Thermal Quenching Model

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Zipper et al says linear.

The Roche LightCycler paper [1] also shows a linear dependence, or at least it looks pretty linear (Fig. 2). Interesting -- they have a melting temp of about 85 degrees, with just normal DNA of 536bp length. We should try that. Anyway, my suspicion is that "linear" is a fine model of thermal quenching, and we should de-prioritize thermal quenching investigations.

Polymers & stuff

  • Polyvinyl alcohol
  • Polyvinylpyrrolidone
  • Polyethylene glycol (?)
  • Polysorbate (?)
  • Polyvinyl acetate (?)
  • Matrigel
  • Mineral oil
  • Glycerol
  • Gelatin
  • Agar(ose)