Difference between revisions of "Bryan Hernandez/20.109/Lab notebook/Module 1/Day 3"
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==Ligations and Transformations== | ==Ligations and Transformations== | ||
+ | --[http://openwetware.org/wiki/User:Bryanh |Bryanh] 17:17, 16 February 2007 (EST) | ||
==Purpose== | ==Purpose== | ||
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==Phage infection== | ==Phage infection== | ||
{| border="1" cellpadding="2" | {| border="1" cellpadding="2" | ||
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|'''Phage dilution'''||'''Phage Strain'''||'''PFUs Counted'''|| | |'''Phage dilution'''||'''Phage Strain'''||'''PFUs Counted'''|| | ||
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===Part 1: Ligation reactions=== | ===Part 1: Ligation reactions=== | ||
− | For your ligation, you will mix the M13K07 backbone you prepared with the annealed oligonucleotides. As control reactions you will also prepare a | + | For your ligation, you will mix the M13K07 backbone you prepared with the annealed oligonucleotides. As control reactions you will also prepare a |