: D132H
- forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3'
- reverse primer: 5'TCT GCT TCC CTT ATC ATT TC 3'
- restriction enzyme: NaeI (GCC / GGC)
W/F PINK
- mutation:
- forward primer:
- reverse primer:
- restriction enzyme:
